Isolation of an essential Schizosaccharomyces pombe gene, prp31(+), that links splicing and meiosis.

Bishop DT, McDonald WH, Gould KL, Forsburg SL
Nucleic Acids Res. 2000 28 (11): 2214-20

PMID: 10871341 · PMCID: PMC102626 · DOI:10.1093/nar/28.11.2214

We carried out a screen for mutants that arrest prior to premeiotic S phase. One of the strains we isolated contains a temperature-sensitive allele mutation in the fission yeast prp31(+) gene. The prp31-E1 mutant is defective in vegetative cell growth and in meiotic progression. It is synthetically lethal with prp6 and displays a pre-mRNA splicing defect at the restrictive temperature. We cloned the wild-type gene by complementation of the temperature-sensitive mutant phenotype. Prp31p is closely related to human and budding yeast PRP31 homologs and is likely to function as a general splicing factor in both vegetative growth and sexual differentiation.

MeSH Terms (19)

Amino Acid Sequence Cloning, Molecular Fungal Proteins Genes, Fungal Genes, Lethal Genetic Complementation Test Meiosis Molecular Sequence Data Mutation Phenotype Ribonucleoprotein, U4-U6 Small Nuclear Ribonucleoprotein, U5 Small Nuclear RNA Precursors RNA Splicing Saccharomyces Saccharomyces cerevisiae Proteins Schizosaccharomyces pombe Proteins Sequence Alignment S Phase

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