Other search tools

About this data

The publication data currently available has been vetted by Vanderbilt faculty, staff, administrators and trainees. The data itself is retrieved directly from NCBI's PubMed and is automatically updated on a weekly basis to ensure accuracy and completeness.

If you have any questions or comments, please contact us.

Results: 181 to 184 of 184

Publication Record

Connections

The endo-8,9-epoxide of aflatoxin B1: a new metabolite.
Raney KD, Coles B, Guengerich FP, Harris TM
(1992) Chem Res Toxicol 5: 333-5
MeSH Terms: Aflatoxin B1, Animals, Crystallization, Humans, In Vitro Techniques, Magnetic Resonance Spectroscopy, Microsomes, Liver, Rats, Stereoisomerism
Added March 5, 2014
0 Communities
1 Members
0 Resources
9 MeSH Terms
Glutathione conjugation of aflatoxin B1 exo- and endo-epoxides by rat and human glutathione S-transferases.
Raney KD, Meyer DJ, Ketterer B, Harris TM, Guengerich FP
(1992) Chem Res Toxicol 5: 470-8
MeSH Terms: Aflatoxin B1, Animals, Catalysis, Cytosol, Glutathione, Glutathione Transferase, Humans, In Vitro Techniques, Liver, Magnetic Resonance Spectroscopy, Male, Mice, Rats, Rats, Sprague-Dawley, Stereoisomerism, Sulfhydryl Compounds
Show Abstract · Added March 5, 2014
Much evidence supports the view that the rate of conjugation of glutathione (GSH) with aflatoxin B1 (AFB1) exo-epoxide is an important factor in determining the species variation in risk to aflatoxins and that induction of GSH S-transferases can yield a significant protective effect. An assay has been developed in which the enzymatic formation of the conjugates of GSH and AFB1 exo-epoxide and the recently described AFB1 endo-epoxide is measured directly. 1H NMR spectra are reported for both the AFB1 exo- and endo-epoxide-GSH conjugates. Structural assignments were made by comparison with AFB1 exo- and endo-epoxide-ethanethiol conjugates, for which nuclear Overhauser effects were measured to establish relative configurations. The endo-epoxide was found to be a good substrate for GSH conjugate formation in rat liver cytosol while mouse liver cytosol conjugated the exo-epoxide almost exclusively. Human liver cytosol conjugated both epoxide isomers to much lower extents than did cytosols prepared from rats or mice. Purified rat GSH S-transferases catalyzed the formation of the AFB1 exo-epoxide-GSH conjugate in the order 1-1 approximately 4-4 approximately 3-3 greater than 2-2 greater than 4-6 (7-7 and 8-8 did not form the exo-epoxide-GSH conjugate at levels above the nonenzymatic rate). The only rat GSH S-transferases that conjugated the endo-epoxide were 4-4 and 4-6, with 4-4 being the more active.(ABSTRACT TRUNCATED AT 250 WORDS)
0 Communities
1 Members
0 Resources
16 MeSH Terms
Quantitative analysis of methadone in biological fluids using deuterium-labeled methadone and GLC-chemical-ionization mass spectrometry.
Hachey DL, Kreek MJ, Mattson DH
(1977) J Pharm Sci 66: 1579-82
MeSH Terms: Adult, Chromatography, Gas, Deuterium, Humans, Isotope Labeling, Mass Spectrometry, Methadone, Methods, Stereoisomerism
Show Abstract · Added March 20, 2014
The (+)-,(-)-, and (+/-)-2H5-methadones, which contained five deuterium atoms in one aromatic ring, were synthesized for use in clinical pharmacological studies and as internal standards. GLC-chemical-ionization mass spectrometry was used to determine plasma and urinary methadone levels by an inverse isotope dilution assay. Plasma drug levels could be determined to 10 pmoles/ml, and urine levels could be measured to 5 pmoles/ml. Plasma methadone levels were examined in several patients undergoing methadone maintenance therapy. These levels generally ranged between 100 and 400 ng/ml (320-1300 pmoles/ml) after an average oral dose of 1 mg/kg/day. The methadone half-life was 28.8 +/- 4.8 hr.
0 Communities
1 Members
0 Resources
9 MeSH Terms
Stereoselective disposition of methadone in man.
Kreek MJ, Hachey DL, Klein PD
(1979) Life Sci 24: 925-32
MeSH Terms: Adult, Female, Half-Life, Humans, Kinetics, Male, Methadone, Stereoisomerism
Added March 20, 2014
0 Communities
1 Members
0 Resources
8 MeSH Terms