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Temporal self-regulation of transposition through host-independent transposase rodlet formation.
Woodard LE, Downes LM, Lee YC, Kaja A, Terefe ES, Wilson MH
(2017) Nucleic Acids Res 45: 353-366
MeSH Terms: Animals, DNA Transposable Elements, Female, Gene Expression Regulation, Genes, Reporter, HEK293 Cells, HeLa Cells, Humans, Insect Proteins, Luciferases, Male, Mice, Optical Imaging, Time-Lapse Imaging, Transposases, Tribolium
Added December 8, 2017
0 Communities
2 Members
0 Resources
16 MeSH Terms
Bacterial Hypoxic Responses Revealed as Critical Determinants of the Host-Pathogen Outcome by TnSeq Analysis of Staphylococcus aureus Invasive Infection.
Wilde AD, Snyder DJ, Putnam NE, Valentino MD, Hammer ND, Lonergan ZR, Hinger SA, Aysanoa EE, Blanchard C, Dunman PM, Wasserman GA, Chen J, Shopsin B, Gilmore MS, Skaar EP, Cassat JE
(2015) PLoS Pathog 11: e1005341
MeSH Terms: Animals, Cell Hypoxia, Cell Line, DNA Transposable Elements, Disease Models, Animal, Female, Gene Expression Regulation, Bacterial, Genes, Viral, Host-Pathogen Interactions, Humans, Mice, Mice, Inbred C57BL, Oligonucleotide Array Sequence Analysis, Osteomyelitis, Quorum Sensing, Reverse Transcriptase Polymerase Chain Reaction, Staphylococcal Infections, Staphylococcus aureus, Virulence, Virulence Factors
Added February 8, 2016
0 Communities
2 Members
0 Resources
20 MeSH Terms
Anti-Tumor Effects after Adoptive Transfer of IL-12 Transposon-Modified Murine Splenocytes in the OT-I-Melanoma Mouse Model.
Galvan DL, O'Neil RT, Foster AE, Huye L, Bear A, Rooney CM, Wilson MH
(2015) PLoS One 10: e0140744
MeSH Terms: Adoptive Transfer, Animals, DNA Transposable Elements, HeLa Cells, Humans, Interleukin-12, Melanoma, Mice, Mice, Inbred NOD, Mice, SCID, Neoplasms, Experimental, Spleen, T-Lymphocytes
Added March 7, 2016
0 Communities
1 Members
0 Resources
13 MeSH Terms
Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells.
Saha S, Woodard LE, Charron EM, Welch RC, Rooney CM, Wilson MH
(2015) Nucleic Acids Res 43: 1770-82
MeSH Terms: 5' Untranslated Regions, Chromosomes, Artificial, Bacterial, DNA Damage, DNA Transposable Elements, Genome, Human, Green Fluorescent Proteins, HEK293 Cells, Humans, Plasmids, Polymerase Chain Reaction, Transgenes, Transposases
Added January 30, 2015
0 Communities
2 Members
0 Resources
12 MeSH Terms
Glycolytic enzymes localize to ribonucleoprotein granules in Drosophila germ cells, bind Tudor and protect from transposable elements.
Gao M, Thomson TC, Creed TM, Tu S, Loganathan SN, Jackson CA, McCluskey P, Lin Y, Collier SE, Weng Z, Lasko P, Ohi MD, Arkov AL
(2015) EMBO Rep 16: 379-86
MeSH Terms: Animals, Animals, Genetically Modified, Base Sequence, Cytoplasmic Granules, DNA Transposable Elements, Drosophila, Drosophila Proteins, Germ Cells, Glycolysis, Membrane Transport Proteins, MicroRNAs, Molecular Sequence Data, Ribonucleoproteins, Sequence Analysis, DNA
Added January 5, 2016
1 Communities
3 Members
0 Resources
14 MeSH Terms
Anti-leukemic potency of piggyBac-mediated CD19-specific T cells against refractory Philadelphia chromosome-positive acute lymphoblastic leukemia.
Saito S, Nakazawa Y, Sueki A, Matsuda K, Tanaka M, Yanagisawa R, Maeda Y, Sato Y, Okabe S, Inukai T, Sugita K, Wilson MH, Rooney CM, Koike K
(2014) Cytotherapy 16: 1257-69
MeSH Terms: Antigens, CD19, Cancer Vaccines, Cell Line, Tumor, Cell Proliferation, Culture Media, Serum-Free, Cytotoxicity, Immunologic, DNA Transposable Elements, Drug Resistance, Neoplasm, Genetic Engineering, Genetic Vectors, Humans, Immunotherapy, Adoptive, Interleukin-15, Interleukin-2, Leukemia, Myelogenous, Chronic, BCR-ABL Positive, Mutation, Protein Kinase Inhibitors, Receptors, Antigen, T-Cell, Recombinant Fusion Proteins, T-Lymphocytes, TNF-Related Apoptosis-Inducing Ligand, Up-Regulation
Added October 28, 2014
0 Communities
1 Members
0 Resources
22 MeSH Terms
A piggyBac insertion disrupts Foxl2 expression that mimics BPES syndrome in mice.
Shi F, Ding S, Zhao S, Han M, Zhuang Y, Xu T, Wu X
(2014) Hum Mol Genet 23: 3792-800
MeSH Terms: Animals, Blepharophimosis, DNA Transposable Elements, Disease Models, Animal, Forkhead Box Protein L2, Forkhead Transcription Factors, Humans, Maxilla, Mice, Mutagenesis, Insertional, Skin Abnormalities, Urogenital Abnormalities
Added September 26, 2018
0 Communities
1 Members
0 Resources
MeSH Terms
Targeting piggyBac transposon integrations in the human genome.
Galvan DL, Kettlun CS, Wilson MH
(2014) Methods Mol Biol 1114: 143-61
MeSH Terms: DNA Transposable Elements, Gene Order, Gene Targeting, Genetic Vectors, Genome, Human, HEK293 Cells, Homologous Recombination, Humans, Mutagenesis, Insertional
Added March 6, 2014
0 Communities
1 Members
0 Resources
9 MeSH Terms
Conditional gene-trap mutagenesis in zebrafish.
Maddison LA, Li M, Chen W
(2014) Methods Mol Biol 1101: 393-411
MeSH Terms: Alleles, Animals, Base Sequence, DNA Transposable Elements, Embryo Culture Techniques, Female, Genetic Vectors, Male, Microinjections, Mutagenesis, Insertional, Organ Specificity, Phenotype, Zebrafish
Added April 24, 2014
0 Communities
1 Members
0 Resources
13 MeSH Terms
Strong cross-system interactions drive the activation of the QseB response regulator in the absence of its cognate sensor.
Guckes KR, Kostakioti M, Breland EJ, Gu AP, Shaffer CL, Martinez CR, Hultgren SJ, Hadjifrangiskou M
(2013) Proc Natl Acad Sci U S A 110: 16592-7
MeSH Terms: Bacterial Proteins, DNA Primers, DNA Transposable Elements, Electrophoretic Mobility Shift Assay, Escherichia coli, Escherichia coli Proteins, Gene Expression Regulation, Bacterial, Immunoblotting, Mutagenesis, Quorum Sensing, Real-Time Polymerase Chain Reaction, Receptor Cross-Talk, Signal Transduction
Added August 6, 2014
0 Communities
1 Members
0 Resources
13 MeSH Terms