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Elongin from Saccharomyces cerevisiae.

Koth CM, Botuyan MV, Moreland RJ, Jansma DB, Conaway JW, Conaway RC, Chazin WJ, Friesen JD, Arrowsmith CH, Edwards AM
J Biol Chem. 2000 275 (15): 11174-80

PMID: 10753924 · DOI:10.1074/jbc.275.15.11174

Elongin is a transcription elongation factor that was first identified in mammalian systems and is composed of the three subunits, elongin A, B, and C. Sequence homologues of elongin A and elongin C, but not elongin B, were identified in the yeast genome. Neither yeast elongin A nor C sequence homologues was required for cell viability. The two gene products could be purified from yeast as a complex. A recombinant form of the complex, which could only be produced in bacteria if the gene products were co-expressed, was purified over several chromatographic steps. The complex did not stimulate transcription elongation by yeast RNA polymerase II. Using limited proteolysis, the N-terminal 144 residues of yeast elongin A were shown to be sufficient for interaction with yeast elongin C. The purified complex of yeast elongin C/elongin A(1-143) was analyzed using circular dichroism and nuclear magnetic spectroscopy. These studies revealed that yeast elongin A is unfolded but undergoes a dramatic modification of its structure in the presence of elongin C, and that elongin C forms a stable dimer in the absence of elongin A.

MeSH Terms (11)

Amino Acid Sequence Circular Dichroism Dimerization Elongin Fungal Proteins Magnetic Resonance Spectroscopy Molecular Sequence Data Protein Structure, Secondary Saccharomyces cerevisiae Transcription, Genetic Transcription Factors

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