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Results: 1 to 10 of 21

Publication Record


Computational modelling of epidermal stratification highlights the importance of asymmetric cell division for predictable and robust layer formation.
Gord A, Holmes WR, Dai X, Nie Q
(2014) J R Soc Interface 11:
MeSH Terms: Cell Adhesion, Cell Communication, Cell Division, Computational Biology, Computer Simulation, Epidermal Cells, Epidermis, Models, Biological, Time Factors
Show Abstract · Added February 26, 2016
Skin is a complex organ tasked with, among other functions, protecting the body from the outside world. Its outermost protective layer, the epidermis, is comprised of multiple cell layers that are derived from a single-layered ectoderm during development. Using a new stochastic, multi-scale computational modelling framework, the anisotropic subcellular element method, we investigate the role of cell morphology and biophysical cell-cell interactions in the formation of this layered structure. This three-dimensional framework describes interactions between collections of hundreds to thousands of cells and (i) accounts for intracellular structure and morphology, (ii) easily incorporates complex cell-cell interactions and (iii) can be efficiently implemented on parallel architectures. We use this approach to construct a model of the developing epidermis that accounts for the internal polarity of ectodermal cells and their columnar morphology. Using this model, we show that cell detachment, which has been previously suggested to have a role in this process, leads to unpredictable, randomized stratification and that this cannot be abrogated by adjustment of cell-cell adhesion interaction strength. Polarized distribution of cell adhesion proteins, motivated by epithelial polarization, can however eliminate this detachment, and in conjunction with asymmetric cell division lead to robust and predictable development.
© 2014 The Author(s) Published by the Royal Society. All rights reserved.
0 Communities
1 Members
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9 MeSH Terms
Spatial and temporal in vivo analysis of circulating and sessile immune cells in mosquitoes: hemocyte mitosis following infection.
King JG, Hillyer JF
(2013) BMC Biol 11: 55
MeSH Terms: Aging, Animals, Anopheles, Carbocyanines, Cell Adhesion, Cell Count, Cytoskeleton, Epidermal Cells, Escherichia coli, Escherichia coli Infections, Flight, Animal, Hemocytes, Immunity, Lymphocytes, Mitosis, Muscles, Organ Specificity, Phagocytosis, Spatio-Temporal Analysis, Swimming
Show Abstract · Added February 5, 2016
BACKGROUND - Mosquitoes respond to infection by mounting immune responses. The primary regulators of these immune responses are cells called hemocytes, which kill pathogens via phagocytosis and via the production of soluble antimicrobial factors. Mosquito hemocytes are circulated throughout the hemocoel (body cavity) by the swift flow of hemolymph (blood), and data show that some hemocytes also exist as sessile cells that are attached to tissues. The purpose of this study was to create a quantitative physical map of hemocyte distribution in the mosquito, Anopheles gambiae, and to describe the cellular immune response in an organismal context.
RESULTS - Using correlative imaging methods we found that the number of hemocytes in a mosquito decreases with age, but that regardless of age, approximately 75% of the hemocytes occur in circulation and 25% occur as sessile cells. Infection induces an increase in the number of hemocytes, and tubulin and nuclear staining showed that this increase is primarily due to mitosis and, more specifically, autonomous cell division, by circulating granulocytes. The majority of sessile hemocytes are present on the abdominal wall, although significant numbers of hemocytes are also present in the thorax, head, and several of the appendages. Within the abdominal wall, the areas of highest hemocyte density are the periostial regions (regions surrounding the valves of the heart, or ostia), which are ideal locations for pathogen capture as these are areas of high hemolymph flow.
CONCLUSIONS - These data describe the spatial and temporal distribution of mosquito hemocytes, and map the cellular response to infection throughout the hemocoel.
0 Communities
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20 MeSH Terms
Artificial forisomes are ideal models of forisome assembly and activity that allow the development of technical devices.
