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A recommended and verified procedure for in situ tryptic digestion of formalin-fixed paraffin-embedded tissues for analysis by matrix-assisted laser desorption/ionization imaging mass spectrometry.
Judd AM, Gutierrez DB, Moore JL, Patterson NH, Yang J, Romer CE, Norris JL, Caprioli RM
(2019) J Mass Spectrom 54: 716-727
MeSH Terms: Formaldehyde, Humans, Paraffin Embedding, Proteins, Proteolysis, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Tissue Array Analysis, Tissue Fixation, Trypsin
Added October 15, 2019
0 Communities
2 Members
0 Resources
MeSH Terms
Novel kidney dissociation protocol and image-based flow cytometry facilitate improved analysis of injured proximal tubules.
Manolopoulou M, Matlock BK, Nlandu-Khodo S, Simmons AJ, Lau KS, Phillips-Mignemi M, Ivanova A, Alford CE, Flaherty DK, Gewin LS
(2019) Am J Physiol Renal Physiol 316: F847-F855
MeSH Terms: Acute Kidney Injury, Animals, Aristolochic Acids, Biomarkers, Cell Cycle, Cell Separation, Disease Models, Animal, Epithelial Cells, Flow Cytometry, Genes, Reporter, Kidney Tubules, Proximal, Luminescent Proteins, Male, Mice, Transgenic, Polyploidy, Tissue Fixation
Added March 26, 2019
0 Communities
1 Members
0 Resources
16 MeSH Terms
Single-Cell Mass Cytometry of Archived Human Epithelial Tissue for Decoding Cancer Signaling Pathways.
Scurrah CR, Simmons AJ, Lau KS
(2019) Methods Mol Biol 1884: 215-229
MeSH Terms: Animals, Cryopreservation, Epithelial Cells, Epithelium, Fixatives, Flow Cytometry, Formaldehyde, Humans, Mass Spectrometry, Mice, Neoplasms, Paraffin Embedding, Signal Transduction, Single-Cell Analysis, Tissue Fixation
Added December 14, 2018
2 Communities
0 Members
0 Resources
15 MeSH Terms
Comparison of triple-negative breast cancer molecular subtyping using RNA from matched fresh-frozen versus formalin-fixed paraffin-embedded tissue.
Jovanović B, Sheng Q, Seitz RS, Lawrence KD, Morris SW, Thomas LR, Hout DR, Schweitzer BL, Guo Y, Pietenpol JA, Lehmann BD
(2017) BMC Cancer 17: 241
MeSH Terms: Female, Formaldehyde, Gene Expression Regulation, Neoplastic, High-Throughput Nucleotide Sequencing, Humans, Neoplasm Proteins, Paraffin Embedding, RNA, Tissue Fixation, Triple Negative Breast Neoplasms
Added April 9, 2017
1 Communities
1 Members
0 Resources
10 MeSH Terms
Evaluation of frozen tissue-derived prognostic gene expression signatures in FFPE colorectal cancer samples.
Zhu J, Deane NG, Lewis KB, Padmanabhan C, Washington MK, Ciombor KK, Timmers C, Goldberg RM, Beauchamp RD, Chen X
(2016) Sci Rep 6: 33273
MeSH Terms: Colorectal Neoplasms, Disease-Free Survival, Female, Formaldehyde, Gene Expression Regulation, Neoplastic, Humans, Male, Neoplasm Recurrence, Local, Oligonucleotide Array Sequence Analysis, Paraffin Embedding, Prognosis, Tissue Fixation, Transcriptome
Added November 12, 2016
1 Communities
1 Members
0 Resources
13 MeSH Terms
Comparison of Nanostring nCounter® Data on FFPE Colon Cancer Samples and Affymetrix Microarray Data on Matched Frozen Tissues.
