Peptide dependency of alloreactive CD4+ T cell responses.

Mendiratta SK, Kovalik JP, Hong S, Singh N, Martin WD, Van Kaer L
Int Immunol. 1999 11 (3): 351-60

PMID: 10221647 · DOI:10.1093/intimm/11.3.351

Alloreactivity, the capacity of a large number of T lymphocytes to react with foreign MHC molecules, represents the cellular basis for the rejection of tissue grafts. Although it was originally assumed that the TCR of alloreactive T cells focus their recognition on the polymorphic residues that differ between the MHC molecules of responder and stimulator cells, studies in the MHC class I system have clearly demonstrated that MHC-bound peptides can influence this interaction. It remains unclear, however, whether peptides play an equally important role for the recognition of MHC class II molecules by alloreactive CD4+ T cells. Another issue that remains unresolved is the overall frequency of peptide-dependent versus peptide-independent alloreactive T cells. We have addressed these questions with antigen-presenting cells (APC) from H2-M mutant mice that predominantly express a single MHC class II-peptide complex, H2-Ab bound by a peptide (CLIP) derived from the class II-associated invariant chain. APC from these mice were used as targets and stimulators for alloreactive CD4+ T cells. Results demonstrated that the vast majority of CD4+ alloreactive T cells recognize MHC class II molecules in a peptide-dependent fashion.

MeSH Terms (15)

Amino Acid Sequence Animals Antigen-Presenting Cells Antigens, Differentiation, B-Lymphocyte CD4-Positive T-Lymphocytes Cell Line H-2 Antigens Histocompatibility Antigens Class II Hybridomas Lymphocyte Culture Test, Mixed Mice Mice, Mutant Strains Molecular Sequence Data Oligopeptides T-Lymphocyte Subsets

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