Groscurth S, Müller B, Schwan S, Menzel M, Diekstall F, Senft M, Kendall A, Kommor BA, Neumann U, Kalischuk M, Kawchuk LM, Krzyzanek V, Heilmann A, Stubbs G, Twyman RM, Prüfer D, Noll GA
(2012) Biomacromolecules 13: 3076-86
MeSH Terms: Agrobacterium tumefaciens, Epidermal Cells, Epidermis, Medicago truncatula, Membranes, Artificial, Microscopy, Confocal, Microscopy, Electron, Transmission, Models, Molecular, Plant Proteins, Tobacco
Show Abstract · Added February 15, 2016
Forisomes are protein polymers found in leguminous plants that have the remarkable ability to undergo reversible "muscle-like" contractions in the presence of divalent cations and in extreme pH environments. To gain insight into the molecular basis of forisome structure and assembly, we used confocal laser scanning microscopy to monitor the assembly of fluorescence-labeled artificial forisomes in real time, revealing two distinct assembly processes involving either fiber elongation or fiber alignment. We also used scanning and transmission electron microscopy and X-ray diffraction to investigate the ultrastructure of forisomes, finding that individual fibers are arranged into compact fibril bundles that disentangle with minimal residual order in the presence of calcium ions. To demonstrate the potential applications of artificial forisomes, we created hybrid protein bodies from forisome subunits fused to the B-domain of staphylococcal protein A. This allowed the functionalization of the artificial forisomes with antibodies that were then used to target forisomes to specific regions on a substrate, providing a straightforward approach to develop forisome-based technical devices with precise configurations. The functional contractile properties of forisomes are also better preserved when they are immobilized via affinity reagents rather than by direct contact to the substrate. Artificial forisomes produced in plants and yeast therefore provide an ideal model for the investigation of forisome structure and assembly and for the design and testing of tailored artificial forisomes for technical applications.
0 Communities
1 Members
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10 MeSH Terms
Inducible deletion of epidermal Dicer and Drosha reveals multiple functions for miRNAs in postnatal skin.
Teta M, Choi YS, Okegbe T, Wong G, Tam OH, Chong MM, Seykora JT, Nagy A, Littman DR, Andl T, Millar SE
(2012) Development 139: 1405-16
MeSH Terms: Animals, Crosses, Genetic, DEAD-box RNA Helicases, Epidermal Cells, Gene Deletion, Hair Follicle, Mice, MicroRNAs, Microscopy, Fluorescence, Phenotype, Ribonuclease III, Signal Transduction, Skin, Stem Cells, Wound Healing
Show Abstract · Added January 30, 2013
MicroRNAs (miRNAs) regulate the expression of many mammalian genes and play key roles in embryonic hair follicle development; however, little is known of their functions in postnatal hair growth. We compared the effects of deleting the essential miRNA biogenesis enzymes Drosha and Dicer in mouse skin epithelial cells at successive postnatal time points. Deletion of either Drosha or Dicer during an established growth phase (anagen) caused failure of hair follicles to enter a normal catagen regression phase, eventual follicular degradation and stem cell loss. Deletion of Drosha or Dicer in resting phase follicles did not affect follicular structure or epithelial stem cell maintenance, and stimulation of anagen by hair plucking caused follicular proliferation and formation of a primitive transient amplifying matrix population. However, mutant matrix cells exhibited apoptosis and DNA damage and hair follicles rapidly degraded. Hair follicle defects at early time points post-deletion occurred in the absence of inflammation, but a dermal inflammatory response and hyperproliferation of interfollicular epidermis accompanied subsequent hair follicle degradation. These data reveal multiple functions for Drosha and Dicer in suppressing DNA damage in rapidly proliferating follicular matrix cells, facilitating catagen and maintaining follicular structures and their associated stem cells. Although Drosha and Dicer each possess independent non-miRNA-related functions, the similarity in phenotypes of the inducible epidermal Drosha and Dicer mutants indicates that these defects result primarily from failure of miRNA processing. Consistent with this, Dicer deletion resulted in the upregulation of multiple direct targets of the highly expressed epithelial miRNA miR-205.
