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Results: 1 to 10 of 109

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A recommended and verified procedure for in situ tryptic digestion of formalin-fixed paraffin-embedded tissues for analysis by matrix-assisted laser desorption/ionization imaging mass spectrometry.
Judd AM, Gutierrez DB, Moore JL, Patterson NH, Yang J, Romer CE, Norris JL, Caprioli RM
(2019) J Mass Spectrom 54: 716-727
MeSH Terms: Formaldehyde, Humans, Paraffin Embedding, Proteins, Proteolysis, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tissue Array Analysis, Tissue Fixation, Trypsin
Added October 15, 2019
0 Communities
2 Members
0 Resources
MeSH Terms
MicroLESA: Integrating Autofluorescence Microscopy, In Situ Micro-Digestions, and Liquid Extraction Surface Analysis for High Spatial Resolution Targeted Proteomic Studies.
Ryan DJ, Patterson NH, Putnam NE, Wilde AD, Weiss A, Perry WJ, Cassat JE, Skaar EP, Caprioli RM, Spraggins JM
(2019) Anal Chem 91: 7578-7585
MeSH Terms: Animals, Fluorescent Dyes, Kidney, Liquid-Liquid Extraction, Mice, Microscopy, Fluorescence, Peptides, Proteins, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staphylococcus aureus, Trypsin
Added January 22, 2020
0 Communities
3 Members
0 Resources
12 MeSH Terms
Synthesis and Characterization of Site-Specific O -Alkylguanine DNA-Alkyl Transferase-Oligonucleotide Crosslinks.
Ghodke PP, Albertolle ME, Johnson KM, Guengerich FP
(2019) Curr Protoc Nucleic Acid Chem 76: e74
MeSH Terms: Catalysis, Catalytic Domain, Chromatography, Liquid, Copper, Cross-Linking Reagents, Escherichia coli, O(6)-Methylguanine-DNA Methyltransferase, Oligonucleotides, Polymerization, Tandem Mass Spectrometry, Templates, Genetic, Trypsin
Added March 3, 2020
0 Communities
1 Members
0 Resources
MeSH Terms
Enhanced Spatially Resolved Proteomics Using On-Tissue Hydrogel-Mediated Protein Digestion.
Rizzo DG, Prentice BM, Moore JL, Norris JL, Caprioli RM
(2017) Anal Chem 89: 2948-2955
MeSH Terms: Acrylic Resins, Animals, Cerebellum, Chromatography, High Pressure Liquid, Hydrogels, Liver, Proteins, Proteolysis, Rats, Tandem Mass Spectrometry, Trypsin
Added March 10, 2017
1 Communities
2 Members
0 Resources
11 MeSH Terms
Trypsin and MALDI matrix pre-coated targets simplify sample preparation for mapping proteomic distributions within biological tissues by imaging mass spectrometry.
Zubair F, Laibinis PE, Swisher WG, Yang J, Spraggins JM, Norris JL, Caprioli RM
(2016) J Mass Spectrom 51: 1168-1179
MeSH Terms: Animals, Brain Chemistry, Molecular Imaging, Peptide Fragments, Peptide Mapping, Proteomics, Rats, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Trypsin
Added March 10, 2017
1 Communities
3 Members
0 Resources
9 MeSH Terms
Histology-guided protein digestion/extraction from formalin-fixed and paraffin-embedded pressure ulcer biopsies.
Taverna D, Pollins AC, Nanney LB, Sindona G, Caprioli RM
(2016) Exp Dermatol 25: 143-6
MeSH Terms: Biopsy, Chromatography, Liquid, Eosine Yellowish-(YS), Formaldehyde, Hematoxylin, Humans, Hydrogels, Paraffin Embedding, Pressure Ulcer, Proteins, Proteomics, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling, Tandem Mass Spectrometry, Tissue Fixation, Trypsin
Added October 15, 2015
1 Communities
1 Members
0 Resources
17 MeSH Terms
An Efficient Approach to Evaluate Reporter Ion Behavior from MALDI-MS/MS Data for Quantification Studies Using Isobaric Tags.
Cologna SM, Crutchfield CA, Searle BC, Blank PS, Toth CL, Ely AM, Picache JA, Backlund PS, Wassif CA, Porter FD, Yergey AL
(2015) J Proteome Res 14: 4169-78
MeSH Terms: Complex Mixtures, Hep G2 Cells, Humans, Ions, Peptides, Proteolysis, Proteome, Proteomics, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling, Tandem Mass Spectrometry, Trypsin
Added April 25, 2016
0 Communities
1 Members
0 Resources
12 MeSH Terms
Rationale and Design of the Genomic Research in Alpha-1 Antitrypsin Deficiency and Sarcoidosis (GRADS) Study. Sarcoidosis Protocol.
Moller DR, Koth LL, Maier LA, Morris A, Drake W, Rossman M, Leader JK, Collman RG, Hamzeh N, Sweiss NJ, Zhang Y, O'Neal S, Senior RM, Becich M, Hochheiser HS, Kaminski N, Wisniewski SR, Gibson KF, GRADS Sarcoidosis Study Group
(2015) Ann Am Thorac Soc 12: 1561-71
MeSH Terms: Adolescent, Adult, Aged, Aged, 80 and over, Biomarkers, Bronchoalveolar Lavage, Bronchoscopy, Cohort Studies, Female, Genomics, Humans, Lung, Male, Microbiota, Middle Aged, Research Design, Respiratory Function Tests, Sarcoidosis, Self Report, Tomography, X-Ray Computed, Young Adult, alpha 1-Antitrypsin Deficiency
Added February 4, 2016
0 Communities
1 Members
0 Resources
22 MeSH Terms
Histology-directed microwave assisted enzymatic protein digestion for MALDI MS analysis of mammalian tissue.
Taverna D, Norris JL, Caprioli RM
(2015) Anal Chem 87: 670-6
MeSH Terms: Animals, Brain, Chromatography, Liquid, Hydrogel, Polyethylene Glycol Dimethacrylate, Immunohistochemistry, Microwaves, Peptide Fragments, Proteolysis, Proteome, Proteomics, Rats, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tandem Mass Spectrometry, Trypsin
Added February 12, 2015
1 Communities
2 Members
0 Resources
14 MeSH Terms
Recombinant Drosophila prophenoloxidase 1 is sequentially cleaved by α-chymotrypsin during in vitro activation.
Lu A, Li X, Hillyer JF, Beerntsen BT, Söderhäll K, Ling E
(2014) Biochimie 102: 154-65
MeSH Terms: Animals, Catechol Oxidase, Chymotrypsin, Drosophila melanogaster, Enzyme Activation, Enzyme Precursors, Immunity, Innate, Recombinant Proteins
Added February 5, 2016
0 Communities
1 Members
0 Resources
8 MeSH Terms