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Results: 1 to 5 of 5

Publication Record


Yersinia enterocolitica exploits different pathways to accomplish adhesion and toxin injection into host cells.
Keller B, Mühlenkamp M, Deuschle E, Siegfried A, Mössner S, Schade J, Griesinger T, Katava N, Braunsdorf C, Fehrenbacher B, Jiménez-Soto LF, Schaller M, Haas R, Genth H, Retta SF, Meyer H, Böttcher RT, Zent R, Schütz M, Autenrieth IB, Bohn E
(2015) Cell Microbiol 17: 1179-204
MeSH Terms: Adhesins, Bacterial, Bacterial Adhesion, Bacterial Toxins, Epithelial Cells, Fibroblasts, Flow Cytometry, Host-Pathogen Interactions, Integrin alphaV, Integrin beta1, Microscopy, Electron, Protein Binding, Protein Transport, Yersinia enterocolitica
Show Abstract · Added February 4, 2016
The current paradigm suggests that Yersinia enterocolitica (Ye) adheres to host cells via the outer membrane proteins Yersinia adhesin A (YadA) or invasin (Inv) to facilitate injection of Yops by the type III secretion system. In this process Inv binds directly to β1 integrins of host cells while YadA may bind indirectly via extracellular matrix proteins to β1 integrins. Here we challenged this paradigm and investigated the requirements for Yop injection. We demonstrate that Inv- but not YadA-mediated adhesion depends on β1 integrin binding and activation, and that tight adhesion is a prerequisite for Yop injection. By means of novel transgenic cell lines, shRNA approaches and RGD peptides, we found that YadA, in contrast to Inv, may use a broad host cell receptor repertoire for host cell adhesion. In the absence of β1 integrins, YadA mediates Yop injection by interaction with αV integrins in cooperation with yet unknown cofactors expressed by epithelial cells, but not fibroblasts. Electron microscopic and flow chamber studies revealed that a defined intimate contact area between Ye and host cells resulting in adhesion forces resisting shear stress is required for Yop injection. Thus, the indirect binding of YadA to a broad extracellular matrix (ECM) binding host cell receptor repertoire of different cell types makes YadA a versatile tool to ensure Yop injection. In conclusion, given the differential expression of the outer membrane proteins Inv and YadA in the course of Ye infection and differential expression of integrins by various host cell populations, the data demonstrate that Ye is flexibly armed to accomplish Yop injection in different host cell types, a central event in its immune evasion strategy.
© 2015 John Wiley & Sons Ltd.
1 Communities
1 Members
0 Resources
13 MeSH Terms
When nature meets nurture: persistent Yersinia infection.
Watson GT, Huaman MA, Semler MW, Manners J, Woron AM, Carpenter LR, Christman BW
(2013) Am J Med 126: 578-80
MeSH Terms: Drinking Water, Humans, Iron Overload, Liver Abscess, Male, Middle Aged, Yersinia Infections, Yersinia enterocolitica
Added February 19, 2015
0 Communities
1 Members
0 Resources
8 MeSH Terms
Protective roles for fibrin, tissue factor, plasminogen activator inhibitor-1, and thrombin activatable fibrinolysis inhibitor, but not factor XI, during defense against the gram-negative bacterium Yersinia enterocolitica.
Luo D, Szaba FM, Kummer LW, Plow EF, Mackman N, Gailani D, Smiley ST
(2011) J Immunol 187: 1866-76
MeSH Terms: Animals, Carboxypeptidase B2, Factor XI, Fibrin, Humans, Liver, Mice, Mice, Knockout, Sepsis, Serpin E2, Thromboplastin, Yersinia Infections, Yersinia enterocolitica
Show Abstract · Added May 19, 2014
Septic infections dysregulate hemostatic pathways, prompting coagulopathy. Nevertheless, anticoagulant therapies typically fail to protect humans from septic pathology. The data reported in this work may help to explain this discrepancy by demonstrating critical protective roles for coagulation leading to fibrin deposition during host defense against the Gram-negative bacterium Yersinia enterocolitica. After i.p. inoculation with Y. enterocolitica, fibrinogen-deficient mice display impaired cytokine and chemokine production in the peritoneal cavity and suppressed neutrophil recruitment. Moreover, both gene-targeted fibrinogen-deficient mice and wild-type mice treated with the anticoagulant coumadin display increased hepatic bacterial burden and mortality following either i.p. or i.v. inoculation with Y. enterocolitica. Mice with low tissue factor activity succumb to yersiniosis with a phenotype similar to fibrin(ogen)-deficient mice, whereas factor XI-deficient mice show wild-type levels of resistance. Mice deficient in plasminogen activator inhibitor-1 or thrombin-activatable fibrinolysis inhibitor display modest phenotypes, but mice deficient in both plasminogen activator inhibitor-1 and thrombin-activatable fibrinolysis inhibitor succumb to yersiniosis with a phenotype resembling fibrin(ogen)-deficient mice. These findings demonstrate critical protective roles for the tissue factor-dependent extrinsic coagulation pathway during host defense against bacteria and caution that therapeutics targeting major thrombin-generating or antifibrinolytic pathways may disrupt fibrin-mediated host defense during Gram-negative sepsis.
0 Communities
1 Members
0 Resources
13 MeSH Terms
Detection of Escherichia coli, Salmonella spp., Shigella spp., Yersinia enterocolitica, Vibrio cholerae, and Campylobacter spp. enteropathogens by 3-reaction multiplex polymerase chain reaction.
Gómez-Duarte OG, Bai J, Newell E
(2009) Diagn Microbiol Infect Dis 63: 1-9
MeSH Terms: Campylobacter, Cloning, Molecular, DNA Primers, Developing Countries, Diarrhea, Electrophoresis, Agar Gel, Enterobacteriaceae, Enteropathogenic Escherichia coli, Genes, Bacterial, Humans, Polymerase Chain Reaction, Reproducibility of Results, Salmonella, Sensitivity and Specificity, Shigella, Vibrio cholerae, Yersinia enterocolitica
Show Abstract · Added May 27, 2014
The magnitude of bacterial diarrhea in developing countries is largely unknown because affordable detection methods are not available. We have developed a polymerase chain reaction (PCR)-based assay for use in areas with limited resources to screen for diarrheogenic strains from clinical isolates. To simplify the assay and minimize reagents, our method implemented the use of plasmids rather than bacteria as template controls and the use of bacterial suspensions or crude DNA preparations rather than purified genomic DNA as template DNA. The assay consisted of 3 PCR reactions using 3 groups of 5 to 6 primer pairs to identify the 11 most common bacterial diarrheogenic pathogens. The 3-reaction multiplex PCR amplifies DNA targets specific for each 1 of the 6 Escherichia coli diarrheogenic strains and the 5 non-E. coli diarrheogenic strains, including Salmonella spp., Shigella spp., Campylobacter spp., Yersinia enterocolitica, and Vibrio cholerae. The assay may provide an important epidemiologic tool to investigate the role of diarrheogenic bacterial pathogens in areas of the world with limited resources.
0 Communities
1 Members
0 Resources
17 MeSH Terms
Yersinia enterocolitica.
Cover TL, Aber RC
(1989) N Engl J Med 321: 16-24
MeSH Terms: Humans, Yersinia Infections, Yersinia enterocolitica
Added March 5, 2014
0 Communities
1 Members
0 Resources
3 MeSH Terms