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15 MeSH Terms
Role of epidermal primary cilia in the homeostasis of skin and hair follicles.
Croyle MJ, Lehman JM, O'Connor AK, Wong SY, Malarkey EB, Iribarne D, Dowdle WE, Schoeb TR, Verney ZM, Athar M, Michaud EJ, Reiter JF, Yoder BK
(2011) Development 138: 1675-85
MeSH Terms: Animals, Animals, Newborn, Cilia, Epidermal Cells, Epidermis, Gene Expression Regulation, Developmental, Hair Follicle, Homeostasis, Integrases, Kinesin, Mice, Mice, Inbred C57BL, Mice, Transgenic, Phenotype, Skin Physiological Phenomena, Transgenes, Tumor Suppressor Proteins
Show Abstract · Added March 31, 2020
Skin and hair follicle morphogenesis and homeostasis require the integration of multiple signaling pathways, including Hedgehog (Hh) and Wingless (Wnt), and oriented cell divisions, all of which have been associated with primary cilia. Although studies have shown that disrupting dermal cilia causes follicular arrest and attenuated Hh signaling, little is known about the role of epidermal cilia. Here, epidermal cilia function was analyzed using conditional alleles of the ciliogenic genes Ift88 and Kif3a. At birth, epidermal cilia mutants appeared normal, but developed basaloid hyperplasia and ingrowths into the dermis of the ventrum with age. In addition, follicles in the tail were disorganized and had excess sebaceous gland lobules. Epidermal cilia mutants displayed fewer long-term label-retaining cells, suggesting altered stem cell homeostasis. Abnormal proliferation and differentiation were evident from lineage-tracing studies and showed an expansion of follicular cells into the interfollicular epidermis, as is seen during wound repair. These phenotypes were not associated with changes in canonical Wnt activity or oriented cell division. However, nuclear accumulation of the ΔNp63 transcription factor, which is involved in stratification, keratinocyte differentiation and wound repair, was increased, whereas the Hh pathway was repressed. Intriguingly, the phenotypes were not typical of those associated with loss of Hh signaling but exhibited similarities with those of mice in which ΔNp63 is overexpressed in the epidermis. Collectively, these data indicate that epidermal primary cilia may function in stress responses and epidermal homeostasis involving pathways other than those typically associated with primary cilia.
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MeSH Terms
Lrig1 expression defines a distinct multipotent stem cell population in mammalian epidermis.
Jensen KB, Collins CA, Nascimento E, Tan DW, Frye M, Itami S, Watt FM
(2009) Cell Stem Cell 4: 427-39
MeSH Terms: Animals, Cell Differentiation, Cell Lineage, Cell Proliferation, Epidermal Cells, Epidermis, Hair Follicle, Mammals, Membrane Glycoproteins, Mice, Mice, Transgenic, Multipotent Stem Cells, Nerve Tissue Proteins, Proto-Oncogene Proteins c-myc, beta Catenin
Show Abstract · Added August 19, 2010
Lrig1 is a marker of human interfollicular epidermal stem cells and helps maintain stem cell quiescence. We show that, in mouse epidermis, Lrig1 defines the hair follicle junctional zone adjacent to the sebaceous glands and infundibulum. Lrig1 is a Myc target gene; loss of Lrig1 increases the proliferative capacity of stem cells in culture and results in epidermal hyperproliferation in vivo. Lrig1-expressing cells can give rise to all of the adult epidermal lineages in skin reconstitution assays. However, during homeostasis and on retinoic acid stimulation, they are bipotent, contributing to the sebaceous gland and interfollicular epidermis. beta-catenin activation increases the size of the junctional zone compartment, and loss of Lrig1 causes a selective increase in beta-catenin-induced ectopic hair follicle formation in the interfollicular epidermis. Our results suggest that Lrig1-positive cells constitute a previously unidentified reservoir of adult mouse interfollicular epidermal stem cells.