Chen X, Deane NG, Lewis KB, Li J, Zhu J, Washington MK, Beauchamp RD
(2016) PLoS One 11: e0153784
MeSH Terms: Colonic Neoplasms, Formaldehyde, Frozen Sections, Gene Expression Regulation, Neoplastic, Humans, Oligonucleotide Array Sequence Analysis, Paraffin Embedding, RNA, Neoplasm, Survival Analysis, Tissue Fixation
Added August 10, 2016
1 Communities
1 Members
0 Resources
10 MeSH Terms
Whole-body tissue stabilization and selective extractions via tissue-hydrogel hybrids for high-resolution intact circuit mapping and phenotyping.
Treweek JB, Chan KY, Flytzanis NC, Yang B, Deverman BE, Greenbaum A, Lignell A, Xiao C, Cai L, Ladinsky MS, Bjorkman PJ, Fowlkes CC, Gradinaru V
(2015) Nat Protoc 10: 1860-1896
MeSH Terms: Animals, Detergents, Histocytochemistry, Lipids, Mice, Optical Imaging, Pathology, Rats, Specimen Handling, Staining and Labeling, Time Factors, Tissue Embedding, Tissue Fixation
Added July 20, 2016
1 Communities
0 Members
0 Resources
13 MeSH Terms
Histology-guided protein digestion/extraction from formalin-fixed and paraffin-embedded pressure ulcer biopsies.
Taverna D, Pollins AC, Nanney LB, Sindona G, Caprioli RM
(2016) Exp Dermatol 25: 143-6
MeSH Terms: Biopsy, Chromatography, Liquid, Eosine Yellowish-(YS), Formaldehyde, Hematoxylin, Humans, Hydrogels, Paraffin Embedding, Pressure Ulcer, Proteins, Proteomics, Specimen Handling, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Staining and Labeling, Tandem Mass Spectrometry, Tissue Fixation, Trypsin
Added October 15, 2015
1 Communities
1 Members
0 Resources
17 MeSH Terms
Loss of heterozygosity at the CYP2D6 locus in breast cancer: implications for germline pharmacogenetic studies.
Goetz MP, Sun JX, Suman VJ, Silva GO, Perou CM, Nakamura Y, Cox NJ, Stephens PJ, Miller VA, Ross JS, Chen D, Safgren SL, Kuffel MJ, Ames MM, Kalari KR, Gomez HL, Gonzalez-Angulo AM, Burgues O, Brauch HB, Ingle JN, Ratain MJ, Yelensky R
(2014) J Natl Cancer Inst 107:
MeSH Terms: Adult, Aged, Antineoplastic Agents, Hormonal, Biomarkers, Tumor, Breast Neoplasms, Cytochrome P-450 CYP2D6, DNA, Neoplasm, Disease-Free Survival, Female, Formaldehyde, Genotype, Humans, Loss of Heterozygosity, Middle Aged, Mouth Mucosa, Paraffin Embedding, Receptor, ErbB-2, Receptors, Estrogen, Survival Analysis, Tamoxifen, Tissue Fixation
Added February 22, 2016
0 Communities
1 Members
0 Resources
21 MeSH Terms
Validation of a molecular and pathological model for five-year mortality risk in patients with early stage lung adenocarcinoma.
Bueno R, Hughes E, Wagner S, Gutin AS, Lanchbury JS, Zheng Y, Archer MA, Gustafson C, Jones JT, Rushton K, Saam J, Kim E, Barberis M, Wistuba I, Wenstrup RJ, Wallace WA, Hartman AR, Harrison DJ
(2015) J Thorac Oncol 10: 67-73
MeSH Terms: Adenocarcinoma, Adenocarcinoma of Lung, Aged, Female, Formaldehyde, Humans, Lung Neoplasms, Male, Neoplasm Staging, Paraffin Embedding, Prognosis, Real-Time Polymerase Chain Reaction, Tissue Fixation
Added February 3, 2018
0 Communities
1 Members
0 Resources
13 MeSH Terms