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Results: 1 to 3 of 3

Publication Record


Fluid management in cardiac surgery: colloid or crystalloid?
Shaw A, Raghunathan K
(2013) Anesthesiol Clin 31: 269-80
MeSH Terms: Acute Kidney Injury, Atrial Fibrillation, Cardiac Surgical Procedures, Cardioplegic Solutions, Colloids, Crystalloid Solutions, Fluid Therapy, Humans, Isotonic Solutions, Postoperative Complications, Saline Solution, Hypertonic
Show Abstract · Added October 20, 2015
The crystalloid-colloid debate has raged for decades, with the publication of many meta-analyses, yet no consensus. There are important differences between colloids and crystalloids, and these differences have direct relevance for cardiac surgical patients. Rather than asking crystalloid or colloid, we believe better questions to ask are (1) High or low chloride content? and (2) Synthetic or natural colloid? In this paper we review the published literature regarding fluid therapy in cardiac surgery and explain the background to these two important and unanswered questions.
Published by Elsevier Inc.
0 Communities
1 Members
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11 MeSH Terms
Molecular mechanisms of stress-induced proenkephalin gene regulation: CREB interacts with the proenkephalin gene in the mouse hypothalamus and is phosphorylated in response to hyperosmolar stress.
Borsook D, Konradi C, Falkowski O, Comb M, Hyman SE
(1994) Mol Endocrinol 8: 240-8
MeSH Terms: Animals, Base Sequence, Cyclic AMP Response Element-Binding Protein, Enkephalins, Gene Expression Regulation, Genes, fos, Hypothalamus, Male, Mice, Mice, Transgenic, Molecular Sequence Data, Oligonucleotides, Osmotic Pressure, Phosphorylation, Protein Precursors, Proto-Oncogene Proteins c-jun, Regulatory Sequences, Nucleic Acid, Saline Solution, Hypertonic, beta-Galactosidase
Show Abstract · Added May 27, 2014
We have established a transgenic model to facilitate the study of stress-induced gene regulation in the hypothalamus. This model, which uses a human proenkephalin-beta-galactosidase fusion gene, readily permits anatomic and cellular colocalization of stress-regulated immediate early gene products (e.g. Fos) and other transcription factors [e.g. cAMP response element-binding protein (CREB)] with the product of a potential target gene. Moreover, Fos provides a marker of cellular activation that is independent of the transgene. Hypertonic saline stress induced Fos in almost all cells in the PVN that exhibited basal expression of the proenkephalin transgene; however, all cells in which the transgene was activated by stress also expressed Fos. CREB was found in essentially all neurons. Gel shift analysis with and without antisera to Fos and CREB showed that AP-1 binding activity, containing Fos protein, was induced by hyperosmotic stress. However, Fos was not detected binding to the proenkephalin second messenger-inducible enhancer even in hypothalamic cell extracts from stressed animals. In contrast, CREB formed specific complexes with both the proenkephalin enhancer and a cAMP- and calcium-regulated element (CaRE) within the c-fos gene. Moreover, we found that hypertonic saline induced CREB phosphorylation in cells that express the transgene within the paraventricular nucleus and supraoptic nucleus. These results suggest a model in which proenkephalin gene expression in the paraventricular nucleus is regulated by CREB in response to hypertonic stress.
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19 MeSH Terms
Stress-induced regulation of a human proenkephalin-beta-galactosidase fusion gene in the hypothalamus of transgenic mice.
Borsook D, Falkowski O, Burstein R, Strassman A, Konradi C, Dauber A, Comb M, Hyman SE
(1994) Mol Endocrinol 8: 116-25
MeSH Terms: Animals, Blood Volume, Cold Temperature, Enkephalins, Gene Expression Regulation, Humans, Hypothalamus, In Situ Hybridization, Mice, Mice, Transgenic, Neurons, Paraventricular Hypothalamic Nucleus, Protein Precursors, RNA, Messenger, Recombinant Fusion Proteins, Saline Solution, Hypertonic, Stress, Physiological, Supraoptic Nucleus, Tissue Distribution, beta-Galactosidase
Show Abstract · Added May 27, 2014
Transgenic mice expressing an Escherichia coli beta-galactosidase reporter gene under the control of 3 kilobases of human proenkephalin gene 5'-flanking sequence and 1.2 kilobases of 3'-flanking sequence exhibited an anatomically correct pattern of basal and stress-regulated transgene expression within the hypothalamus. Acute osmotic stress and hypovolemia induced transgene expression in neurons within both the paraventricular and supraoptic nuclei. Chronic osmotic stress resulted in dramatic induction of transgene expression in both nuclei. These results demonstrate that the information required for correct hypothalamic expression and stress regulation of the proenkephalin gene is contained within our fusion construct.
0 Communities
1 Members
0 Resources
20 MeSH Terms