, a bio/informatics shared resource is still "open for business" - Visit the CDS website


Other search tools

About this data

The publication data currently available has been vetted by Vanderbilt faculty, staff, administrators and trainees. The data itself is retrieved directly from NCBI's PubMed and is automatically updated on a weekly basis to ensure accuracy and completeness.

If you have any questions or comments, please contact us.

Results: 941 to 949 of 949

Publication Record

Connections

Activation of Ca(2+)-dependent currents in cultured rat dorsal root ganglion neurones by a sperm factor and cyclic ADP-ribose.
Currie KP, Swann K, Galione A, Scott RH
(1992) Mol Biol Cell 3: 1415-25
MeSH Terms: Adenosine Diphosphate Ribose, Animals, Animals, Newborn, Barium, Caffeine, Calcium, Calcium Channels, Cells, Cultured, Cricetinae, Cyclic ADP-Ribose, Ganglia, Spinal, Male, Membrane Potentials, Neurons, Proteins, Rats, Rats, Wistar, Ruthenium Red, Spermatozoa, Swine, Tissue Extracts
Show Abstract · Added March 30, 2013
The effects of intracellular application of two novel Ca2+ releasing agents have been studied in cultured rat dorsal root ganglion (DRG) neurones by monitoring Ca(2+)-dependent currents as a physiological index of raised free cytosolic Ca2+ ([Ca2+]i). A protein based sperm factor (SF) extracted from mammalian sperm, has been found to trigger Ca2+ oscillations and to sensitize unfertilized mammalian eggs to calcium induced calcium release (CICR). In this study intracellular application of SF activated Ca(2+)-dependent currents in approximately two-thirds of DRG neurones. The SF induced activity was abolished by heat treatment, attenuated by increasing the intracellular Ca2+ buffering capacity of the cells and persisted when extracellular Ca2+ was replaced by Ba2+. In addition, activity could be triggered or potentiated by loading the cells with Ca2+ by activating a series of voltage-gated Ca2+ currents. Ca(2+)-activated inward current activity was also generated by intracellular application of cyclic ADP-ribose (cADPR), a metabolite of NAD+, which causes Ca2+ release in sea urchin eggs. This activity could also be enhanced by loading the cells with Ca2+. The cADPR induced activity, but not the SF induced activity, was abolished by depleting the caffeine sensitive Ca2+ store. Ruthenium red markedly attenuated SF induced activity but had little action on cADPR induced activity or caffeine induced activity. Our results indicate that both SF and cADPR release intracellular Ca2+ pools in DRG neurones and that they appear to act on subtly distinct stores or distinct intracellular Ca2+ release mechanisms, possibly by modulating CICR.
0 Communities
1 Members
0 Resources
21 MeSH Terms
Factor XIII: a study using polarization of fluorescence of the activation of the enzyme by calcium and by substrate [proceedings].
Holbrook JJ, Lewis BA, Freyssinet JM, Bock PE, Shore JD
(1977) Biochem Soc Trans 5: 1247-51
MeSH Terms: Calcium, Enzyme Activation, Factor XIII, Fibrin, Macromolecular Substances, Protein Binding, Spectrometry, Fluorescence, Thrombin
Added January 20, 2015
0 Communities
1 Members
0 Resources
8 MeSH Terms
Interaction of bovine platelets with bovine glomerular basement membrane.
Davis JL, Chandler DK, Riquetti P, Rogers S, Kocan KM, Ownby CL, Hudson BG, Carraway KL
(1979) Thromb Haemost 42: 1217-29
MeSH Terms: Animals, Basement Membrane, Blood Platelets, Calcium, Cattle, Female, Kidney Glomerulus, Platelet Adhesiveness, Platelet Aggregation, Serotonin
Show Abstract · Added December 10, 2013
The interaction of bovine platelets with bovine glomerular basement membrane has been studied by aggregometry, transmission and scanning electron microscopy and measurement of [3H] serotonin release. In the absence of added calcium platelets adhere to basement membrane but fail to undergo the release reaction or aggregation. In the presence of 0.2-0.5 mM calcium release of serotonin and complete aggregation of the platelets are observed when sufficient basement membrane is present. Platelets were strongly adhered to the basement membrane surface, the platelet surface in the aggregates closely following the surface of the basement membrane. Platelet morphology in aggregates with basement membrane closely resembled that of platelets from collagen-induced aggregates. Basement membrane differed from collagen in its requirement for calcium for the aggregation and release reactions. In addition purified basement membrane was 1.5-3 fold less active on a weight basis than bovine tendon collagen in promoting aggregation.
1 Communities
1 Members
0 Resources
10 MeSH Terms
Severe hypercalcemia associated with hydrochlorothiazide and calcium carbonate therapy.
Hakim R, Tolis G, Goltzman D, Meltzer S, Friedman R
