Extracellular FGF-1 inhibits cytoskeletal organization and promotes fibroblast motility.

Ding Q, Gladson CL, Guidry CR, Santoro SA, Dickeson SK, Shin JT, Thompson JA
Growth Factors. 2000 18 (2): 93-107

PMID: 11019781

Previous efforts from this laboratory have established that acidic fibroblast growth factor (FGF-1), either added exogenously or secreted as a biologically active protein, induces a transformed phenotype in primary murine fibroblasts. Experimental studies described here demonstrate that constitutive exposure to extracellular FGF-I results in reduced cell attachment to multiple ligands, inhibition of cytoskeletal organization, and reduced collagen contraction, despite no detectable change in integrin cell surface expression. In addition, FGF-1-transduced fibroblasts demonstrated a > 10-fold increase in migration, an observation correlated with increased tyrosine phosphorylation of p125FAK and p130CAS. Collectively, these results suggest that FGF-1-induced fibroblast transformation includes the involvement of specific FGF receptor-mediated signal transduction cascades targeted to cytoskeletal and focal adhesion structures.

MeSH Terms (10)

Animals Cell Adhesion Cell Movement Cell Transformation, Neoplastic Cytoskeleton Fibroblast Growth Factor 1 Fibroblast Growth Factors Fibroblasts Mice Mice, Inbred C57BL

Connections (2)

This publication is referenced by other Labnodes entities: