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The publication data currently available has been vetted by Vanderbilt faculty, staff, administrators and trainees. The data itself is retrieved directly from NCBI's PubMed and is automatically updated on a weekly basis to ensure accuracy and completeness.

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Results: 1 to 10 of 13

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Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells.
Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH
(2017) Nucleic Acids Res 45: 8411-8422
MeSH Terms: Bacterial Proteins, CRISPR-Associated Protein 9, CRISPR-Cas Systems, Cell Line, Tumor, DNA Transposable Elements, Endonucleases, Gene Knockout Techniques, Gene Targeting, Gene Transfer Techniques, Humans, Hypoxanthine Phosphoribosyltransferase, Mutagenesis, Insertional, Recombinant Fusion Proteins, Reproducibility of Results, Transcription Activator-Like Effector Nucleases, Transcription Activator-Like Effectors, Transposases, Zinc Fingers
Added September 11, 2017
0 Communities
2 Members
0 Resources
18 MeSH Terms
Temporal self-regulation of transposition through host-independent transposase rodlet formation.
Woodard LE, Downes LM, Lee YC, Kaja A, Terefe ES, Wilson MH
(2017) Nucleic Acids Res 45: 353-366
MeSH Terms: Animals, DNA Transposable Elements, Female, Gene Expression Regulation, Genes, Reporter, HEK293 Cells, HeLa Cells, Humans, Insect Proteins, Luciferases, Male, Mice, Optical Imaging, Time-Lapse Imaging, Transposases, Tribolium
Added December 8, 2017
0 Communities
2 Members
0 Resources
16 MeSH Terms
Evaluating the potential for undesired genomic effects of the piggyBac transposon system in human cells.
Saha S, Woodard LE, Charron EM, Welch RC, Rooney CM, Wilson MH
(2015) Nucleic Acids Res 43: 1770-82
MeSH Terms: 5' Untranslated Regions, Chromosomes, Artificial, Bacterial, DNA Damage, DNA Transposable Elements, Genome, Human, Green Fluorescent Proteins, HEK293 Cells, Humans, Plasmids, Polymerase Chain Reaction, Transgenes, Transposases
Added January 30, 2015
0 Communities
2 Members
0 Resources
12 MeSH Terms
An adaptable system for improving transposon-based gene expression in vivo via transient transgene repression.
Doherty JE, Woodard LE, Bear AS, Foster AE, Wilson MH
(2013) FASEB J 27: 3753-62
MeSH Terms: Animals, DNA Transposable Elements, Fluorescent Antibody Technique, Gene Transfer Techniques, HeLa Cells, Humans, Immunoblotting, Mice, Transgenes, Transposases
Added August 22, 2013
0 Communities
2 Members
0 Resources
10 MeSH Terms
Comparative analysis of the recently discovered hAT transposon TcBuster in human cells.
Woodard LE, Li X, Malani N, Kaja A, Hice RH, Atkinson PW, Bushman FD, Craig NL, Wilson MH
(2012) PLoS One 7: e42666
MeSH Terms: Animals, Base Sequence, DNA Primers, DNA Transposable Elements, Gene Transfer Techniques, Genetic Engineering, HEK293 Cells, Humans, INDEL Mutation, Molecular Sequence Data, Mutagenesis, Insertional, Plasmids, Transposases, Tribolium
Added August 22, 2013
0 Communities
2 Members
0 Resources
14 MeSH Terms
piggyBac transposon system modification of primary human T cells.
Saha S, Nakazawa Y, Huye LE, Doherty JE, Galvan DL, Rooney CM, Wilson MH
(2012) J Vis Exp : e4235
MeSH Terms: Antibodies, CD28 Antigens, CD3 Complex, DNA Transposable Elements, Gene Transfer Techniques, Humans, Lymphocyte Activation, Plasmids, T-Lymphocytes, Cytotoxic, Transposases
Added August 22, 2013
0 Communities
1 Members
0 Resources
10 MeSH Terms
Hyperactive piggyBac gene transfer in human cells and in vivo.
Doherty JE, Huye LE, Yusa K, Zhou L, Craig NL, Wilson MH
(2012) Hum Gene Ther 23: 311-20
MeSH Terms: Animals, DNA Transposable Elements, Female, Gene Transfer Techniques, Genetic Vectors, HEK293 Cells, HeLa Cells, Humans, Mice, Transposases
Added August 22, 2013
0 Communities
1 Members
0 Resources
10 MeSH Terms
Manipulating piggyBac transposon chromosomal integration site selection in human cells.
Kettlun C, Galvan DL, George AL, Kaja A, Wilson MH
(2011) Mol Ther 19: 1636-44
MeSH Terms: Blotting, Western, Chromatin Immunoprecipitation, Chromosomes, Human, DNA Transposable Elements, DNA-Binding Proteins, Genetic Engineering, Genetic Vectors, Genome, Human, HEK293 Cells, Humans, Mutagenesis, Site-Directed, Plasmids, Real-Time Polymerase Chain Reaction, Transfection, Transposases, Zinc Fingers
Added August 22, 2013
0 Communities
1 Members
0 Resources
16 MeSH Terms
Designing and testing chimeric zinc finger transposases.
Wilson MH, George AL
(2010) Methods Mol Biol 649: 353-63
MeSH Terms: Blotting, Western, Cell Line, DNA Transposable Elements, Humans, Models, Genetic, Models, Theoretical, Mutagenesis, Site-Directed, Transposases, Zinc Fingers
Added August 22, 2013
0 Communities
1 Members
0 Resources
9 MeSH Terms
piggyBac transposon/transposase system to generate CD19-specific T cells for the treatment of B-lineage malignancies.
Manuri PV, Wilson MH, Maiti SN, Mi T, Singh H, Olivares S, Dawson MJ, Huls H, Lee DA, Rao PH, Kaminski JM, Nakazawa Y, Gottschalk S, Kebriaei P, Shpall EJ, Champlin RE, Cooper LJ
(2010) Hum Gene Ther 21: 427-37
MeSH Terms: Antigen-Presenting Cells, Antigens, CD19, Cell Line, Tumor, Cells, Cultured, Coculture Techniques, DNA Transposable Elements, Electroporation, Genetic Therapy, Genetic Vectors, Glioblastoma, Humans, K562 Cells, Lymphoma, B-Cell, Plasmids, Receptors, Antigen, T-Lymphocytes, Transgenes, Transposases
Added August 22, 2013
0 Communities
1 Members
0 Resources
18 MeSH Terms