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Results: 1871 to 1876 of 1876

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The effect of somatostatin on glucose uptake and production by rat tissues in vitro.
Cherrington AD, Caldwell MD, Dietz MR, Exton JH, Crofford OB
(1977) Diabetes 26: 740-8
MeSH Terms: Adipose Tissue, Animals, Cyclic AMP, Dose-Response Relationship, Drug, Glucagon, Glucose, Hindlimb, In Vitro Techniques, Insulin, Liver, Male, Muscles, Protein Kinases, Rats, Somatostatin
Added December 10, 2013
0 Communities
1 Members
0 Resources
15 MeSH Terms
Studies on the role of cAMP-dependent protein kinase in the actions of glucagon and catecholamines on liver glycogen metabolism.
Cherrington AD, Exton JH
(1976) Metabolism 25: 1351-4
MeSH Terms: Animals, Cyclic AMP, Dose-Response Relationship, Drug, Epinephrine, Glucagon, Liver Glycogen, Phenylephrine, Phosphorylases, Protein Kinases, Rats, Receptors, Adrenergic
Show Abstract · Added December 10, 2013
Glucagon causes a rapid activation of cAMP-dependent protein kinase in rat liver parenchymal cells which correlates well with the accumulation of cAMP. Full activation of phosphorylase or inactivation of glycogen synthase is achieved with half-maximal or less activation of protein kinase. Epinephrine stimulates glycogen breakdown in these cells mainly by mechanisms involving alpha-adrenergic receptors and not beta-receptors. Activition of alpha-receptors results in rapid activation of phosphorylase and inactivation of glycogen synthase without accumulation of cAMP or activation of cAMP-dependent protein kinase. Activation of beta-receptors causes a transient rise in cAMP and a short-lived activation of protein kinase with correspondingly little stimulation of glycogenolysis.
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1 Members
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11 MeSH Terms
Metabolism of diethylstilbestrol: identification of a catechol derived from dienestrol.
Weidenfeld J, Carter P, Reinhold VN, Tanner SB, Engel LL
(1978) Biomed Mass Spectrom 5: 587-90
MeSH Terms: Animals, Basidiomycota, Catechols, Dienestrol, Diethylstilbestrol, Female, Mass Spectrometry, Microsomes, Liver, Monophenol Monooxygenase, Oxidation-Reduction, Phenazines, Phenols, Rats
Show Abstract · Added January 20, 2015
The enzymatic oxidation of E-3,4-bis-(p-hydroxyphenyl)-hex-3-ene (diethylstilbestrol) by either mushroom tyrosinase or rat liver microsomes in the presence of NADPH and air yields a catechol. Upon further oxidation of both compounds with periodate and condensation of the resulting o-quinones with o-phenylenediamine, phenazines are produced. The phenazines derived from the products of both the plant and animal enzyme systems are identical to the product obtained by oxidation of diethylstilbestrol with potassium nitrosodisulfonate and condensation of the o-quinone produced with o-phenylenediamine. High and low resolution mass spectra of the phenazine are consistent with its derivation from a catechol having two fewer hydrogens than diethylstilbestrol.
0 Communities
1 Members
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13 MeSH Terms
Comparative studies on highly metabolically active histone acetylation.
Moore M, Jackson V, Sealy L, Chalkley R
(1979) Biochim Biophys Acta 561: 248-60
MeSH Terms: Acetylation, Animals, Cell Cycle, Cell Division, Cell Line, Dactinomycin, Histone Deacetylases, Histones, Liver Neoplasms, Experimental, Mitosis, Neoplasm Proteins, Skin, Tetrahymena pyriformis, Thymus Gland
Show Abstract · Added March 5, 2014
Histone acetate is hydrolyzed rapidly in logarithmically dividing hepatoma tissue culture cells (Jackson, V., Shires, A., Chalkley, R. and Granner, D.K. (1975) J. Biol. Chem. 250, 4856--4863). The phenomenon has been analyzed further in hepatoma tissue culture cells at various stages of the cell cycle, in stationary phase, and in the presence of actinomycin D. We also investigated the phenomenon in Tetrahymena pyriformis macronuclei, bovine thymocytes, and human foreskin fibroblasts. The data suggest that this highly metabolically active histone acetylation while altered in mitotic cells, is independent of the overall rate of cell division, and is only slightly sensitive to actinomycin D. Finally, we conclude that the same general phenomenon is found in both cancerous and normal cells and is apparently common to cells from various stages of the evolutionary scale.
