On the contractile function of protein phosphatases in isolated human coronary arteries.

Knapp J, Bokník P, Deng MC, Huke S, Lüss I, Klein-Wiele O, Linck B, Lüss H, Müller FU, Nacke P, Scheld HH, Schmitz W, Vahlensieck U, Neumann J
Naunyn Schmiedebergs Arch Pharmacol. 1999 360 (4): 464-72

PMID: 10551284 · DOI:10.1007/s002109900124

It is unknown whether protein phosphatases types 1 and 2A are present in and can regulate the tone of human vascular tissue. The expression and possible function of serine/threonine protein phosphatases (PP) type 1 (PP1) and type 2A (PP2A) were studied in isolated human coronary arteries. Catalytic subunits of PPI and PP2A were identified by means of phosphatase activity measurement in tissue homogenates, by separation of enriched extracts through affinity column chromatography, by immunoblotting with specific antibodies, by hybridization of mRNA with specific DNA probes and PCR of reverse transcribed mRNA. Based on these methods, the catalytic subunits of PP1(alpha,beta,gamma) and PP2A(alpha,beta) were identified. Appropriately, cantharidin, an inhibitor of PP1 and PP2A, increased basal tone of human isolated coronary artery rings with an EC50 of about 16 micromol/l by increasing the phosphorylation state of the regulatory light chains of myosin. In summary, PP1 and PP2A are expressed in human coronary arteries and they can alter vascular tone.

MeSH Terms (12)

Chromatography, Affinity Coronary Vessels Humans Immunoblotting Muscle, Smooth, Vascular Muscle Contraction Myocardium Myosins Nucleic Acid Hybridization Phosphoprotein Phosphatases Phosphoric Monoester Hydrolases Phosphorylation

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