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Evidence for repatterning of the gastric fundic epithelium associated with Ménétrier's disease and TGFalpha overexpression.
Nomura S, Settle SH, Leys CM, Means AL, Peek RM, Leach SD, Wright CV, Coffey RJ, Goldenring JR
(2005) Gastroenterology 128: 1292-305
MeSH Terms: Animals, Atrophy, Epithelium, Gastric Fundus, Gastrins, Gastritis, Hypertrophic, Gene Expression, Homeodomain Proteins, Hyperplasia, Mice, Mice, Transgenic, Mucins, Muscle Proteins, Parietal Cells, Gastric, Peptides, Trans-Activators, Transforming Growth Factor alpha, Trefoil Factor-2
Show Abstract · Added October 7, 2013
BACKGROUND & AIMS - Increase of intramucosal transforming growth factor alpha (TGFalpha) levels in the gastric fundus leads to oxyntic atrophy and massive foveolar hyperplasia in both metallothionein (MT)-TGFalpha mice and patients with Ménétrier's disease. We have evaluated the hypothesis that increased levels of TGFalpha in the fundus induces an antral pattern of cell differentiation in fundic glands by studying Pdx1, a transcription factor whose expression normally is confined to the gastric antrum.
METHODS - Induction of Pdx1 expression was evaluated in Pdx1(lacZ/+)/MT-TGFalpha bigenic mice treated with zinc. The distribution of Pdx1 in MT-TGFalpha mice and Ménétrier's disease patients was evaluated with anti-Pdx1 antibodies. Transcript levels were evaluated by quantitative polymerase chain reaction in mouse and human tissues and AGS cells.
RESULTS - In Pdx1(lacZ/+) mice, Pdx1 was expressed in antral mucosal cells including gastrin cells and TFF2-expressing deep glandular mucous cells. Zinc treatment for 2 to 8 weeks in Pdx1(lacZ/+)/MT-TGFalpha transgenic mice resulted in expression of Pdx1 throughout the fundus. No ectopic fundic Pdx1 expression was observed in either H. felis-infected or DMP777-treated mice. In MT-TGFalpha mice, 8 weeks of zinc treatment elicited nuclear Pdx1 staining throughout the fundic mucosa. TGFalpha treatment in AGS cells led to increases in Pdx1 and gastrin messenger RNA expression. Fundic sections from Ménétrier's disease patients showed nuclear Pdx1 staining throughout the fundic glands. Treatment of a Ménétrier's disease patient with an anti-epidermal growth factor receptor monoclonal antibody reduced fundic expression of both Pdx1 and gastrin.
CONCLUSIONS - Overexpression of TGFalpha in MT-TGFalpha mice and Ménétrier's disease patients elicits ectopic expression in the fundus of Pdx1, consistent with the phenotype of antralization.
3 Communities
5 Members
0 Resources
18 MeSH Terms
Alterations in gastric mucosal lineages induced by acute oxyntic atrophy in wild-type and gastrin-deficient mice.
Nomura S, Yamaguchi H, Ogawa M, Wang TC, Lee JR, Goldenring JR
(2005) Am J Physiol Gastrointest Liver Physiol 288: G362-75
MeSH Terms: Animals, Atrophy, Azetidines, Biological Transport, Cell Lineage, Gastric Mucosa, Gastrins, Gene Expression, Intrinsic Factor, Mice, Mice, Inbred C57BL, Mice, Knockout, Mucins, Muscle Proteins, Parietal Cells, Gastric, Peptides, Piperazines, Trefoil Factor-2
Show Abstract · Added October 7, 2013
In addition to their role in gastric acid secretion, parietal cells secrete a number of growth factors that may influence the differentiation of other gastric lineages. Indeed, oxyntic atrophy is considered the most significant correlate with increased risk for gastric adenocarcinoma. We studied the alterations in gastric mucosal lineages elicited by acute oxyntic atrophy induced by treatment of C57BL/6 and gastrin-deficient mice with the parietal cell protonophore [S-(R*,S*)]-N-[1-(1,3-benzodioxol-5-yl)butyl]-3,3-diethyl-2-[4-[(4-methyl-1-piperazinyl)carbonyl]phenoxy]-4-oxo-1-azetidinecarboxamide (DMP-777). In both wild-type and gastrin knockout mice, DMP-777 elicited the rapid loss of parietal cells within 2 days of treatment. In wild-type mice, oxyntic atrophy was accompanied by a rapid increase in 5-bromo-2'-deoxyuridine-labeled proliferative cells and attendant increase in surface cell numbers. However, gastrin knockout mice did not demonstrate significant foveolar hyperplasia and showed a blunted proliferative response. After 7 days of treatment in wild-type mice, a second proliferative population emerged at the base of fundic glands along with the development of a mucous cell metaplasia expressing TFF2/spasmolytic polypeptide (SPEM). However, in gastrin knockout mice, SPEM expressing both TFF2 mRNA and protein developed after only 1 day of DMP-777 treatment. In wild-type mice, all changes induced by DMP-777 were reversed 14 days after cessation of treatment. In gastrin-deficient mice, significant SPEM was still present 14 days after the cessation of treatment. The results indicate that foveolar hyperplasia requires the influence of gastrin, whereas SPEM develops in response to oxyntic atrophy independent of gastrin, likely through transdifferentiation of chief cells.
