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Results: 111 to 112 of 112

Publication Record


"Designer membranes": construction of a cell containing multiple membrane-bound cytochromes P450.
Estabrook RW, Trant JM, Mathew PA, Mason JI, Waterman MR
(1992) Curr Top Cell Regul 33: 419-31
MeSH Terms: Animals, Cattle, Cell Line, Transformed, Chlorocebus aethiops, Cytochrome P-450 Enzyme System, Humans, Intracellular Membranes, Isoenzymes, Kidney, Membrane Proteins, Microsomes, Liver, Multienzyme Complexes, Plasmids, Protein Engineering, Rats, Recombinant Proteins, Saccharomyces cerevisiae, Steroids, Transfection
Added February 12, 2015
0 Communities
1 Members
0 Resources
19 MeSH Terms
Differential regulation of transforming growth factor alpha autoinduction in a nontransformed and transformed epithelial cell.
Coffey RJ, Graves-Deal R, Dempsey PJ, Whitehead RH, Pittelkow MR
(1992) Cell Growth Differ 3: 347-54
MeSH Terms: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine, Cell Line, Transformed, Colon, Gene Expression Regulation, Neoplastic, Humans, Isoquinolines, Keratinocytes, Piperazines, Protein Kinase C, RNA, Messenger, Signal Transduction, Transcription, Genetic, Transforming Growth Factor alpha
Show Abstract · Added March 27, 2014
Addition of transforming growth factor alpha (TGF-alpha) to cultured human keratinocytes results in enhanced expression of TGF-alpha mRNA. This phenomenon of TGF-alpha autoinduction is also observed in a TGF-alpha responsive colon cancer cell line, LIM 1215. In the present study, regulation of TGF-alpha autoinduction is examined in these two cell types. In human keratinocytes, but not in LIM 1215 cells, the increase in steady-state TGF-alpha mRNA following administration of TGF-alpha is due to stabilization of the 4.8-kilobase TGF-alpha transcript, as determined by actinomycin D decay curves. Nuclear run-on experiments confirmed transcriptional control in LIM 1215 cells. Basal and TGF-alpha-stimulated TGF-alpha expression is mediated, at least in part, through a protein kinase C-dependent pathway in both cell types, as determined by the protein kinase C inhibitor 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H7), which attenuates TGF-alpha mRNA accumulation. In the keratinocytes, but not in the LIM 1215 cells, basal TGF-alpha expression is mediated through an epidermal growth factor receptor-dependent pathway, as determined by antibody blockade of the epidermal growth factor receptor. Thus, differential regulation of TGF-alpha autoinduction exists in these nontransformed and transformed epithelial cell types.
1 Communities
1 Members
0 Resources
13 MeSH Terms