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Results: 15041 to 15050 of 15075

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Role of protein and RNA synthesis in the development of insulin binding sites on activated thymus-derived lymphocytes.
Helderman JH, Strom TB
(1979) J Biol Chem 254: 7203-7
MeSH Terms: Amanitins, Animals, Cells, Cultured, Cycloheximide, DNA, DNA Replication, Dactinomycin, Lectins, Lymphocyte Activation, Male, Protein Biosynthesis, RNA, Rats, Receptor, Insulin, T-Lymphocytes, Transcription, Genetic
Added April 21, 2014
0 Communities
1 Members
0 Resources
16 MeSH Terms
Emergence of insulin receptors upon alloimmune T cells in the rat.
Helderman JH, Strom TB
(1977) J Clin Invest 59: 338-44
MeSH Terms: Animals, Cell Membrane, Cytotoxicity Tests, Immunologic, Lymphocyte Depletion, Rats, Receptor, Insulin, T-Lymphocytes
Show Abstract · Added April 21, 2014
Insulin, as well as other ligands which increase intracellular guanosine 3',5'-cyclic monophosphate (cGMP), augments thymic-derived (T)- lymphocyte effector activity as revealed by alloimmune lymphocyte-mediated cytotoxicity. The observation that insulin binds only to monocytes among circulating nonimmune human mononuclear cells fosterd reexamination of the mechanism by which insulin augments T-lymphocyte function. This report concerns a test of the hypothesis that the T cell is directly affected by insulin and that an insulin receptor emerges upon T lymphocytes consequent to immune activation. Spleens were removed from rats skin grafted across a major histocompatibility barrier. Lymphocytes were harvested from Ficoll-Hypaque density gradients and subsequently enriched for T cells by passage over one or two nylon wool columns. This population was composed of more than 98% T cells as assessed by surface marker techniques (Ig staining, erythrocyte antibody, and erythrocyte antibody complement rosetting, anti-T staining). There was no loss of augmentation of lymphocyte-mediated cytotoxicity induced by insulin, carbamycholine, and 8-bromo-cGMP in the purified cells when compared to unfractionated cells 7 days after transplantation. 125I-insulin bound saturably to the allostimulated T-enriched lymphocytes with maximum binding at 12.8 +/- 0.2 pg and a dissociation constant at equilibrium of 1.3 nM. In contrast, insulin receptors were not present on nonimmune T-enriched cells or on T cells from animals that received syngeneic grafts. The affinity of the lymphocyte insulin receptor was similar to that of more conventional insulin-sensitive tissues e.g., liver, adipocyte. After 89% of T cells from spleens on day 7 were lysed with anti-thy 1.1 antibody and complement, the ability to measure specific insulin binding was lost. These data confirm a physiologic role for insulin in T-lymphocyte effector function and describe the emergence of insulin receptors concomitant with cell sensitivity to ligand. Such receptors may play a role in hormonal modulation of the immune response.
0 Communities
1 Members
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7 MeSH Terms
Fibronectin and the multiple interaction model for platelet-collagen adhesion.
Santoro SA, Cunningham LW
(1979) Proc Natl Acad Sci U S A 76: 2644-8
MeSH Terms: Animals, Blood Platelets, Blood Proteins, Cell Adhesion, Chymotrypsin, Collagen, Immunoglobulin Fab Fragments, Kinetics, Membrane Proteins, Platelet Aggregation, Rats
Show Abstract · Added March 5, 2014
A rapid, sensitive, and reproducible assay to determine the adhesion of platelets to collagen has been developed. Collagen fibers and adherent platelets are retained on polycarbonate membrane filters. Chemical modification of collagen by acetylation and of platelets by treatment with chymotrypsin markedly reduces adhesion. The role of fibronectin in the collagen-platelet interaction has been examined. Treatment of platelets with purified antibody or Fab' fragments to fibronectin only slightly reduces adhesion. Preincubation of platelets with high concentrations of gelatin reduces adhesion by only 22% but fails to inhibit aggregation. Thus, fibronectin has only a limited role in the adhesion of platelets to collagen and is either not involved in the adhesion that leads to aggregation or is only one of several adhesion mechanisms, any of which alone can initiate aggregation.
1 Communities
1 Members
0 Resources
11 MeSH Terms
[Replacement of saccharose with other sweetening agents and their effect on experimental caries in rats].
KurhaĊ„ska A, Buchowski M, Gawecki J
(1979) Czas Stomatol 32: 143-8
MeSH Terms: Animals, Cariogenic Agents, Dental Caries, Glucose, Glycine, Rats, Sorbitol, Sucrose, Sweetening Agents
Added July 31, 2015
0 Communities
1 Members
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9 MeSH Terms
A radioimmunometric antibody-binding assay for evaluation of xenoantisera to melanoma-associated antigens.