1 Communities
1 Members
0 Resources
18 MeSH Terms
Regulation and function of COX-2 gene expression in isolated gastric parietal cells.
Pausawasdi N, Ramamoorthy S, Crofford LJ, Askari FK, Todisco A
(2002) Am J Physiol Gastrointest Liver Physiol 282: G1069-78
MeSH Terms: Animals, Carbachol, Cells, Cultured, Cholinergic Agonists, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Cyclooxygenase Inhibitors, Dogs, Enzyme Inhibitors, Gene Expression Regulation, Enzymologic, Histamine, Imidazoles, Indoles, Isoenzymes, Maleimides, NF-kappa B, Nitrobenzenes, Parietal Cells, Gastric, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Pyridines, Sulfonamides
Show Abstract · Added September 18, 2013
We examined expression, function, and regulation of the cyclooxygenase (COX)-2 gene in gastric parietal cells. COX-2-specific mRNA was isolated from purified (>95%) canine gastric parietal cells in primary culture and measured by Northern blots using a human COX-2 cDNA probe. Carbachol was the most potent inducer of COX-2 gene expression. Gastrin and histamine exhibited minor stimulatory effects. Carbachol-stimulated expression was inhibited by intracellular Ca(2+) chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-AM (90%), protein kinase C (PKC) inhibitor GF-109203X (48%), and p38 kinase inhibitor SB-203580 (48%). Nuclear factor (NF)-kappaB inhibitor 1-pyrrolidinecarbodithioic acid inhibited carbachol-stimulated expression by 80%. Similar results were observed in the presence of adenoviral vector Ad.dom.neg.IkappaB, which expresses a repressor of NF-kappaB. Addition of SB-203580 with Ad.dom.neg.IkappaB almost completely blocked carbachol stimulation of COX-2 gene expression. We examined the effect of carbachol on PGE(2) release by enzyme-linked immunoassay. Carbachol induced PGE(2) release. Ad.dom.neg.IkappaB, alone or with SB-203580, produced, respectively, partial (70%) and almost complete (>80%) inhibition of carbachol-stimulated PGE(2) production. Selective COX-2 inhibitor NS-398 blocked carbachol-stimulated PGE(2) release without affecting basal PGE(2) production. In contrast, indomethacin inhibited both basal and carbachol-stimulated PGE(2) release. Carbachol induces COX-2 gene expression in the parietal cells through signaling pathways that involve intracellular Ca(2+), PKC, p38 kinase, and activation of NF-kappaB. The functional significance of these effects seems to be stimulation of PGE(2) release.
0 Communities
1 Members
0 Resources
22 MeSH Terms
Treatment of Ménétrier's disease with a monoclonal antibody against the epidermal growth factor receptor.
Burdick JS, Chung E, Tanner G, Sun M, Paciga JE, Cheng JQ, Washington K, Goldenring JR, Coffey RJ
(2000) N Engl J Med 343: 1697-701
MeSH Terms: Anaphylaxis, Antibodies, Monoclonal, Contrast Media, ErbB Receptors, Fatal Outcome, Gastric Mucosa, Gastritis, Hypertrophic, Heart Arrest, Humans, Hypertension, Pulmonary, Male, Middle Aged, Parietal Cells, Gastric, Precancerous Conditions, Vomiting
Added April 12, 2016
0 Communities
1 Members
0 Resources
15 MeSH Terms