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Neuron-specific deletion of peroxisome proliferator-activated receptor delta (PPARδ) in mice leads to increased susceptibility to diet-induced obesity.
Kocalis HE, Turney MK, Printz RL, Laryea GN, Muglia LJ, Davies SS, Stanwood GD, McGuinness OP, Niswender KD
(2012) PLoS One 7: e42981
MeSH Terms: Animals, Body Composition, Brain, Diet, Fat-Restricted, Diet, High-Fat, Eating, Energy Metabolism, Female, Lipid Metabolism, Male, Mice, Mice, Knockout, Obesity, PPAR delta
Show Abstract · Added December 10, 2013
Central nervous system (CNS) lipid accumulation, inflammation and resistance to adipo-regulatory hormones, such as insulin and leptin, are implicated in the pathogenesis of diet-induced obesity (DIO). Peroxisome proliferator-activated receptors (PPAR α, δ, γ) are nuclear transcription factors that act as environmental fatty acid sensors and regulate genes involved in lipid metabolism and inflammation in response to dietary and endogenous fatty acid ligands. All three PPAR isoforms are expressed in the CNS at different levels. Recent evidence suggests that activation of CNS PPARα and/or PPARγ may contribute to weight gain and obesity. PPARδ is the most abundant isoform in the CNS and is enriched in the hypothalamus, a region of the brain involved in energy homeostasis regulation. Because in peripheral tissues, expression of PPARδ increases lipid oxidative genes and opposes inflammation, we hypothesized that CNS PPARδ protects against the development of DIO. Indeed, genetic neuronal deletion using Nes-Cre loxP technology led to elevated fat mass and decreased lean mass on low-fat diet (LFD), accompanied by leptin resistance and hypothalamic inflammation. Impaired regulation of neuropeptide expression, as well as uncoupling protein 2, and abnormal responses to a metabolic challenge, such as fasting, also occur in the absence of neuronal PPARδ. Consistent with our hypothesis, KO mice gain significantly more fat mass on a high-fat diet (HFD), yet are surprisingly resistant to diet-induced elevations in CNS inflammation and lipid accumulation. We detected evidence of upregulation of PPARγ and target genes of both PPARα and PPARγ, as well as genes of fatty acid oxidation. Thus, our data reveal a previously underappreciated role for neuronal PPARδ in the regulation of body composition, feeding responses, and in the regulation of hypothalamic gene expression.
4 Communities
4 Members
1 Resources
14 MeSH Terms
Effects of dietary composition on energy expenditure during weight-loss maintenance.
Ebbeling CB, Swain JF, Feldman HA, Wong WW, Hachey DL, Garcia-Lago E, Ludwig DS
(2012) JAMA 307: 2627-34
MeSH Terms: Adult, Cholesterol, HDL, Cross-Over Studies, Diet, Carbohydrate-Restricted, Diet, Fat-Restricted, Energy Metabolism, Female, Glycemic Index, Humans, Hydrocortisone, Insulin Resistance, Leptin, Male, Metabolic Syndrome, Nutritive Value, Obesity, Overweight, Plasminogen Activator Inhibitor 1, Triglycerides, Weight Loss, Young Adult
Show Abstract · Added March 20, 2014
CONTEXT - Reduced energy expenditure following weight loss is thought to contribute to weight gain. However, the effect of dietary composition on energy expenditure during weight-loss maintenance has not been studied.
OBJECTIVE - To examine the effects of 3 diets differing widely in macronutrient composition and glycemic load on energy expenditure following weight loss.
DESIGN, SETTING, AND PARTICIPANTS - A controlled 3-way crossover design involving 21 overweight and obese young adults conducted at Children's Hospital Boston and Brigham and Women's Hospital, Boston, Massachusetts, between June 16, 2006, and June 21, 2010, with recruitment by newspaper advertisements and postings.
INTERVENTION - After achieving 10% to 15% weight loss while consuming a run-in diet, participants consumed an isocaloric low-fat diet (60% of energy from carbohydrate, 20% from fat, 20% from protein; high glycemic load), low-glycemic index diet (40% from carbohydrate, 40% from fat, and 20% from protein; moderate glycemic load), and very low-carbohydrate diet (10% from carbohydrate, 60% from fat, and 30% from protein; low glycemic load) in random order, each for 4 weeks.
