Quasispecies development of Helicobacter pylori observed in paired isolates obtained years apart from the same host.

Kuipers EJ, Israel DA, Kusters JG, Gerrits MM, Weel J, van Der Ende A, van Der Hulst RW, Wirth HP, Höök-Nikanne J, Thompson SA, Blaser MJ
J Infect Dis. 2000 181 (1): 273-82

PMID: 10608776 · PMCID: PMC2766531 · DOI:10.1086/315173

Helicobacter pylori isolates show greater genetic diversity than other bacterial species studied, but the basis for this phenomenon is unknown. Whether detectable genomic mutation appears within an H. pylori population during persistent colonization was investigated. Paired H. pylori populations obtained across 7- to 10-year intervals from 13 patients were characterized by use of methods including polymerase chain reaction (PCR) genotyping for cagA, vacA, iceA, recA, and IS605; random arbitrarily primed DNA (RAPD)-PCR and amplified fragment length polymorphism (AFLP) analysis; and ELISA, to determine Lewis phenotypes. Genotyping, including recA sequence analysis, revealed that initial and follow-up populations represented the same population in 11 patients (85%). Nevertheless, distinct dissimilarities were shown within each of these 11 pairs by both RAPD-PCR and AFLP analyses. During follow-up, Lewis-y levels, but not Lewis-x levels, decreased significantly. The changes detected by RAPD-PCR and AFLP indicate that genetic drift occurs within H. pylori populations over the course of years of colonization of a single host.

MeSH Terms (18)

Adult Aged Aged, 80 and over Antigens, Bacterial Bacterial Outer Membrane Proteins Bacterial Proteins DNA Transposable Elements Evolution, Molecular Female Genetic Variation Genome, Bacterial Genotype Helicobacter Infections Helicobacter pylori Humans Male Middle Aged Random Amplified Polymorphic DNA Technique

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