(1979) Can Med Assoc J 121: 591-4
MeSH Terms: Aged, Antacids, Calcium Carbonate, Diagnosis, Differential, Female, Furosemide, Hormones, Humans, Hydrochlorothiazide, Hydrocortisone, Hypercalcemia, Hyperparathyroidism, Potassium Chloride, Sodium Chloride
Added May 20, 2014
0 Communities
1 Members
0 Resources
14 MeSH Terms
Calcium-dependent stimulation of thromboxane and prostaglandin biosynthesis by ionophores.
Oelz O, Knapp HR, Roberts LJ, Oelz R, Sweetman BJ, Oates JA, Reed PW
(1978) Adv Prostaglandin Thromboxane Res 3: 147-58
MeSH Terms: Blood Platelets, Calcimycin, Calcium, Indomethacin, Ionophores, Kidney, Lasalocid, Oxygen Consumption, Platelet Aggregation, Prostaglandins, Thromboxanes
Added December 10, 2013
0 Communities
1 Members
0 Resources
11 MeSH Terms
Primary hemostasis (collagen-platelet interaction): introduction.
Cunningham LW, Santoro S, Davies H
(1978) Suppl Thromb Haemost 63: 205-13
MeSH Terms: Animals, Blood Platelets, Calcium, Collagen, Hemostasis, Humans, Platelet Aggregation, Rats, Skin Physiological Phenomena, Wound Healing
Added March 5, 2014
1 Communities
1 Members
0 Resources
10 MeSH Terms
Mechanisms of catecholamine actions on liver carbohydrate metabolism.
Exton JH, Assimacopoulos-Jeannet FD, Blackmore PF, Cherrington AD, Chan TM
(1978) Adv Cyclic Nucleotide Res 9: 441-52
MeSH Terms: Animals, Calcimycin, Calcium, Carbohydrate Metabolism, Catecholamines, Cyclic AMP, Enzyme Activation, Epinephrine, In Vitro Techniques, Liver, Liver Glycogen, Phenylephrine, Phosphorylases, Protein Kinases, Rats, Sympatholytics
Show Abstract · Added December 10, 2013
Epinephrine rapidly activates phosphorylase in hepatocytes, mainly by a mechanism(s) involving alpha-adrenergic and not beta-adrenergic receptors. The alpha-adrenergic mechanism does not involve accumulation of cAMP or activation of cAMP-dependent protein kinase. It is impaired when hepatocytes are depleted of calcium by EGTA treatment and is rapidly restored by readdition of calcium. Basal phosphorylase is also lowered by calcium deficiency and rapidly increased by calcium but not other divalent cations. The divalent cation ioniphore A23187 increases phosphorylase a levels in hepatocytes in a calcium-dependent manner. Calcium deficiency does not modify the effects of glucagon, cAMP, or beta-adrenergic activation on phosphorylase. Activation of alpha-adrenergic receptors rapidly increases 45Ca fluxes in hepatocytes. Glucagon produces similar effects, but supraphysiological concentrations are required. The hypothesis is advanced that alpha-adrenergic activation of phosphorylase involves alterations in cell calcium such that there is an increase in cytosolic Ca2+ concentration leading to increased phosphorylase kinase activity. Epinephrine induces greater cAMP accumulation in calcium-depleted cells than in normal cells. The effect is mediated by alpha-adrenergic and not beta-adrenergic receptors. Calcium deficiency also cuases cAMP accumulation in hepatocytes incubated with phenylephrine but does not modify the responses of the cells to isoproterenol, glucagon, or cAMP. Low concentrations of calcium rapidly reverse alpha-adrenergic receptor-mediated cAMP accumulation in calcium-depleted cells. The hypothesis is advanced that calcium normally exerts an inhibitory effect on a linkage between alpha-adrenergic receptors and adenylate cyclase in hepatocytes.
0 Communities
1 Members
0 Resources
16 MeSH Terms
Calcium and the assembly of human blood-clotting factor XIII.
Bock PE, Beavis AD, Holbrook JJ
(1979) Biochem Soc Trans 7: 627-8
MeSH Terms: Calcium, Edetic Acid, Factor XIII, Humans, Macromolecular Substances, Molecular Weight, Thrombin
Added January 20, 2015
0 Communities
1 Members
0 Resources
7 MeSH Terms
The composition and calcium transport activity of the sarcoplasmic reticulum from goats with and without heritable myotonia.
Swift LL, Atkinson JB, LeQuire VS
(1979) Lab Invest 40: 384-90
MeSH Terms: Animals, Biological Transport, Calcium, Electromyography, Goats, Haplorhini, Lipid Metabolism, Muscle Proteins, Myotonia, Sarcoplasmic Reticulum, Sialic Acids, Time Factors
Show Abstract · Added May 27, 2014
Studies were conducted on purified sarcoplasmic reticulum isolated from myotonic goats, an animal model of heritable myotonia. When compared to sarcoplasmic reticulum from normal goats, fragmented sarcoplasmic reticulum from the myotonic goat had (1) increased levels of calcium, (2) increased rates of calcium uptake and efflux, (3) an increased sialic acid content, and (4) an increased content of saturated fatty acids. These differences support the concept of a structural and functional defect as a basis for the abnormal contraction-relaxation characteristics of myotonia.
0 Communities
1 Members
0 Resources
12 MeSH Terms