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Isotopically nonstationary C flux analysis of cyanobacterial isobutyraldehyde production.
Jazmin LJ, Xu Y, Cheah YE, Adebiyi AO, Johnson CH, Young JD
(2017) Metab Eng 42: 9-18
MeSH Terms: Aldehydes, Bacterial Proteins, Carbon Isotopes, Malate Dehydrogenase, Phosphoenolpyruvate Carboxylase, Pyruvate Kinase, Staining and Labeling, Synechococcus
Show Abstract · Added September 11, 2017
We applied isotopically nonstationary C metabolic flux analysis (INST-MFA) to compare the pathway fluxes of wild-type (WT) Synechococcus elongatus PCC 7942 to an engineered strain (SA590) that produces isobutyraldehyde (IBA). The flux maps revealed a potential bottleneck at the pyruvate kinase (PK) reaction step that was associated with diversion of flux into a three-step PK bypass pathway involving the enzymes PEP carboxylase (PEPC), malate dehydrogenase (MDH), and malic enzyme (ME). Overexpression of pk in SA590 led to a significant improvement in IBA specific productivity. Single-gene overexpression of the three enzymes in the proposed PK bypass pathway also led to improvements in IBA production, although to a lesser extent than pk overexpression. Combinatorial overexpression of two of the three genes in the proposed PK bypass pathway (mdh and me) led to improvements in specific productivity that were similar to those achieved by single-gene pk overexpression. Our work demonstrates how C flux analysis can be used to identify potential metabolic bottlenecks and novel metabolic routes, and how these findings can guide rational metabolic engineering of cyanobacteria for increased production of desired molecules.
Copyright © 2017 International Metabolic Engineering Society. Published by Elsevier Inc. All rights reserved.
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1 Members
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8 MeSH Terms
An arginine tetrad as mediator of input-dependent and input-independent ATPases in the clock protein KaiC.
Pattanayek R, Xu Y, Lamichhane A, Johnson CH, Egli M
(2014) Acta Crystallogr D Biol Crystallogr 70: 1375-90
MeSH Terms: Adenosine Triphosphatases, Arginine, Bacterial Proteins, Binding Sites, Circadian Rhythm Signaling Peptides and Proteins, Crystallography, X-Ray, Models, Molecular, Mutagenesis, Site-Directed, Phosphorylation, Protein Structure, Tertiary, Synechococcus
Show Abstract · Added May 27, 2014
A post-translational oscillator (PTO) composed of the proteins KaiA, KaiB and KaiC is at the heart of the cyanobacterial circadian clock. KaiC interacts with KaiA and KaiB over the daily cycle, and CII domains undergo rhythmic phosphorylation/dephosphorylation with a 24 h period. Both the N-terminal (CI) and C-terminal (CII) rings of KaiC exhibit ATPase activity. The CI ATPase proceeds in an input-independent fashion, but the CII ATPase is subject to metabolic input signals. The crystal structure of KaiC from Thermosynechococcus elongatus allows insight into the different anatomies of the CI and CII ATPases. Four consecutive arginines in CI (Arg linker) that connect the P-loop, CI subunits and CI and CII at the ring interface are primary candidates for the coordination of the CI and CII activities. The mutation of linker residues alters the period or triggers arhythmic behavior. Comparison between the CI and CII structures also reveals differences in loop regions that are key to KaiA and KaiB binding and activation of CII ATPase and kinase. Common packing features in KaiC crystals shed light on the KaiB-KaiC interaction.
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2 Members
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11 MeSH Terms
Circadian yin-yang regulation and its manipulation to globally reprogram gene expression.
Xu Y, Weyman PD, Umetani M, Xiong J, Qin X, Xu Q, Iwasaki H, Johnson CH
(2013) Curr Biol 23: 2365-74
MeSH Terms: Bacterial Proteins, CLOCK Proteins, Circadian Rhythm, Circadian Rhythm Signaling Peptides and Proteins, Gene Expression, Gene Expression Profiling, Gene Expression Regulation, Bacterial, Hydrogen, Hydrogenase, Multigene Family, Phosphorylation, Promoter Regions, Genetic, Synechococcus, Transcription, Genetic
Show Abstract · Added February 12, 2015
BACKGROUND - The cyanobacterial circadian program exerts genome-wide control of gene expression. KaiC undergoes rhythms of phosphorylation that are regulated by interactions with KaiA and KaiB. The phosphorylation status of KaiC is thought to mediate global transcription via output factors SasA, CikA, LabA, RpaA, and RpaB. Overexpression of kaiC has been reported to globally repress gene expression.
