HP0333, a member of the dprA family, is involved in natural transformation in Helicobacter pylori.

Ando T, Israel DA, Kusugami K, Blaser MJ
J Bacteriol. 1999 181 (18): 5572-80

PMID: 10482496 · PMCID: PMC94075 · DOI:10.1128/JB.181.18.5572-5580.1999

Helicobacter pylori is naturally competent for DNA transformation, but the mechanism by which transformation occurs is not known. For Haemophilus influenzae, dprA is required for transformation by chromosomal but not plasmid DNA, and the complete genomic sequence of H. pylori 26695 revealed a dprA homolog (HP0333). Examination of genetic databases indicates that DprA homologs are present in a wide variety of bacterial species. To examine whether HP0333 has a function similar to dprA of H. influenzae, HP0333, present in each of 11 strains studied, was disrupted in two H. pylori isolates. For both mutants, the frequency of transformation by H. pylori chromosomal DNA was markedly reduced, but not eliminated, compared to their wild-type parental strains. Mutation of HP0333 also resulted in a marked decrease in transformation frequency by a shuttle plasmid (pHP1), which differs from the phenotype described in H. influenzae. Complementation of the mutant with HP0333 inserted in trans in the chromosomal ureAB locus completely restored the frequency of transformation to that of the wild-type strain. Thus, while dprA is required for high-frequency transformation, transformation also may occur independently of DprA. The presence of DprA homologs in bacteria known not to be naturally competent suggests a broad function in DNA processing.

MeSH Terms (20)

Amino Acid Sequence Bacterial Proteins Base Sequence Chromosomes, Bacterial Consensus Sequence DNA Primers Escherichia coli Evolution, Molecular Genetic Complementation Test Genotype Haemophilus influenzae Helicobacter pylori Membrane Proteins Molecular Sequence Data Mutagenesis, Insertional Phylogeny Restriction Mapping Sequence Alignment Sequence Homology, Amino Acid Transformation, Bacterial

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