0 Communities
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15 MeSH Terms
Epidermal lipoxygenase products of the hepoxilin pathway selectively activate the nuclear receptor PPARalpha.
Yu Z, Schneider C, Boeglin WE, Brash AR
(2007) Lipids 42: 491-7
MeSH Terms: 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid, 8,11,14-Eicosatrienoic Acid, Arachidonate Lipoxygenases, Cell Differentiation, Cell Line, Epidermal Cells, Epidermis, Genes, Reporter, Humans, Hydroxyeicosatetraenoic Acids, Lipoxygenase, Luciferases, PPAR alpha, PPAR delta, PPAR gamma, Receptors, Cytoplasmic and Nuclear, Signal Transduction
Show Abstract · Added December 10, 2013
Arachidonic acid can be transformed into a specific epoxyalcohol product via the sequential action of two epidermal lipoxygenases, 12R-LOX and eLOX3. Functional impairment of either lipoxygenase gene (ALOX12B or ALOXE3) results in ichthyosis, suggesting a role for the common epoxyalcohol product or its metabolites in the differentiation of normal human skin. Here we tested the ability of products derived from the epidermal LOX pathway to activate the peroxisome proliferator-activated receptors PPARalpha, gamma, and delta, which have been implicated in epidermal differentiation. Using a dual luciferase reporter assay in PC3 cells, the 12R-LOX/eLOX3-derived epoxyalcohol, 8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid, activated PPARalpha with similar in potency to the known natural ligand, 8S-hydroxyeicosatetraenoic acid (8S-HETE) (both at 10 microM concentration). In contrast, the PPARgamma and PPARdelta receptor isoforms were not activated by the epoxyalcohol. Activation of PPARalpha was also observed using the trihydroxy hydrolysis products (trioxilins) of the unstable epoxyalcohol. Of the four trioxilins isolated and characterized, the highest activation was observed with the isomer that is also formed by enzymatic hydrolysis of the epoxyalcohol. Formation of a ligand for the nuclear receptor PPARalpha may be one possibility by which 12R-LOX and eLOX3 contribute to epidermal differentiation.
0 Communities
2 Members
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17 MeSH Terms
The role of proline-rich protein tyrosine kinase 2 in differentiation-dependent signaling in human epidermal keratinocytes.
Schindler EM, Baumgartner M, Gribben EM, Li L, Efimova T
(2007) J Invest Dermatol 127: 1094-106
MeSH Terms: Calcium, Cell Differentiation, Cell Nucleus, Cells, Cultured, Epidermal Cells, Epidermis, Focal Adhesion Kinase 2, Gene Expression Regulation, Humans, Indoles, Keratinocytes, Maleimides, Protein Kinase C, Protein Precursors, Proto-Oncogene Proteins c-fos, Proto-Oncogene Proteins c-jun, Signal Transduction, Tetradecanoylphorbol Acetate
Show Abstract · Added May 30, 2013
Non-receptor tyrosine kinase proline-rich protein tyrosine kinase 2 (Pyk2) functions as an integrator of multiple signaling pathways involved in the regulation of fundamental cellular processes. Pyk2 expression, regulation, and functions in skin have not been examined. Here we investigated the expression and subcellular localization of Pyk2 in human epidermis and in primary human keratinocytes, and studied the mechanisms of Pyk2 activation by differentiation-inducing stimuli, and the role of Pyk2 as a regulator of keratinocyte differentiation. We demonstrate that Pyk2 is abundantly expressed in skin keratinocytes. Notably, the endogenous Pyk2 protein is predominantly localized in keratinocyte nuclei throughout all layers of healthy human epidermis, and in cultured human keratinocytes. Pyk2 is activated by treatment with keratinocyte-differentiating agents, 12-O-tetradecanoylphorbol-13-acetate and calcium via a mechanism that requires intracellular calcium release and functional protein kinase C (PKC) and Src activities. Particularly, differentiation-promoting PKC delta and PKC eta elicit Pyk2 activation. Our data show that Pyk2 increases promoter activity and endogenous protein levels of involucrin, a marker of keratinocyte terminal differentiation. This regulation is associated with increased expression of Fra-1 and JunD, activator protein-1 transcription factors known to be required for involucrin expression. Altogether, these results provide insights into Pyk2 signaling in epidermis and reveal a novel role for Pyk2 in regulation of keratinocyte differentiation.