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14 MeSH Terms
Studies on the alpha-andrenergic activation of hepatic glucose output. II. Investigation of the roles of adenosine 3':5'-monophosphate and adenosine 3':5'-monophosphate-dependent protein kinase in the actions of phenylephrine in isolated hepatocytes.
Cherrington AD, Assimacopoulos FD, Harper SC, Corbin JD, Park CR, Exton JH
(1976) J Biol Chem 251: 5209-18
MeSH Terms: Adrenergic alpha-Agonists, Animals, Cyclic AMP, Enzyme Activation, Fluorides, Glucagon, Glucose, Liver, Magnesium, Osmolar Concentration, Phenoxybenzamine, Phenylephrine, Potassium Chloride, Propranolol, Protein Kinases, Rats, Xanthines
Show Abstract · Added December 10, 2013
The effects of the alpha-adrenergic agonist phenylephrine on the levels of adenosine 3':5'-monophosphate (cAMP) and the activity of the cAMP-dependent protein kinase in isolated rat liver parenchymal cells were studied. Cyclic AMP was very slightly (5 to 13%) increased in cells incubated with phenylephrine at a concentration (10(-5) M) which was maximally effective on glycogenolysis and gluconeogenesis. However, the increase was significant only at 5 min. Cyclic AMP levels with 10(-5) M phenylephrine measured at this time were reduced by the beta-adrenergic antagonist propranolol, but were unaffected by the alpha-blocker phenoxybenzamine, indicating that the elevation was due to weak beta activity of the agonist. When doses of glucagon, epinephrine, and phenylephrine which produced the same stimulation of glycogenolysis or gluconeogenesis were added to the same batches of cells, there were marked rises in cAMP with glucagon, minimal increases with epinephrine, and little or no changes with phenylephrine, indicating that the two catecholamine stimulated these processes largely by mechanisms not involving cAMP accumulation. DEAE-cellulose chromatography of homogenates of liver cells revealed two major peaks of cAMP-dependent protein kinase activity. These eluted at similar salt concentrations as the type I and II isozymes from rat heart. Optimal conditions for preservation of hormone effects on the activity of the enzyme in the cells were determined. High concentrations of phenylephrine (10(-5) M and 10(-4) M) produced a small increase (10 tp 16%) in the activity ratio (-cAMP/+cAMP) of the enzyme. This was abolished by propranolol, but not by phenoxybenzamine, indicating that it was due to weak beta activity of the agonist. The increase in the activity ratio of the kinase with 10(-5) M phenylephrine was much smaller than that produced by a glycogenolytically equivalent dose of glucagon. The changes in protein kinase induced by phenylephrine and the blockers and by glucagon were thus consistent with those in cAMP. Theophylline and 1-methyl-3-isobutylxanthine, which inhibit cAMP phosphodiesterase, potentiated the effects of phenylephrine on glycogenolysis and gluconeogenesis. The potentiations were blocked by phenoxybenzamine, but not by propranolol. Methylisobutylxanthine increased the levels of cAMP and enhanced the activation of protein kinase in cells incubated with phenylephrine. These effects were diminished or abolished by propanolol, but were unaffected by phenoxybenzamine. It is concluded from these data that alpha-adrenergic activation of glycogenolysis and gluconeogenesis in isolated rat liver parenchymal cells occurs by mechanisms not involving an increase in total cellular cAMP or activation of the cAMP-dependent protein kinase. The results also show that phosphodiesterase inhibitors potentiate alpha-adrenergic actions in hepatocytes mainly by a mechanism(s) not involving a rise in cAMP.
0 Communities
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17 MeSH Terms
Digitoxin metabolism by rat liver microsomes.
Schmoldt A, Benthe HF, Haberland G
(1975) Biochem Pharmacol 24: 1639-41
MeSH Terms: Animals, Chromatography, Thin Layer, Digitoxigenin, Digitoxin, Hydroxylation, In Vitro Techniques, Male, Microsomes, Liver, NADP, Rats, Time Factors
Added February 9, 2015
1 Communities
0 Members
0 Resources
11 MeSH Terms