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Human immune system development and rejection of human islet allografts in spontaneously diabetic NOD-Rag1null IL2rgammanull Ins2Akita mice.
Brehm MA, Bortell R, Diiorio P, Leif J, Laning J, Cuthbert A, Yang C, Herlihy M, Burzenski L, Gott B, Foreman O, Powers AC, Greiner DL, Shultz LD
(2010) Diabetes 59: 2265-70
MeSH Terms: Adaptive Immunity, Animals, Blood Glucose, CD4-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes, Crosses, Genetic, Flow Cytometry, Humans, Immunity, Innate, Interleukin Receptor Common gamma Subunit, Islets of Langerhans Transplantation, Mice, Mice, Inbred BALB C, Mice, Inbred NOD, Mice, SCID, Mutation, Transplantation, Heterologous, Transplantation, Homologous
Show Abstract · Added December 5, 2013
OBJECTIVE - To create an immunodeficient mouse model that spontaneously develops hyperglycemia to serve as a diabetic host for human islets and stem cell-derived beta-cells in the absence or presence of a functional human immune system.
RESEARCH DESIGN AND METHODS - We backcrossed the Ins2(Akita) mutation onto the NOD-Rag1(null) IL2rgamma(null) strain and determined 1) the spontaneous development of hyperglycemia, 2) the ability of human islets, mouse islets, and dissociated mouse islet cells to restore euglycemia, 3) the generation of a human immune system following engraftment of human hematopoietic stem cells, and 4) the ability of the humanized mice to reject human islet allografts.
RESULTS - We confirmed the defects in innate and adaptive immunity and the spontaneous development of hyperglycemia conferred by the IL2rgamma(null), Rag1(null), and Ins2(Akita) genes in NOD-Rag1(null) IL2rgamma(null) Ins2(Akita) (NRG-Akita) mice. Mouse and human islets restored NRG-Akita mice to normoglycemia. Insulin-positive cells in dissociated mouse islets, required to restore euglycemia in chemically diabetic NOD-scid IL2rgamma(null) and spontaneously diabetic NRG-Akita mice, were quantified following transplantation via the intrapancreatic and subrenal routes. Engraftment of human hematopoietic stem cells in newborn NRG-Akita and NRG mice resulted in equivalent human immune system development in a normoglycemic or chronically hyperglycemic environment, with >50% of engrafted NRG-Akita mice capable of rejecting human islet allografts.
CONCLUSIONS - NRG-Akita mice provide a model system for validation of the function of human islets and human adult stem cell, embryonic stem cell, or induced pluripotent stem cell-derived beta-cells in the absence or presence of an alloreactive human immune system.
1 Communities
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18 MeSH Terms
Natural killer T cells and atherosclerosis: form and function meet pathogenesis.
Braun NA, Covarrubias R, Major AS
(2010) J Innate Immun 2: 316-24
MeSH Terms: Adaptive Immunity, Animals, Arteriosclerosis, Chronic Disease, Cytokines, Dyslipidemias, Glycolipids, Humans, Immunity, Innate, Inflammation, Mice, Natural Killer T-Cells
Show Abstract · Added February 11, 2014
Atherosclerosis is a chronic inflammatory disease characterized by dyslipidemia and accumulation of lipids in the arterial intima, with activation of both innate and adaptive immunity. Reciprocally, dyslipidemia associated with atherosclerosis can perturb normal immune function. Natural killer T (NKT) cells are a specialized group of immune cells that share characteristics with both conventional T cells and natural killer cells. However, unlike these cells, NKT cells recognize glycolipid antigens and produce both pro- and anti-inflammatory cytokines upon activation. Because of these unique characteristics, NKT cells have recently been ascribed a role in the regulation of immunity and inflammation, including cardiovascular disease. In addition, NKT cells represent a bridge between dyslipidemia and immune regulation. This review summarizes the current knowledge of NKT cells and discusses the interplay between dyslipidemia and the normal functions of NKT cells and how this might modulate inflammation and atherosclerosis.
(c) 2010 S. Karger AG, Basel.
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12 MeSH Terms
Adaptive interactions between HLA and HIV-1: highly divergent selection imposed by HLA class I molecules with common supertype motifs.