RESULTS - Here, we show that the positive circadian component KaiA upregulates "subjective dusk" genes and that its overexpression deactivates rhythmic gene expression without significantly affecting growth rates in constant light. We analyze the global patterns of expression that are regulated by KaiA versus KaiC and find in contrast to the previous report of KaiC repression that there is a "yin-yang" regulation of gene expression whereby kaiA overexpression activates "dusk genes" and represses "dawn genes," whereas kaiC overexpression complementarily activates dawn genes and represses dusk genes. Moreover, continuous induction of kaiA latched KaiABC-regulated gene expression to provide constitutively increased transcript levels of diverse endogenous and heterologous genes that are expressed in the predominant subjective dusk phase. In addition to analyzing KaiA regulation of endogenous gene expression, we apply these insights to the expression of heterologous proteins whose products are of potential value, namely human proinsulin, foreign luciferase, and exogenous hydrogenase.
CONCLUSIONS - Both KaiC and KaiA complementarily contribute to the regulation of circadian gene expression via yin-yang switching. Circadian patterns can be reprogrammed by overexpression of kaiA or kaiC to constitutively enhance gene expression, and this reprogramming can improve 24/7 production of heterologous proteins that are useful as pharmaceuticals or biofuels.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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1 Members
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14 MeSH Terms
CryoEM and molecular dynamics of the circadian KaiB-KaiC complex indicates that KaiB monomers interact with KaiC and block ATP binding clefts.
Villarreal SA, Pattanayek R, Williams DR, Mori T, Qin X, Johnson CH, Egli M, Stewart PL
(2013) J Mol Biol 425: 3311-24
MeSH Terms: Adenosine Triphosphate, Bacterial Proteins, Binding Sites, Circadian Rhythm Signaling Peptides and Proteins, Cryoelectron Microscopy, Crystallography, X-Ray, Models, Molecular, Molecular Dynamics Simulation, Multiprotein Complexes, Phosphorylation, Protein Binding, Protein Multimerization, Protein Structure, Quaternary, Synechococcus
Show Abstract · Added March 7, 2014
The circadian control of cellular processes in cyanobacteria is regulated by a posttranslational oscillator formed by three Kai proteins. During the oscillator cycle, KaiA serves to promote autophosphorylation of KaiC while KaiB counteracts this effect. Here, we present a crystallographic structure of the wild-type Synechococcus elongatus KaiB and a cryo-electron microscopy (cryoEM) structure of a KaiBC complex. The crystal structure shows the expected dimer core structure and significant conformational variations of the KaiB C-terminal region, which is functionally important in maintaining rhythmicity. The KaiBC sample was formed with a C-terminally truncated form of KaiC, KaiC-Δ489, which is persistently phosphorylated. The KaiB-KaiC-Δ489 structure reveals that the KaiC hexamer can bind six monomers of KaiB, which form a continuous ring of density in the KaiBC complex. We performed cryoEM-guided molecular dynamics flexible fitting simulations with crystal structures of KaiB and KaiC to probe the KaiBC protein-protein interface. This analysis indicated a favorable binding mode for the KaiB monomer on the CII end of KaiC, involving two adjacent KaiC subunits and spanning an ATP binding cleft. A KaiC mutation, R468C, which has been shown to affect the affinity of KaiB for KaiC and lengthen the period in a bioluminescence rhythm assay, is found within the middle of the predicted KaiBC interface. The proposed KaiB binding mode blocks access to the ATP binding cleft in the CII ring of KaiC, which provides insight into how KaiB might influence the phosphorylation status of KaiC.
Copyright © 2013 Elsevier Ltd. All rights reserved.
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1 Members
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14 MeSH Terms
Non-optimal codon usage is a mechanism to achieve circadian clock conditionality.
