Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing.

Burns CG, Ohi R, Krainer AR, Gould KL
Proc Natl Acad Sci U S A. 1999 96 (24): 13789-94

PMID: 10570151 · PMCID: PMC24143 · DOI:10.1073/pnas.96.24.13789

The conserved CDC5 family of Myb-related proteins performs an essential function in cell cycle control at G(2)/M. Although c-Myb and many Myb-related proteins act as transcription factors, herein, we implicate CDC5 proteins in pre-mRNA splicing. Mammalian CDC5 colocalizes with pre-mRNA splicing factors in the nuclei of mammalian cells, associates with core components of the splicing machinery in nuclear extracts, and interacts with the spliceosome throughout the splicing reaction in vitro. Furthermore, genetic depletion of the homolog of CDC5 in Saccharomyces cerevisiae, CEF1, blocks the first step of pre-mRNA processing in vivo. These data provide evidence that eukaryotic cells require CDC5 proteins for pre-mRNA splicing.

MeSH Terms (20)

3T3 Cells Animals Cell Cycle Proteins Cell Nucleus Fungal Proteins Humans Mice Protein-Serine-Threonine Kinases Protein Kinases Proto-Oncogene Proteins Proto-Oncogene Proteins c-myb RNA-Binding Proteins RNA Precursors RNA Splicing Saccharomyces cerevisiae Saccharomyces cerevisiae Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Spliceosomes Subcellular Fractions

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