Vascular endothelial growth factor levels and induction of permeability in malignant pleural effusions.

Zebrowski BK, Yano S, Liu W, Shaheen RM, Hicklin DJ, Putnam JB, Ellis LM
Clin Cancer Res. 1999 5 (11): 3364-8

PMID: 10589746

Vascular endothelial growth factor (VEGF) is an important mediator of angiogenesis and vascular permeability. We hypothesized that malignant pleural effusions may contain high levels of VEGF protein as well as other cytokines implicated in these processes. Pleural effusions cytologically proven to be malignant were collected from 39 patients with various types of cancer, and VEGF, interleukin-8, and angiogenin levels in the effusions were determined by immunoassay. Negative controls were nonmalignant ascites and serum samples from healthy individuals. VEGF levels were significantly higher than those of control samples in pleural effusions secondary to breast, mesothelioma, and non-small cell lung cancer and when all malignant pleural effusion samples were pooled. Neither interleukin-8 nor angiogenin levels were elevated in malignant pleural effusions relative to the control samples. Vascular permeability, which was measured by using the Miles assay in nude mice, was increased proportionately with VEGF levels in the malignant pleural effusions; this increase in permeability induced by injection of recombinant VEGF or the malignant effusions was reduced by pretreating the mice with a VEGF receptor antibody.

MeSH Terms (27)

Angiogenesis Inducing Agents Animals Breast Neoplasms Capillary Permeability Carcinoma, Non-Small-Cell Lung Endothelial Growth Factors Female Humans Interleukin-8 Lung Neoplasms Lymphokines Lymphoma Male Mesothelioma Mice Mice, Nude Pleural Effusion, Malignant Proteins Receptor Protein-Tyrosine Kinases Receptors, Growth Factor Receptors, Vascular Endothelial Growth Factor Recombinant Proteins Ribonuclease, Pancreatic Sarcoma Sarcoma Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors

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