Reduced expression of the adherens junction protein cadherin-5 in a diabetic retina.

Davidson MK, Russ PK, Glick GG, Hoffman LH, Chang MS, Haselton FR
Am J Ophthalmol. 2000 129 (2): 267-9

PMID: 10682990 · DOI:10.1016/s0002-9394(99)00323-2

PURPOSE - Transvascular leakage occurs in diabetic retinopathy. The tight junction proteins occludin and zonula occludens-1 (ZO-1) and adherens junction protein cadherin-5 are critical to the maintenance of endothelial barrier. We report a comparison of junction protein expression in the normal and diabetic retina.

METHOD - Case report. Postmortem retinal cryosections were prepared from the left eye of a 73-year-old woman with diabetic retinopathy. Cryosections were immunostained for cadherin-5, occludin, and ZO-1 and compared with retinal cryosections from the right eye of a 72-year-old man with no progression of retinal disease.

RESULTS - Immunofluorescence showed positive retinal vessel staining for occludin and ZO-1 in both eyes and cadherin-5 in the normal eye but reduced cadherin-5 staining in the retinal vessels of the diabetic eye.

CONCLUSION - Increases in transvascular leakage observed in diabetic retinal vasculature may be associated with reduction in the expression of the critical adherens junction protein, cadherin-5.

MeSH Terms (17)

Aged Antigens, CD Blood-Retinal Barrier Cadherins Capillary Permeability Diabetic Retinopathy Endothelium, Vascular Female Fluorescent Antibody Technique, Indirect Humans Male Membrane Proteins Occludin Phosphoproteins Retina Retinal Vessels Zonula Occludens-1 Protein

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