X-ray crystallographic analysis of the hydration of A- and B-form DNA at atomic resolution.

Egli M, Tereshko V, Teplova M, Minasov G, Joachimiak A, Sanishvili R, Weeks CM, Miller R, Maier MA, An H, Dan Cook P, Manoharan M
Biopolymers. 1998 48 (4): 234-52

PMID: 10699842 · DOI:10.1002/(SICI)1097-0282(1998)48:4<234::AID-BIP4>3.0.CO;2-H

We have determined single crystal structures of an A-DNA decamer and a B-DNA dodecamer at 0.83 and 0.95 A, respectively. The resolution of the former is the highest reported thus far for any right-handed nucleic acid duplex and the quality of the diffraction data allowed determination of the structure with direct methods. The structures reveal unprecedented details of DNA fine structure and hydration; in particular, we have reexamined the overall hydration of A- and B-form DNA, the distribution of water around phosphate groups, and features of the water structure that may underlie the B to A transition.

Copyright 2000 John Wiley & Sons, Inc.

MeSH Terms (7)

Crystallography, X-Ray DNA Nucleic Acid Conformation Oligodeoxyribonucleotides Phosphates Spermine Water

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