Evidence for F-actin-dependent and -independent mechanisms involved in assembly and stability of the medial actomyosin ring in fission yeast.

Naqvi NI, Eng K, Gould KL, Balasubramanian MK
EMBO J. 1999 18 (4): 854-62

PMID: 10022828 · PMCID: PMC1171178 · DOI:10.1093/emboj/18.4.854

Cell division in a number of eukaryotes, including the fission yeast Schizosaccharomyces pombe, is achieved through a medially placed actomyosin-based contractile ring. Although several components of the actomyosin ring have been identified, the mechanisms regulating ring assembly are still not understood. Here, we show by biochemical and mutational studies that the S.pombe actomyosin ring component Cdc4p is a light chain associated with Myo2p, a myosin II heavy chain. Localization of Myo2p to the medial ring depended on Cdc4p function, whereas localization of Cdc4p at the division site was independent of Myo2p. Interestingly, the actin-binding and motor domains of Myo2p are not required for its accumulation at the division site although the motor activity of Myo2p is essential for assembly of a normal actomyosin ring. The initial assembly of Myo2p and Cdc4p at the division site requires a functional F-actin cytoskeleton. Once established, however, F-actin is not required for the maintenance of Cdc4p and Myo2p medial rings, suggesting that the attachment of Cdc4p and Myo2p to the division site involves proteins other than actin itself.

MeSH Terms (22)

Actins Actomyosin Bacterial Proteins Carrier Proteins Cell Cycle Proteins Cell Division Cytoskeletal Proteins F-Box Proteins Fluorescent Antibody Technique Fungal Proteins Microscopy, Fluorescence Mutation Myosin Heavy Chains Myosin Light Chains Myosins Myosin Type II Myosin Type V Protein Binding Saccharomyces cerevisiae Proteins Schizosaccharomyces Schizosaccharomyces pombe Proteins Ubiquitin-Protein Ligases

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