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Gabapentin inhibits catecholamine release from adrenal chromaffin cells.
Todd RD, McDavid SM, Brindley RL, Jewell ML, Currie KP
(2012) Anesthesiology 116: 1013-24
MeSH Terms: Adrenal Glands, Amines, Animals, Calcium, Calcium Channels, Calcium Signaling, Catecholamines, Cattle, Cholinergic Agonists, Chromaffin Cells, Cyclohexanecarboxylic Acids, Dose-Response Relationship, Drug, Excitatory Amino Acid Antagonists, Gabapentin, Hemodynamics, In Vitro Techniques, Patch-Clamp Techniques, Potassium Chloride, Secretory Vesicles, gamma-Aminobutyric Acid
Show Abstract · Added March 30, 2013
BACKGROUND - Gabapentin is most commonly prescribed for chronic pain, but acute perioperative effects, including preemptive analgesia and hemodynamic stabilization, have been reported. Adrenal chromaffin cells are a widely used model to investigate neurosecretion, and adrenal catecholamines play important physiologic roles and contribute to the acute stress response. However, the effects of gabapentin on adrenal catecholamine release have never been tested.
METHODS - Primary cultures of bovine adrenal chromaffin cells were treated with gabapentin or vehicle for 18-24 h. The authors quantified catecholamine secretion from dishes of cells using high-performance liquid chromatography and resolved exocytosis of individual secretory vesicles from single cells using carbon fiber amperometry. Voltage-gated calcium channel currents were recorded using patch clamp electrophysiology and intracellular [Ca2+] using fluorescent imaging.
RESULTS - Gabapentin produced statistically significant reductions in catecholamine secretion evoked by cholinergic agonists (24 ± 3%, n = 12) or KCl (16 ± 4%, n = 8) (mean ± SEM) but did not inhibit Ca2+ entry or calcium channel currents. Amperometry (n = 51 cells) revealed that gabapentin inhibited the number of vesicles released upon stimulation, with no change in quantal size or kinetics of these unitary events.
CONCLUSIONS - The authors show Ca2+ entry was not inhibited by gabapentin but was less effective at triggering vesicle fusion. The work also demonstrates that chromaffin cells are a useful model for additional investigation of the cellular mechanism(s) by which gabapentin controls neurosecretion. In addition, it identifies altered adrenal catecholamine release as a potential contributor to some of the beneficial perioperative effects of gabapentin.
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20 MeSH Terms
Regulation and function of COX-2 gene expression in isolated gastric parietal cells.
Pausawasdi N, Ramamoorthy S, Crofford LJ, Askari FK, Todisco A
(2002) Am J Physiol Gastrointest Liver Physiol 282: G1069-78
MeSH Terms: Animals, Carbachol, Cells, Cultured, Cholinergic Agonists, Cyclooxygenase 2, Cyclooxygenase 2 Inhibitors, Cyclooxygenase Inhibitors, Dogs, Enzyme Inhibitors, Gene Expression Regulation, Enzymologic, Histamine, Imidazoles, Indoles, Isoenzymes, Maleimides, NF-kappa B, Nitrobenzenes, Parietal Cells, Gastric, Prostaglandin-Endoperoxide Synthases, Prostaglandins, Pyridines, Sulfonamides
Show Abstract · Added September 18, 2013
We examined expression, function, and regulation of the cyclooxygenase (COX)-2 gene in gastric parietal cells. COX-2-specific mRNA was isolated from purified (>95%) canine gastric parietal cells in primary culture and measured by Northern blots using a human COX-2 cDNA probe. Carbachol was the most potent inducer of COX-2 gene expression. Gastrin and histamine exhibited minor stimulatory effects. Carbachol-stimulated expression was inhibited by intracellular Ca(2+) chelator 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid-AM (90%), protein kinase C (PKC) inhibitor GF-109203X (48%), and p38 kinase inhibitor SB-203580 (48%). Nuclear factor (NF)-kappaB inhibitor 1-pyrrolidinecarbodithioic acid inhibited carbachol-stimulated expression by 80%. Similar results were observed in the presence of adenoviral vector Ad.dom.neg.IkappaB, which expresses a repressor of NF-kappaB. Addition of SB-203580 with Ad.dom.neg.IkappaB almost completely blocked carbachol stimulation of COX-2 gene expression. We examined the effect of carbachol on PGE(2) release by enzyme-linked immunoassay. Carbachol induced PGE(2) release. Ad.dom.neg.IkappaB, alone or with SB-203580, produced, respectively, partial (70%) and almost complete (>80%) inhibition of carbachol-stimulated PGE(2) production. Selective COX-2 inhibitor NS-398 blocked carbachol-stimulated PGE(2) release without affecting basal PGE(2) production. In contrast, indomethacin inhibited both basal and carbachol-stimulated PGE(2) release. Carbachol induces COX-2 gene expression in the parietal cells through signaling pathways that involve intracellular Ca(2+), PKC, p38 kinase, and activation of NF-kappaB. The functional significance of these effects seems to be stimulation of PGE(2) release.
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22 MeSH Terms