Xu Y, Ma P, Shah P, Rokas A, Liu Y, Johnson CH
(2013) Nature 495: 116-20
MeSH Terms: Bacterial Proteins, Circadian Clocks, Circadian Rhythm, Circadian Rhythm Signaling Peptides and Proteins, Codon, Gene Expression Regulation, Bacterial, Genes, Bacterial, Multigene Family, Phenotype, Selection, Genetic, Synechococcus, Temperature
Show Abstract · Added May 30, 2014
Circadian rhythms are oscillations in biological processes that function as a key adaptation to the daily rhythms of most environments. In the model cyanobacterial circadian clock system, the core oscillator proteins are encoded by the gene cluster kaiABC. Genes with high expression and functional importance, such as the kai genes, are usually encoded by optimal codons, yet the codon-usage bias of the kaiBC genes is not optimized for translational efficiency. We discovered a relationship between codon usage and a general property of circadian rhythms called conditionality; namely, that endogenous rhythmicity is robustly expressed under some environmental conditions but not others. Despite the generality of circadian conditionality, however, its molecular basis is unknown for any system. Here we show that in the cyanobacterium Synechococcus elongate, non-optimal codon usage was selected as a post-transcriptional mechanism to switch between circadian and non-circadian regulation of gene expression as an adaptive response to environmental conditions. When the kaiBC sequence was experimentally optimized to enhance expression of the KaiB and KaiC proteins, intrinsic rhythmicity was enhanced at cool temperatures that are experienced by this organism in its natural habitat. However, fitness at those temperatures was highest in cells in which the endogenous rhythms were suppressed at cool temperatures as compared with cells exhibiting high-amplitude rhythmicity. These results indicate natural selection against circadian systems in cyanobacteria that are intrinsically robust at cool temperatures. Modulation of circadian amplitude is therefore crucial to its adaptive significance. Moreover, these results show the direct effects of codon usage on a complex phenotype and organismal fitness. Our work also challenges the long-standing view of directional selection towards optimal codons, and provides a key example of natural selection against optimal codons to achieve adaptive responses to environmental changes.
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12 MeSH Terms
Nature of KaiB-KaiC binding in the cyanobacterial circadian oscillator.
Pattanayek R, Yadagiri KK, Ohi MD, Egli M
(2013) Cell Cycle 12: 810-7
MeSH Terms: Bacterial Proteins, Circadian Clocks, Circadian Rhythm Signaling Peptides and Proteins, Histidine, Models, Molecular, Nanostructures, Negative Staining, Oligopeptides, Protein Binding, Scattering, Small Angle, Static Electricity, Synechococcus, X-Ray Diffraction
Show Abstract · Added March 7, 2014
In the cyanobacteria Synechococcus elongatus and Thermosynechococcus elongatus, the KaiA, KaiB and KaiC proteins in the presence of ATP generate a post-translational oscillator (PTO) that can be reconstituted in vitro. KaiC is the result of a gene duplication and resembles a double doughnut with N-terminal CI and C-terminal CII hexameric rings. Six ATPs are bound between subunits in both the CI and CII ring. CI harbors ATPase activity, and CII catalyzes phosphorylation and dephosphorylation at T432 and S431 with a ca. 24-h period. KaiA stimulates KaiC phosphorylation, and KaiB promotes KaiC subunit exchange and sequesters KaiA on the KaiB-KaiC interface in the final stage of the clock cycle. Studies of the PTO protein-protein interactions are convergent in terms of KaiA binding to CII but have led to two opposing models of the KaiB-KaiC interaction. Electron microscopy (EM) and small angle X-ray scattering (SAXS), together with native PAGE using full-length proteins and separate CI and CII rings, are consistent with binding of KaiB to CII. Conversely, NMR together with gel filtration chromatography and denatured PAGE using monomeric CI and CII domains support KaiB binding to CI. To resolve the existing controversy, we studied complexes between KaiB and gold-labeled, full-length KaiC with negative stain EM. The EM data clearly demonstrate that KaiB contacts the CII ring. Together with the outcomes of previous analyses, our work establishes that only CII participates in interactions with KaiA and KaiB as well as with the His kinase SasA involved in the clock output pathway.
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13 MeSH Terms
Loop-loop interactions regulate KaiA-stimulated KaiC phosphorylation in the cyanobacterial KaiABC circadian clock.
