p120(ctn) acts as an inhibitory regulator of cadherin function in colon carcinoma cells.

Aono S, Nakagawa S, Reynolds AB, Takeichi M
J Cell Biol. 1999 145 (3): 551-62

PMID: 10225956 · PMCID: PMC2185070 · DOI:10.1083/jcb.145.3.551

p120(ctn) binds to the cytoplasmic domain of cadherins but its role is poorly understood. Colo 205 cells grow as dispersed cells despite their normal expression of E-cadherin and catenins. However, in these cells we can induce typical E-cadherin-dependent aggregation by treatment with staurosporine or trypsin. These treatments concomitantly induce an electrophoretic mobility shift of p120(ctn) to a faster position. To investigate whether p120(ctn) plays a role in this cadherin reactivation process, we transfected Colo 205 cells with a series of p120(ctn) deletion constructs. Notably, expression of NH2-terminally deleted p120(ctn) induced aggregation. Similar effects were observed when these constructs were introduced into HT-29 cells. When a mutant N-cadherin lacking the p120(ctn)-binding site was introduced into Colo 205 cells, this molecule also induced cell aggregation, indicating that cadherins can function normally if they do not bind to p120(ctn). These findings suggest that in Colo 205 cells, a signaling mechanism exists to modify a biochemical state of p120(ctn) and the modified p120(ctn) blocks the cadherin system. The NH2 terminus-deleted p120(ctn) appears to compete with the endogenous p120(ctn) to abolish the adhesion-blocking action.

MeSH Terms (29)

Alkaloids alpha Catenin Benzoquinones beta Catenin Binding Sites Cadherins Catenins Cell Adhesion Molecules Cell Aggregation Cytoskeletal Proteins DNA, Complementary DNA Primers Electrophoresis Enzyme Inhibitors Gene Expression Regulation, Neoplastic Genistein HT29 Cells Humans Kidney Lactams, Macrocyclic Membrane Glycoproteins Mucin-1 Naphthalenes Phosphoproteins Quinones Rifabutin Staurosporine Trans-Activators Transfection

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