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COX-2 compensation in the uterus of COX-1 deficient mice during the pre-implantation period.

Reese J, Brown N, Paria BC, Morrow J, Dey SK
Mol Cell Endocrinol. 1999 150 (1-2): 23-31

PMID: 10411296 · DOI:10.1016/s0303-7207(99)00033-7

Prostaglandins (PGs) produced by cyclooxygenase (COX) participate in many aspects of female reproduction. The two isoforms of cyclooxygenase, COX-1 and COX-2, have distinct expression patterns in the mouse uterus during the peri-implantation period and suggest their independent contribution to uterine PGs. Using wild type and COX-1(-/-) mice, we examined the role of COX-1-derived PGs on day 4 of pregnancy, when its expression is maximal. Uterine vascular permeability was measured by 125I-labeled bovine serum albumin (BSA) uptake, and PG content was measured by gas chromatography-mass spectrometry. Vascular permeability and PG concentrations were reduced in COX-1(-/-) mice, but by less than the expected amount. After ovariectomy, uterine vascular permeability declined in both groups, but returned to baseline in wild type and was exaggerated in COX-1(-/-) females after treatment with ovarian steroids. Most importantly, COX-1(-/-) uteri displayed COX-2 expression on the morning of day 4, when COX-2 is normally absent. This hybridization pattern resembles the native expression of COX-1, and may partially offset the loss of COX-1-derived PGs. These data indicate that COX-1-derived PGs are important during uterine preparation for implantation, and that COX-2 compensation occurs in the absence of COX-1.

MeSH Terms (15)

Animals Cattle Cyclooxygenase 1 Cyclooxygenase 2 Embryonic Development Female Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Isoenzymes Membrane Proteins Mice Mice, Knockout Pregnancy Prostaglandin-Endoperoxide Synthases Uterus

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