MAIN OUTCOME MEASURES - Primary outcome was resting energy expenditure (REE), with secondary outcomes of total energy expenditure (TEE), hormone levels, and metabolic syndrome components.
RESULTS - Compared with the pre-weight-loss baseline, the decrease in REE was greatest with the low-fat diet (mean [95% CI], -205 [-265 to -144] kcal/d), intermediate with the low-glycemic index diet (-166 [-227 to -106] kcal/d), and least with the very low-carbohydrate diet (-138 [-198 to -77] kcal/d; overall P = .03; P for trend by glycemic load = .009). The decrease in TEE showed a similar pattern (mean [95% CI], -423 [-606 to -239] kcal/d; -297 [-479 to -115] kcal/d; and -97 [-281 to 86] kcal/d, respectively; overall P = .003; P for trend by glycemic load < .001). Hormone levels and metabolic syndrome components also varied during weight maintenance by diet (leptin, P < .001; 24-hour urinary cortisol, P = .005; indexes of peripheral [P = .02] and hepatic [P = .03] insulin sensitivity; high-density lipoprotein [HDL] cholesterol, P < .001; non-HDL cholesterol, P < .001; triglycerides, P < .001; plasminogen activator inhibitor 1, P for trend = .04; and C-reactive protein, P for trend = .05), but no consistent favorable pattern emerged.
CONCLUSION - Among overweight and obese young adults compared with pre-weight-loss energy expenditure, isocaloric feeding following 10% to 15% weight loss resulted in decreases in REE and TEE that were greatest with the low-fat diet, intermediate with the low-glycemic index diet, and least with the very low-carbohydrate diet.
TRIAL REGISTRATION - clinicaltrials.gov Identifier: NCT00315354.
0 Communities
1 Members
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21 MeSH Terms
Effect of dairy and non-dairy calcium on fecal fat excretion in lactose digester and maldigester obese adults.
Buchowski MS, Aslam M, Dossett C, Dorminy C, Choi L, Acra S
(2010) Int J Obes (Lond) 34: 127-35
MeSH Terms: Adult, Anthropometry, Calcium, Dietary, Cross-Over Studies, Dairy Products, Diet, Fat-Restricted, Energy Intake, Energy Metabolism, Feces, Female, Humans, Lactose, Lactose Intolerance, Male, Middle Aged, Obesity, Weight Loss, Young Adult
Show Abstract · Added December 10, 2013
BACKGROUND - The effect of dietary calcium (Ca) on fecal fat excretion in lactose maldigestion is not known.
OBJECTIVE - To investigate the effect of dairy and non-dairy dietary Ca on fecal fat excretion in lactose digesters and maldigesters during moderate energy restriction.
DESIGN - A randomized cross-over trial comparing the effect of 500 mg versus 1500 mg dairy and non-dairy Ca on fecal fat excretion in 34 healthy adults during moderate (-30%) energy restriction induced weight loss for 12 weeks. The participants were classified as lactose digester or maldigester on the basis of breath hydrogen test.
MEASUREMENTS - Anthropometric parameters and body composition, resting energy expenditure, energy and nutrient intake, fecal fat, physical activity, blood pressure, blood and urine sampling for pertinent measurements.
RESULTS - Fecal fat loss expressed as percent of fat intake was significantly higher with 1500 mg (high Ca) as compared with 500 mg (low Ca) Ca intake per day (mean: 3.0%; 95% CI: 2.3 to 3.7%; P<0.001) independent of Ca source and lactose digestion status.
CONCLUSIONS - During a moderate energy restriction induced weight loss, a high-Ca diet causes an increase in fecal fat excretion independent of Ca source. Ca intake related fecal fat loss is also independent of the ability to digest lactose and it is not diminished over time (US Clinical Trial Registration: Clinicaltrials.gov NCT00808275).
1 Communities
2 Members
0 Resources
18 MeSH Terms
Metabolic implications of reduced heart-type fatty acid binding protein in insulin resistant cardiac muscle.
