The schizosaccharomyces pombe dim1(+) gene interacts with the anaphase-promoting complex or cyclosome (APC/C) component lid1(+) and is required for APC/C function.

Berry LD, Feoktistova A, Wright MD, Gould KL
Mol Cell Biol. 1999 19 (4): 2535-46

PMID: 10082519 · PMCID: PMC84046 · DOI:10.1128/mcb.19.4.2535

The Schizosaccharomyces pombe dim1(+) gene is required for entry into mitosis and for chromosome segregation during mitosis. To further understand dim1p function, we undertook a synthetic lethal screen with the temperature-sensitive dim1-35 mutant and isolated lid (for lethal in dim1-35) mutants. Here, we describe the temperature-sensitive lid1-6 mutant. At the restrictive temperature of 36 degrees C, lid1-6 mutant cells arrest with a "cut" phenotype similar to that of cut4 and cut9 mutants. An epitope-tagged version of lid1p is a component of a multiprotein approximately 20S complex; the presence of lid1p in this complex depends upon functional cut9(+). lid1p-myc coimmunoprecipitates with several other proteins, including cut9p and nuc2p, and the presence of cut9p in a 20S complex depends upon the activity of lid1(+). Further, lid1(+) function is required for the multiubiquitination of cut2p, an anaphase-promoting complex or cyclosome (APC/C) target. Thus, lid1p is a component of the S. pombe APC/C. In dim1 mutants, the abundances of lid1p and the APC/C complex decline significantly, and the ubiquitination of an APC/C target is abolished. These data suggest that at least one role of dim1p is to maintain or establish the steady-state level of the APC/C.

MeSH Terms (20)

Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome Apc3 Subunit, Anaphase-Promoting Complex-Cyclosome Apc6 Subunit, Anaphase-Promoting Complex-Cyclosome Cell Cycle Proteins Cloning, Molecular Fungal Proteins Genes, Fungal Ligases Molecular Sequence Data Nuclear Proteins Protein Binding Protein Processing, Post-Translational Schizosaccharomyces Schizosaccharomyces pombe Proteins Sequence Analysis, DNA Sequence Homology, Amino Acid Ubiquitin-Protein Ligase Complexes Ubiquitin-Protein Ligases Ubiquitins

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