Egli M, Pattanayek R, Sheehan JH, Xu Y, Mori T, Smith JA, Johnson CH
(2013) Biochemistry 52: 1208-20
MeSH Terms: Bacterial Proteins, Circadian Clocks, Circadian Rhythm Signaling Peptides and Proteins, Crystallography, X-Ray, Models, Molecular, Molecular Dynamics Simulation, Mutation, Phosphorylation, Protein Conformation, Protein Multimerization, Synechococcus, Thermodynamics, Valine
Show Abstract · Added May 30, 2013
The Synechococcus elongatus KaiA, KaiB, and KaiC proteins in the presence of ATP generate a post-translational oscillator that runs in a temperature-compensated manner with a period of 24 h. KaiA dimer stimulates phosphorylation of KaiC hexamer at two sites per subunit, T432 and S431, and KaiB dimers antagonize KaiA action and induce KaiC subunit exchange. Neither the mechanism of KaiA-stimulated KaiC phosphorylation nor that of KaiB-mediated KaiC dephosphorylation is understood in detail at present. We demonstrate here that the A422V KaiC mutant sheds light on the former mechanism. It was previously reported that A422V is less sensitive to dark pulse-induced phase resetting and has a reduced amplitude of the KaiC phosphorylation rhythm in vivo. A422 maps to a loop (422-loop) that continues toward the phosphorylation sites. By pulling on the C-terminal peptide of KaiC (A-loop), KaiA removes restraints from the adjacent 422-loop whose increased flexibility indirectly promotes kinase activity. We found in the crystal structure that A422V KaiC lacks phosphorylation at S431 and exhibits a subtle, local conformational change relative to wild-type KaiC. Molecular dynamics simulations indicate higher mobility of the 422-loop in the absence of the A-loop and mobility differences in other areas associated with phosphorylation activity between wild-type and mutant KaiCs. The A-loop-422-loop relay that informs KaiC phosphorylation sites of KaiA dimer binding propagates to loops from neighboring KaiC subunits, thus providing support for a concerted allosteric mechanism of phosphorylation.
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3 Members
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13 MeSH Terms
Combined SAXS/EM based models of the S. elongatus post-translational circadian oscillator and its interactions with the output His-kinase SasA.
Pattanayek R, Williams DR, Rossi G, Weigand S, Mori T, Johnson CH, Stewart PL, Egli M
(2011) PLoS One 6: e23697
MeSH Terms: Bacterial Proteins, Binding Sites, Circadian Clocks, Circadian Rhythm Signaling Peptides and Proteins, Crystallography, X-Ray, Histidine Kinase, Microscopy, Electron, Phosphotransferases, Protein Conformation, Protein Interaction Domains and Motifs, Protein Kinases, Scattering, Small Angle, Synechococcus, X-Ray Diffraction
Show Abstract · Added March 7, 2014
The circadian clock in the cyanobacterium Synechococcus elongatus is composed of a post-translational oscillator (PTO) that can be reconstituted in vitro from three different proteins in the presence of ATP and a transcription-translation feedback loop (TTFL). The homo-hexameric KaiC kinase, phosphatase and ATPase alternates between hypo- and hyper-phosphorylated states over the 24-h cycle, with KaiA enhancing phosphorylation, and KaiB antagonizing KaiA and promoting KaiC subunit exchange. SasA is a His kinase that relays output signals from the PTO formed by the three Kai proteins to the TTFL. Although the crystal structures for all three Kai proteins are known, atomic resolution structures of Kai and Kai/SasA protein complexes have remained elusive. Here, we present models of the KaiAC and KaiBC complexes derived from solution small angle X-ray scattering (SAXS), which are consistent with previous EM based models. We also present a combined SAXS/EM model of the KaiC/SasA complex, which has two N-terminal SasA sensory domains occupying positions on the C-terminal KaiC ring reminiscent of the orientations adopted by KaiB dimers. Using EM we demonstrate that KaiB and SasA compete for similar binding sites on KaiC. We also propose an EM based model of the ternary KaiABC complex that is consistent with the sequestering of KaiA by KaiB on KaiC during the PTO dephosphorylation phase. This work provides the first 3D-catalogue of protein-protein interactions in the KaiABC PTO and the output pathway mediated by SasA.
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2 Members
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14 MeSH Terms
Intermolecular associations determine the dynamics of the circadian KaiABC oscillator.
