Src induces morphological changes in A431 cells that resemble epidermal differentiation through an SH3- and Ras-independent pathway.

Jin F, Reynolds AB, Hines MD, Jensen PJ, Johnson KR, Wheelock MJ
J Cell Sci. 1999 112 ( Pt 17): 2913-24

PMID: 10444386

The role of Src family tyrosine kinases in cellular proliferation is well established; however, their role in cellular differentiation is less well understood. In this study we have investigated the role played by Src in the differentiation of squamous epithelial cells. Transfection of activated Src into A431 cells resulted in morphological changes that resembled epidermal differentiation. When we used Src mutants to characterize the observed phenotypic changes, we found that protein tyrosine kinase activity, correct membrane localization and the activity of the SH2 domain were required, but the SH3 domain was not. Furthermore, downstream activity of Ras was not required for the Src-mediated changes in A431 cells.

MeSH Terms (19)

Biomarkers Carcinoma, Squamous Cell Cell Adhesion Cell Differentiation Cell Size Epidermis Female Genes, src Humans Oncogene Protein pp60(v-src) Phosphorylation Protein-Tyrosine Kinases Protein Processing, Post-Translational Proto-Oncogene Proteins p21(ras) Recombinant Fusion Proteins src Homology Domains Transfection Tumor Cells, Cultured Uterine Cervical Neoplasms

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