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Publication Record


Electron Microscopy of Living Cells During in Situ Fluorescence Microscopy.
Liv N, van Oosten Slingeland DS, Baudoin JP, Kruit P, Piston DW, Hoogenboom JP
(2016) ACS Nano 10: 265-73
MeSH Terms: Animals, Bioreactors, Cell Line, Cells, Immobilized, Chlorocebus aethiops, Endocytosis, Fibroblasts, Microscopy, Electron, Scanning, Optical Imaging, Quantum Dots
Show Abstract · Added February 4, 2016
We present an approach toward dynamic nanoimaging: live fluorescence of cells encapsulated in a bionanoreactor is complemented with in situ scanning electron microscopy (SEM) on an integrated microscope. This allows us to take SEM snapshots on-demand, that is, at a specific location in time, at a desired region of interest, guided by the dynamic fluorescence imaging. We show that this approach enables direct visualization, with EM resolution, of the distribution of bioconjugated quantum dots on cellular extensions during uptake and internalization.
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