John M, Heckerman D, James I, Park LP, Carlson JM, Chopra A, Gaudieri S, Nolan D, Haas DW, Riddler SA, Haubrich R, Mallal S
(2010) J Immunol 184: 4368-77
MeSH Terms: Adaptive Immunity, Amino Acid Motifs, Cohort Studies, Genetic Variation, Genotype, HIV Infections, HIV-1, HLA Antigens, Histocompatibility Antigens Class I, Histocompatibility Testing, Humans, Molecular Sequence Data, Peptides, Polymorphism, Genetic, Protein Binding, Randomized Controlled Trials as Topic, Receptors, Antigen, T-Cell, United States, Western Australia
Show Abstract · Added March 13, 2015
Currently, 1.1 million individuals in the United States are living with HIV-1 infection. Although this is a relatively small proportion of the global pandemic, the remarkable mix of ancestries in the United States, drawn together over the past two centuries of continuous population migrations, provides an important and unique perspective on adaptive interactions between HIV-1 and human genetic diversity. HIV-1 is a rapidly adaptable organism and mutates within or near immune epitopes that are determined by the HLA class I genotype of the infected host. We characterized HLA-associated polymorphisms across the full HIV-1 proteome in a large, ethnically diverse national United States cohort of HIV-1-infected individuals. We found a striking divergence in the immunoselection patterns associated with HLA variants that have very similar or identical peptide-binding specificities but are differentially distributed among racial/ethnic groups. Although their similarity in peptide binding functionally clusters these HLA variants into supertypes, their differences at sites within the peptide-binding groove contribute to race-specific selection effects on circulating HIV-1 viruses. This suggests that the interactions between the HLA/HIV peptide complex and the TCR vary significantly within HLA supertype groups, which, in turn, influences HIV-1 evolution.
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19 MeSH Terms
An unexpected counter-regulatory role of IL-10 in B-lymphocyte-mediated transplantation tolerance.
Zhao G, Moore DJ, Lee KM, Kim JI, Duff PE, O'Connor MR, Hirohashi T, Lei J, Yang M, Markmann JF, Deng S
(2010) Am J Transplant 10: 796-801
MeSH Terms: Adaptive Immunity, Animals, B-Lymphocytes, Graft Survival, Interleukin-10, Mice, Mice, Inbred C3H, Mice, Inbred C57BL, Neutralization Tests, Transplantation Immunology
Show Abstract · Added October 24, 2013
Monoclonal antibody against the CD45RB protein induces stable transplantation tolerance to multiple types of allograft. We have previously established that this tolerance protocol relies on the regulatory function of B lymphocytes for its effect. B lymphocytes have also been reported to participate in immune regulation in several other settings. In most of these systems, the regulatory function of B lymphocytes depends on the production of IL-10. Therefore, we investigated the role of IL-10 in the anti-CD45RB model of B-cell-mediated transplantation tolerance. Surprisingly, using antibody-mediated neutralization of IL-10, IL-10-deficient recipients and adoptive transfer of IL-10-deficient B lymphocytes, we found that IL-10 actually counter-regulates tolerance induced by anti-CD45RB. Furthermore, neutralization of IL-10 reduced the development of chronic allograft vasculopathy compared to anti-CD45RB alone and reduced the production of graft reactive alloantibodies. These data suggest that the participation of regulatory B lymphocytes in transplantation tolerance may be distinct from how they operate in other systems. Identifying the specific B lymphocytes that mediate transplantation tolerance and defining their mechanism of action may yield new insights into the complex cellular network through which antigen-specific tolerance is established and maintained.
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10 MeSH Terms
Role of the adaptive immune system in hypertension.
Harrison DG, Vinh A, Lob H, Madhur MS
(2010) Curr Opin Pharmacol 10: 203-7
MeSH Terms: Adaptive Immunity, Animals, Antigens, Humans, Hypertension, Models, Immunological, T-Lymphocytes
Show Abstract · Added December 10, 2013
Recent studies have shown that both innate and adaptive immunity contribute to hypertension. Inflammatory cells, including macrophages and T cells accumulate in the vessel wall, particularly in the perivascular fat, and in the kidney of hypertensive animals. Mice lacking lymphocytes are resistant to the development of hypertension, and adoptive transfer of T cells restores hypertensive responses to angiotensin II and DOCA-salt challenge. Immune modulating agents have variable, but often-beneficial effects in ameliorating end-organ damage and blood pressure elevation in experimental hypertension. The mechanisms by which hypertension stimulates an immune response remain unclear, but might involve the formation of neoantigens that activate adaptive immunity. Identification of these neoantigens and understanding how they form might prove useful in the prevention and treatment of this widespread and devastating disease.
Copyright 2010 Elsevier Ltd. All rights reserved.
2 Communities
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7 MeSH Terms
Immunoglobulin superfamily virus receptors and the evolution of adaptive immunity.