Qin X, Byrne M, Mori T, Zou P, Williams DR, McHaourab H, Johnson CH
(2010) Proc Natl Acad Sci U S A 107: 14805-10
MeSH Terms: Algorithms, Bacterial Proteins, Circadian Rhythm, Circadian Rhythm Signaling Peptides and Proteins, Electrophoresis, Polyacrylamide Gel, Fluorescence Polarization, Kinetics, Models, Biological, Models, Chemical, Molecular Dynamics Simulation, Mutation, Peptides, Phosphorylation, Protein Binding, Synechococcus
Show Abstract · Added February 12, 2015
Three proteins from cyanobacteria (KaiA, KaiB, and KaiC) can reconstitute circadian oscillations in vitro. At least three molecular properties oscillate during this reaction, namely rhythmic phosphorylation of KaiC, ATP hydrolytic activity of KaiC, and assembly/disassembly of intermolecular complexes among KaiA, KaiB, and KaiC. We found that the intermolecular associations determine key dynamic properties of this in vitro oscillator. For example, mutations within KaiB that alter the rates of binding of KaiB to KaiC also predictably modulate the period of the oscillator. Moreover, we show that KaiA can bind stably to complexes of KaiB and hyperphosphorylated KaiC. Modeling simulations indicate that the function of this binding of KaiA to the KaiB*KaiC complex is to inactivate KaiA's activity, thereby promoting the dephosphorylation phase of the reaction. Therefore, we report here dynamics of interaction of KaiA and KaiB with KaiC that determine the period and amplitude of this in vitro oscillator.
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1 Members
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15 MeSH Terms
Structures of KaiC circadian clock mutant proteins: a new phosphorylation site at T426 and mechanisms of kinase, ATPase and phosphatase.
Pattanayek R, Mori T, Xu Y, Pattanayek S, Johnson CH, Egli M
(2009) PLoS One 4: e7529
MeSH Terms: Adenosine Triphosphatases, Adenosine Triphosphate, Bacterial Proteins, Binding Sites, Catalytic Domain, Circadian Rhythm, Circadian Rhythm Signaling Peptides and Proteins, Crystallography, X-Ray, Gene Expression Regulation, Bacterial, Models, Biological, Mutation, Phosphoric Monoester Hydrolases, Phosphorylation, Phosphotransferases, Synechococcus
Show Abstract · Added May 27, 2014
BACKGROUND - The circadian clock of the cyanobacterium Synechococcus elongatus can be reconstituted in vitro by three proteins, KaiA, KaiB and KaiC. Homo-hexameric KaiC displays kinase, phosphatase and ATPase activities; KaiA enhances KaiC phosphorylation and KaiB antagonizes KaiA. Phosphorylation and dephosphorylation of the two known sites in the C-terminal half of KaiC subunits, T432 and S431, follow a strict order (TS-->pTS-->pTpS-->TpS-->TS) over the daily cycle, the origin of which is not understood. To address this void and to analyze the roles of KaiC active site residues, in particular T426, we determined structures of single and double P-site mutants of S. elongatus KaiC.
METHODOLOGY AND PRINCIPAL FINDINGS - The conformations of the loop region harboring P-site residues T432 and S431 in the crystal structures of six KaiC mutant proteins exhibit subtle differences that result in various distances between Thr (or Ala/Asn/Glu) and Ser (or Ala/Asp) residues and the ATP gamma-phosphate. T432 is phosphorylated first because it lies consistently closer to Pgamma. The structures of the S431A and T432E/S431A mutants reveal phosphorylation at T426. The environments of the latter residue in the structures and functional data for T426 mutants in vitro and in vivo imply a role in dephosphorylation.
CONCLUSIONS AND SIGNIFICANCE - We provide evidence for a third phosphorylation site in KaiC at T426. T426 and S431 are closely spaced and a KaiC subunit cannot carry phosphates at both sites simultaneously. Fewer subunits are phosphorylated at T426 in the two KaiC mutants compared to phosphorylated T432 and/or S431 residues in the structures of wt and other mutant KaiCs, suggesting that T426 phosphorylation may be labile. The structures combined with functional data for a host of KaiC mutant proteins help rationalize why S431 trails T432 in the loss of its phosphate and shed light on the mechanisms of the KaiC kinase, ATPase and phosphatase activities.
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15 MeSH Terms