0 Communities
1 Members
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18 MeSH Terms
The requirement for perp in postnatal viability and epithelial integrity reflects an intrinsic role in stratified epithelia.
Marques MR, Ihrie RA, Horner JS, Attardi LD
(2006) J Invest Dermatol 126: 69-73
MeSH Terms: Animals, Cell Adhesion, Cell Proliferation, Epidermal Cells, Epidermis, Genes, Lethal, Heart, Keratin-15, Keratin-5, Keratins, Membrane Proteins, Mice, Mice, Knockout, Myocardium
Show Abstract · Added August 21, 2012
Mice lacking the desmosome protein Perp exhibit blistering in their stratified epithelia and display postnatal lethality. However, it is unclear if these phenotypes are strictly related to Perp function in stratified epithelia, as Perp expression is not restricted to these tissues during embryogenesis, and certain desmosomal blistering diseases such as pemphigus vulgaris and pemphigus foliaceus have non-cell-intrinsic bases. Furthermore, we show here that Perp is expressed in the heart, raising the possibility that defects in heart function could account for lethality in the Perp-deficient mice. To determine conclusively if Perp function in stratified epithelia is crucial for postnatal survival and epithelial adhesion, we specifically ablated Perp in stratified epithelia by breeding conditional Perp knockout mice to keratin 5 (K5)-Cre transgenic mice. We found that the majority of mice lacking Perp in stratified epithelia die within 10 days after birth, accompanied by blistering and hyperproliferation in the epithelia, similar to the constitutive Perp null mice. Together, these findings indicate that Perp's requirement for both viability and epithelial integrity reflects a role in the stratified epithelial compartment.
1 Communities
1 Members
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14 MeSH Terms
Bves expression during avian embryogenesis.
Osler ME, Bader DM
(2004) Dev Dyn 229: 658-67
MeSH Terms: Amino Acid Sequence, Animals, Avian Proteins, Blotting, Western, Cell Adhesion, Cell Adhesion Molecules, Chick Embryo, DNA Primers, Epidermal Cells, Epithelium, Gastrula, Gene Expression Regulation, Developmental, Immunohistochemistry, Intestines, Molecular Sequence Data, Muscle Proteins, Muscles, Reverse Transcriptase Polymerase Chain Reaction, Skin, Time Factors
Show Abstract · Added September 28, 2015
Bves (blood vessel/epicardial substance) is a transmembrane protein postulated to play a role in cell adhesion. While it is clear that Bves and gene products of the same family are expressed in adult striated muscle cells, the distribution of these proteins during development has not been critically examined. An understanding of the expression pattern of Bves is essential for a determination of protein function and its role in embryogenesis. In this study, we present an expression analysis of Bves during chick gastrulation and germ layer formation. Our data show that Bves is expressed in epithelia of all three germ layers early in development. Furthermore, Bves protein is observed in epithelial tissues during organogenesis, specifically the developing epidermis, the gut endoderm, and the epicardium of the heart. These data support the hypothesis that Bves may play a role in cell adhesion and movement of epithelia during early embryogenesis.
Copyright 2004 Wiley-Liss, Inc.
1 Communities
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20 MeSH Terms