McCabe RP, Quaranta V, Frugis L, Ferrone S, Reisfeld RA
(1979) J Natl Cancer Inst 62: 455-63
MeSH Terms: Animals, Antibodies, Neoplasm, Antibody Specificity, Antigens, Neoplasm, Cell Line, Cytotoxicity Tests, Immunologic, Humans, Immunoglobulin G, Male, Melanoma, Neoplasms, Experimental, Rabbits, Radioimmunoassay, Staphylococcal Protein A
Show Abstract · Added March 27, 2014
A radioimmunometric antibody-binding assay was developed with the use of 125I-labeled protein A of Staphylococcus aureus (SpA) for the evaluation of xenoantisera to human melanoma-associated antigens. Antisera were produced in New Zealand male albino rabbits by the injection of cultured human melanoma cells or soluble, partially purified melanoma-associated antigens isolated from these cells. Xenoantisera were rendered operationally specific for melanoma-associated antigens by absorption with human red cells and cultured lymphoblasts. The methodologic parameters and the quantitative relationships among xenoantisera, cultured melanoma target cells, and 125I-labeled SpA and their effect on the measurement of xenoantibody binding were critically evaluated. Data indicated the usefulness of the radioimmunometric assay in monitoring the efficacy of absorption and in characterizing the specificity of xenoantisera to melanoma-associated antigens. The radioimmunometric binding assay when modified and used as a binding inhibition assay was effective in the assessment of the serologic activity of soluble melanoma-associated antigens and thus may be used to monitor the progress of antigen purification.
1 Communities
1 Members
0 Resources
14 MeSH Terms
Mutual entrainment of bilaterally distributed circadian pacemaker.
Page TL, Caldarola PC, Pittendrigh CS
(1977) Proc Natl Acad Sci U S A 74: 1277-81
MeSH Terms: Animals, Biological Clocks, Circadian Rhythm, Cockroaches, Functional Laterality, Light, Motor Activity, Ocular Physiological Phenomena, Optic Lobe, Nonmammalian
Show Abstract · Added May 29, 2014
The interactions between the bilaterally distributed components of the circadin system that controls the locomotor activity rhythm of the cockroach Leucophaea maderae were investigated in a series of surgical lesion experiments. Complete excision of one optic lobe (either right or left) or its surgical isolation from the central nervous system had no effect on the animals' ability to free-run in constant darkness nor was there any indication, as judged by postoperative pi values of any difference between left and right lobe pacemakers. However, these surgical procedures consistently resulted in a significant increase in tau over preoperative value while optic nerve section had no effect on tau. The propostion is developed that the left and right pacemakers in the two optic lobes are mutally coupled and that the compound pacemaker's period is shorter than either of its constituent pacemakers. It was also found that the integrity of either compound eye is sufficient to assure entrainment of both left and right pacemakers.
0 Communities
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9 MeSH Terms
Primary hemostasis (collagen-platelet interaction): introduction.
Cunningham LW, Santoro S, Davies H
(1978) Suppl Thromb Haemost 63: 205-13
MeSH Terms: Animals, Blood Platelets, Calcium, Collagen, Hemostasis, Humans, Platelet Aggregation, Rats, Skin Physiological Phenomena, Wound Healing
Added March 5, 2014
1 Communities
1 Members
0 Resources
10 MeSH Terms
Analysis of the cold lability behavior of rabbit muscle phosphofructokinase.
Bock PE, Gilbert HR, Frieden C
(1975) Biochem Biophys Res Commun 66: 564-9
MeSH Terms: Animals, Cold Temperature, Drug Stability, Hydrogen-Ion Concentration, Kinetics, Muscles, Phosphofructokinase-1, Protein Denaturation, Rabbits
Added January 20, 2015
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1 Members
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9 MeSH Terms
The effects of ACTH on lung maturation in fetal lambs.
Sundell HW, Gray ME, Relier JP, Kovar IZ, Catterton WZ, Swift LL, Stahlman MT
(1979) Am J Pathol 97: 393-410
MeSH Terms: Adrenocorticotropic Hormone, Animals, Animals, Newborn, Female, Gestational Age, Humans, Hyaline Membrane Disease, Infant, Newborn, Infusions, Parenteral, Lipids, Lung, Organ Size, Pregnancy, Sheep
Show Abstract · Added May 27, 2014
The ability of adrenocorticotrophic hormone (ACTH) to induce lung maturation was evaluated in fetal lambs. One member of 14 twin pairs between 114 and 138 days of gestation was infused intravenously with 0.5 mg ACTH over 5 days. The lungs of treated versus control lambs were judged more mature by morphologic criteria by the use of light and electron microscopy and by biochemical criteria by the use of lamellar-body-rich cell fractions. None of 5 premature lambs treated with ACTH and allowed to breathe showed evidence of hyaline membrane disease, while 3 untreated control lambs showed typical hyaline membranes.
0 Communities
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14 MeSH Terms
Structural characteristics of nitrosyl hemoglobins and their relation to ESR spectra.
John ME, Waterman MR
(1979) FEBS Lett 106: 219-22
MeSH Terms: Animals, Binding Sites, Electron Spin Resonance Spectroscopy, Hemoglobins, Hemoglobins, Abnormal, Humans, Nitric Oxide, Opossums, Protein Binding, Protein Conformation, Rabbits, Rats
Added February 12, 2015
0 Communities
1 Members
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12 MeSH Terms