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Results: 31 to 33 of 33

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Embryonic germ cell lines and their derivation from mouse primordial germ cells.
Labosky PA, Barlow DP, Hogan BL
(1994) Ciba Found Symp 182: 157-68; discussion 168-78
MeSH Terms: Animals, Blastocyst, Cell Differentiation, Cell Line, Cell Transplantation, Chimera, Female, Genomic Imprinting, Germ Cells, Growth Substances, Hair Color, Male, Mice, Mice, Inbred Strains, Mice, Nude, Receptor, IGF Type 2, Stem Cells, Teratocarcinoma
Show Abstract · Added July 20, 2010
When primordial germ cells of the mouse are cultured on feeder layers with the addition of the polypeptide signalling molecules leukaemia inhibitory factor, Steel factor and basic fibroblast growth factor they give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells. These primordial germ cell-derived embryonic germ cells (EG cells) can be induced to differentiate extensively in culture and also form teratocarcinomas when injected into nude mice. Additionally, they contribute to chimeras when injected into host blastocysts. We have derived multiple EG cell lines from 8.5 days post coitum (dpc) embryos of C57BL/6 inbred mice. Four independent EG cell lines with normal male karyotypes have formed chimeras (up to 70% coat colour chimerism) when injected into BALB/c host blastocysts. Chimeric mice from all four cell lines are fertile, but only those from one line have transmitted coat colour markers through the germline. Studies have also been carried out to determine whether gonadal primordial germ cells can give rise to pluripotent EG cells. Germ cells from gonads of 15.5 dpc C57BL/6 embryos and newborn mice failed to produce EG cell lines. EG cell lines capable of forming teratocarcinomas and coat colour chimeras have been established from primordial germ cells of 12.5 dpc genital ridges. We are currently testing the genomic imprinting status of the insulin-like growth factor type 2 receptor gene (Igf2r) in our different EG cell lines.
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18 MeSH Terms
Mouse embryonic germ (EG) cell lines: transmission through the germline and differences in the methylation imprint of insulin-like growth factor 2 receptor (Igf2r) gene compared with embryonic stem (ES) cell lines.
Labosky PA, Barlow DP, Hogan BL
(1994) Development 120: 3197-204
MeSH Terms: Animals, Base Sequence, Blotting, Southern, Cell Differentiation, Cell Line, DNA Primers, Genes, Genomic Imprinting, Germ Cells, Methylation, Mice, Mice, Inbred Strains, Mice, Transgenic, Molecular Sequence Data, Polymerase Chain Reaction, Receptor, IGF Type 2, Stem Cells
Show Abstract · Added July 20, 2010
Primordial germ cells of the mouse cultured on feeder layers with leukemia inhibitory factor, Steel factor and basic fibroblast growth factor give rise to cells that resemble undifferentiated blastocyst-derived embryonic stem cells. These primordial germ cell-derived embryonic germ cells can be induced to differentiate extensively in culture, form teratocarcinomas when injected into nude mice and contribute to chimeras when injected into host blastocysts. Here, we report the derivation of multiple embryonic germ cell lines from 8.5 days post coitum embryos of C57BL/6 inbred mice. Four independent embryonic germ cell lines with normal male karyotypes have formed chimeras when injected into BALB/c host blastocysts and two of these lines have transmitted coat color markers through the germline. We also show that pluripotent cell lines capable of forming teratocarcinomas and coat color chimeras can be established from primordial germ cells of 8.0 days p.c. embryos and 12.5 days p.c. genital ridges. We have examined the methylation status of the putative imprinting box of the insulin-like growth factor type 2 receptor gene (Igf2r) in these embryonic germ cell lines. No correlation was found between methylation pattern and germline competence. A significant difference was observed between embryonic stem cell and embryonic germ cell lines in their ability to maintain the methylation imprint of the Igf2r gene in culture. This may illustrate a fundamental difference between these two cell types.
1 Communities
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17 MeSH Terms
Tissue-specific and allele-specific replication timing control in the imprinted human Prader-Willi syndrome region.
Gunaratne PH, Nakao M, Ledbetter DH, Sutcliffe JS, Chinault AC
(1995) Genes Dev 9: 808-20
MeSH Terms: Cells, Cultured, Chromosomes, Human, Pair 15, DNA Replication, Female, Genomic Imprinting, Humans, Lymphocytes, Male, Neurons, Prader-Willi Syndrome, Ribonucleoproteins, Small Nuclear, Sequence Deletion, Transcription, Genetic, Tumor Cells, Cultured
Show Abstract · Added February 20, 2014
To examine the relationship between replication timing and differential gene transcription in tissue-specific and imprinted settings we have studied the replication timing properties of the human Prader-Willi syndrome (PWS) region on human chromosome 15q11-13. Interphase fluorescence in situ hybridization with an overlapping series of cosmid clones was used to map a PWS replication timing domain to a 500- to 650-kb region that includes the SNRPN gene. This PWS domain replicates late in lymphocytes but predominantly early in neuroblasts, with replication asynchrony observed in both tissues, and appears to colocalize with a genetically imprinted transcription domain showing prominent expression in the brain. A 5- to 30-kb deletion in the 5' region of SNRPN results in the loss of late replication control of this domain in lymphocytes when the deleted chromosome is inherited paternally. This potential allele-specific replication timing control region also appears to colocalize with a putative imprinting control region that has been shown previously to abolish the expression of three imprinted transcripts in this same region.
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14 MeSH Terms