Estrogen treatment in vivo increases keratinocyte growth factor expression in the mammary gland.

Pedchenko VK, Imagawa W
J Endocrinol. 2000 165 (1): 39-49

PMID: 10750034 · DOI:10.1677/joe.0.1650039

Proliferation and differentiation of mammary epithelia are regulated by the combined action of systemic hormones and locally derived paracrine growth factors. Keratinocyte growth factor (KGF) is a potential candidate stromal factor that may participate in the hormonal control of stromal/epithelial interactions. In this study, we have examined the in vivo effect of 17beta-estradiol (E) treatment on KGF expression in mammary glands of peripubertal (5-week-old) and mature (11-week-old) mice. Mice received subcutaneous injections of hormone after which KGF mRNA levels were assayed by ribonuclease protection analysis of mammary gland RNA. E treatment caused a dose- and time-dependent increase in KGF mRNA levels in intact mice from both age groups. Neither 17alpha-estradiol nor progesterone injection affected KGF mRNA levels. Comparison of the relative expression of KGF in parenchyma-free fat pads and in intact glands demonstrated that the basal and E-dependent KGF mRNA levels did not require the presence of mammary epithelium. ELISA assay of KGF tissue content demonstrated that concomitantly with an up-regulation of mRNA, E treatment also increased KGF protein in mammary glands from peripubertal and mature mice. These data show that E treatment stimulates both KGF mRNA and protein expression in mammary stroma in vivo and raises the possibility that KGF has a role in E-regulated mammary gland development.

MeSH Terms (17)

Age Factors Animals Dose-Response Relationship, Drug Enzyme-Linked Immunosorbent Assay Estradiol Female Fibroblast Growth Factor 7 Fibroblast Growth Factor 10 Fibroblast Growth Factors Growth Substances Mammary Glands, Animal Mice Mice, Inbred BALB C Ovariectomy RNA, Messenger Time Factors Up-Regulation

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