Expression of the AML-1 oncogene shortens the G(1) phase of the cell cycle.

Strom DK, Nip J, Westendorf JJ, Linggi B, Lutterbach B, Downing JR, Lenny N, Hiebert SW
J Biol Chem. 2000 275 (5): 3438-45

PMID: 10652337 · DOI:10.1074/jbc.275.5.3438

The AML-1-encoded transcription factor, AML-1B, regulates numerous hematopoietic-specific genes. Inappropriate expression of AML-1-family proteins is oncogenic in cell culture systems and in mice. To understand the oncogenic functions of AML-1, we established cell lines expressing AML-1B to examine the role of AML-1 in the cell cycle. DNA content analysis and bromodeoxyuridine pulse-chase studies indicated that entry into the S phase of the cell cycle was accelerated by up to 4 h in AML-1B-expressing 32D.3 myeloid progenitor cells as compared with control cells or cells expressing E2F-1. However, AML-1B was not able to induce continued cell cycle progression in the absence of growth factors. The DNA binding and transactivation domains of AML-1B were required for altering the cell cycle. Thus, AML-1B is the first transcription factor that affects the timing of the mammalian cell cycle.

MeSH Terms (13)

Animals Cell Cycle Cell Line Core Binding Factor Alpha 2 Subunit DNA-Binding Proteins Flow Cytometry G1 Phase Gene Expression Regulation Humans Mice Proto-Oncogene Proteins Transcription Factors Transfection

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