Shearer J, Fueger PT, Wang Z, Bracy DP, Wasserman DH, Rottman JN
(2008) Biochim Biophys Acta 1782: 586-92
MeSH Terms: Animals, Blood Glucose, Blood Pressure, Body Weight, Diet, Fat-Restricted, Dietary Fats, Fatty Acid Binding Protein 3, Fatty Acid-Binding Proteins, Fatty Acids, Nonesterified, Female, Glucose Clamp Technique, Heart, Insulin, Insulin Resistance, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Inbred Strains, Mice, Transgenic, Myocardium, Ventricular Function
Show Abstract · Added December 22, 2010
Insulin resistance is characterized by elevated rates of cardiac fatty acid utilization resulting in reduced efficiency and cardiomyopathy. One potential therapeutic approach is to limit the uptake and oxidation of fatty acids. The aims of this study were to determine whether a quantitative reduction in heart-type fatty acid binding protein (FABP3) normalizes cardiac substrate utilization without altering cardiac function. Transgenic (FABP3(+/-)) and wild-type (WT) littermates were studied following low fat (LF) or high fat (HF) diets, with HF resulting in obese, insulin-resistant mice. Cardiovascular function (systolic blood pressure, % fractional shortening) and heart dimension were measured at weaning and every month afterward for 3 mo. During this period cardiovascular function was the same independent of genotype and diet. Catheters were surgically implanted in the carotid artery and jugular vein for sampling and infusions in mice at 4 mo of age. Following 5 d recovery, mice underwent either a saline infusion or a hyperinsulinemic-euglycemic clamp (4 mU kg(-1) min(-1)). Indices of long chain fatty acid and glucose utilization (R(f), R(g); mumol g wet weight(-1) min(-1)) were obtained using 2-deoxy[(3)H]glucose and [(125)I]-15-rho-iodophenyl)-3-R,S-methylpentadecanoic acid. FABP3(+/-) had enhanced cardiac R(g) compared with WT during saline infusion in both LF and HF. FABP3(+/-) abrogated the HF-induced decrement in insulin-stimulated cardiac R(g). On a HF diet, FABP(+/-) but not WT had an increased reliance on fatty acids (R(f)) during insulin stimulation. In conclusion, cardiac insulin resistance and glucose uptake is largely corrected by a reduction in FABP3 in vivo without contemporaneous deleterious effects on cardiac function.
1 Communities
2 Members
0 Resources
22 MeSH Terms
Combined adipocyte-macrophage fatty acid-binding protein deficiency improves metabolism, atherosclerosis, and survival in apolipoprotein E-deficient mice.
Boord JB, Maeda K, Makowski L, Babaev VR, Fazio S, Linton MF, Hotamisligil GS
(2004) Circulation 110: 1492-8
MeSH Terms: Adipose Tissue, Animals, Aorta, Apolipoproteins E, Arteriosclerosis, Blood Glucose, Carrier Proteins, Cholesterol, Diet, Fat-Restricted, Fatty Acid-Binding Proteins, Female, Hyperlipoproteinemia Type II, Insulin Resistance, Longevity, Male, Mesentery, Metabolic Syndrome, Mice, Mice, Inbred C57BL, Mice, Knockout, Obesity, Organ Size, Triglycerides
Show Abstract · Added May 27, 2014
BACKGROUND - The adipocyte fatty acid-binding protein (FABP) aP2 is expressed by adipocytes and macrophages and modulates insulin resistance, glucose and lipid metabolism, and atherosclerosis. Insulin sensitivity is improved in obese but not in lean aP2-deficient mice. A second fatty acid-binding protein, mal1, also is expressed in adipocytes and macrophages, and mal1 deficiency produces similar effects on insulin resistance. We tested the hypothesis that combined aP2 and mal1 deficiency would produce synergistic effects on metabolism and reduce atherosclerosis in apolipoprotein E-deficient (apoE-/-) mice.
METHODS AND RESULTS - Male and female apoE-/- mice null for both aP2 and mal1 (3KO) and apoE-/- controls were fed a low-fat chow diet for 16 or 56 weeks. Lean 3KO mice had significantly lower serum cholesterol and triglycerides as well as improved insulin and glucose tolerance as compared with controls. Analysis of atherosclerotic lesions in the 3KO mice showed dramatic reductions in both early (20 weeks) and late-stage (60 weeks) atherosclerosis. Strikingly, survival in the 3KO mice was improved by 67% as compared with apoE-/- controls when challenged with the Western diet for 1 year.
CONCLUSIONS - Combined aP2 and mal1 deficiency improved glucose and lipid metabolism, reduced atherosclerosis, and improved survival in apoE-/- mice, making these proteins important therapeutic targets for the prevention of the cardiovascular consequences of the metabolic syndrome.
0 Communities
1 Members
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23 MeSH Terms