Dermody TS, Kirchner E, Guglielmi KM, Stehle T
(2009) PLoS Pathog 5: e1000481
MeSH Terms: Adaptive Immunity, Animals, Evolution, Molecular, Humans, Immunoglobulins, Ligands, Receptors, Virus
Added December 10, 2013
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7 MeSH Terms
Th1 and Th17 cells induce proliferative glomerulonephritis.
Summers SA, Steinmetz OM, Li M, Kausman JY, Semple T, Edgtton KL, Borza DB, Braley H, Holdsworth SR, Kitching AR
(2009) J Am Soc Nephrol 20: 2518-24
MeSH Terms: Adaptive Immunity, Adoptive Transfer, Animals, Chemokines, Disease Models, Animal, Genes, RAG-1, Glomerulonephritis, Membranoproliferative, Immunoglobulin G, Interleukin-17, Mice, Mice, Inbred C57BL, Mice, Knockout, Ovalbumin, RNA, Messenger, T-Lymphocyte Subsets, T-Lymphocytes, Helper-Inducer, Th1 Cells
Show Abstract · Added August 21, 2013
Th1 effector CD4+ cells contribute to the pathogenesis of proliferative and crescentic glomerulonephritis, but whether effector Th17 cells also contribute is unknown. We compared the involvement of Th1 and Th17 cells in a mouse model of antigen-specific glomerulonephritis in which effector CD4+ cells are the only components of adaptive immunity that induce injury. We planted the antigen ovalbumin on the glomerular basement membrane of Rag1(-/-) mice using an ovalbumin-conjugated non-nephritogenic IgG1 monoclonal antibody against alpha3(IV) collagen. Subsequent injection of either Th1- or Th17-polarized ovalbumin-specific CD4+ effector cells induced proliferative glomerulonephritis. Mice injected with Th1 cells developed progressive albuminuria over 21 d, histologic injury including 5.5 +/- 0.9% crescent formation/segmental necrosis, elevated urinary nitrate, and increased renal NOS2, CCL2, and CCL5 mRNA. Mice injected with Th17 cells developed albuminuria by 3 d; compared with Th1-injected mice, their glomeruli contained more neutrophils and greater expression of renal CXCL1 mRNA. In conclusion, Th1 and Th17 effector cells can induce glomerular injury. Understanding how these two subsets mediate proliferative forms of glomerulonephritis may lead to targeted therapies.
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17 MeSH Terms
Fms-like tyrosine kinase-3 ligand alters antigen-specific responses to infections after severe burn injury.
Bohannon J, Fang G, Cui W, Sherwood E, Toliver-Kinsky T
(2009) Shock 32: 435-41
MeSH Terms: Adaptive Immunity, Animals, Burns, Dendritic Cells, Immunoglobulin A, Immunoglobulin G, Immunoglobulin M, Interferon-gamma, Interleukin-10, Interleukin-12, Male, Membrane Proteins, Mice, Mice, Inbred BALB C, Pseudomonas Infections, Pseudomonas aeruginosa
Show Abstract · Added October 18, 2015
Burn patients are susceptible to opportunistic infections partly because of decreased immune functions, especially TH1-driven antigen-specific responses, which are regulated by dendritic cells. The dendritic cell growth factor, fms-like tyrosine kinase-3 ligand (FL), has been shown to increase resistance to Pseudomonas aeruginosa, in a dendritic cell-dependent manner, in a mouse model of burn wound infection. The specific mechanisms of protection are not known. This study tested the hypothesis that FL can enhance production of P. aeruginosa-specific antibodies after burn wound infection. Mice that had been previously exposed to P. aeruginosa were infected after burn injury by wound inoculation or challenged by intraperitoneal injection of heat-killed P. aeruginosa. In response to wound infection, FL treatments enhanced bacterial clearance and induced a shift from immunoglobulin (Ig) M toward IgG and IgA. However, serum levels of neither P. aeruginosa-specific antibodies nor interferon gamma (IFN-gamma) were significantly increased by FL, possibly because of decreased systemic exposure to bacteria. After challenge with heat-killed bacteria, which ensured equal exposures, FL-treated mice produced significantly greater levels of P. aeruginosa-specific IgG2a, which correlated with an increase in serum levels of interferon gamma and enhanced opsonization capacity. IL-12, IL-10, and transforming growth factor beta were significantly increased in FL-treated mice, regardless of the type of challenge. These findings indicate that FL treatments after burn injury enhance cytokine responses to recall antigens and increase bacterial clearance. In addition, through its ability to promote TH1-associated antigen-specific responses, FL may have potential as an immunotherapy to enhance adaptive immunity after severe burn injury.
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16 MeSH Terms