A novel member of the RING-finger gene family associated with reproductive tissues of the mosquito, Aaedes aegypti.

Zhao X, Smartt CT, Hillyer JF, Christensen BM
Insect Mol Biol. 2000 9 (3): 301-8

PMID: 10886414 · DOI:10.1046/j.1365-2583.2000.00189.x

The RING finger is a zinc-binding domain that is found in proteins from viruses, plants and animals. Here we report the characterization and tissue-specific expression of a mosquito gonadal protein gene, mgp, from the mosquito, Aedes aegypti. The putative gene product, MGP, contains two RING fingers, a B-box, and a hydrophobic core. These mosquito MGP structural motifs are highly conserved in proteins found in mouse and nematode. Northern blot analysis and in situ hybridization demonstrated the presence of multiple mgp RNA transcripts in male and female reproductive tissues. Expression of mgp in the ovary is constitutive, but an increase in message was observed in the ovaries of female mosquitoes previously exposed to a blood meal. These results suggest that MGP is a protein that might play a role(s) in mosquito gametogenesis.

MeSH Terms (17)

Aedes Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Southern DNA, Complementary Female Genes, Insect Insect Proteins Male Mice Molecular Sequence Data Ovary Sequence Analysis, DNA Testis Zinc Fingers

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