The zinc finger protein NRIF interacts with the neurotrophin receptor p75(NTR) and participates in programmed cell death.

Casademunt E, Carter BD, Benzel I, Frade JM, Dechant G, Barde YA
EMBO J. 1999 18 (21): 6050-61

PMID: 10545116 · PMCID: PMC1171670 · DOI:10.1093/emboj/18.21.6050

NRIF (neurotrophin receptor interacting factor) is a ubiquitously expressed zinc finger protein of the Kr├╝ppel family which interacts with the neurotrophin receptor p75(NTR). The interaction was first detected in yeast and then biochemically confirmed using recombinant GST-NRIF fusions and p75(NTR) expressed by eukaryotic cells. Transgenic mice carrying a deletion in the exon encoding the p75(NTR)-binding domain of NRIF display a phenotype which is strongly dependent upon genetic background. While at the F(2 )generation there is only limited (20%) embryonic lethality, in a congenic BL6 strain nrif(-/-) mice cannot survive beyond E12, but are viable and healthy to adulthood in the Sv129 background. The involvement of NRIF in p75(NTR)/NGF-mediated developmental cell death was examined in the mouse embryonic neural retina. Disruption of the nrif gene leads to a reduction in cell death which is quantitatively indistinguishable from that observed in p75(NTR)(-/-) and ngf(-/-) mice. These results indicate that NRIF is an intracellular p75(NTR)-binding protein transducing cell death signals during development.

MeSH Terms (24)

Amino Acid Sequence Animals Apoptosis Binding Sites Carrier Proteins Cell Line Cloning, Molecular DNA-Binding Proteins Embryonic and Fetal Development Gene Targeting Intracellular Signaling Peptides and Proteins Kruppel-Like Transcription Factors Mice Mice, Knockout Molecular Sequence Data Protein Binding Receptor, Nerve Growth Factor Recombinant Fusion Proteins Repressor Proteins Retina RNA, Messenger Transcription Factors Transfection Zinc Fingers

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