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Results: 1 to 10 of 13

Publication Record


Comparative genomics of biotechnologically important yeasts.
Riley R, Haridas S, Wolfe KH, Lopes MR, Hittinger CT, Göker M, Salamov AA, Wisecaver JH, Long TM, Calvey CH, Aerts AL, Barry KW, Choi C, Clum A, Coughlan AY, Deshpande S, Douglass AP, Hanson SJ, Klenk HP, LaButti KM, Lapidus A, Lindquist EA, Lipzen AM, Meier-Kolthoff JP, Ohm RA, Otillar RP, Pangilinan JL, Peng Y, Rokas A, Rosa CA, Scheuner C, Sibirny AA, Slot JC, Stielow JB, Sun H, Kurtzman CP, Blackwell M, Grigoriev IV, Jeffries TW
(2016) Proc Natl Acad Sci U S A 113: 9882-7
MeSH Terms: Ascomycota, Biotechnology, Evolution, Molecular, Fungal Proteins, Genetic Code, Genome, Fungal, Genomics, Metabolic Networks and Pathways, Phylogeny, Species Specificity, Yeasts
Show Abstract · Added April 6, 2017
Ascomycete yeasts are metabolically diverse, with great potential for biotechnology. Here, we report the comparative genome analysis of 29 taxonomically and biotechnologically important yeasts, including 16 newly sequenced. We identify a genetic code change, CUG-Ala, in Pachysolen tannophilus in the clade sister to the known CUG-Ser clade. Our well-resolved yeast phylogeny shows that some traits, such as methylotrophy, are restricted to single clades, whereas others, such as l-rhamnose utilization, have patchy phylogenetic distributions. Gene clusters, with variable organization and distribution, encode many pathways of interest. Genomics can predict some biochemical traits precisely, but the genomic basis of others, such as xylose utilization, remains unresolved. Our data also provide insight into early evolution of ascomycetes. We document the loss of H3K9me2/3 heterochromatin, the origin of ascomycete mating-type switching, and panascomycete synteny at the MAT locus. These data and analyses will facilitate the engineering of efficient biosynthetic and degradative pathways and gateways for genomic manipulation.
0 Communities
1 Members
0 Resources
11 MeSH Terms
Use of Human Hybridoma Technology To Isolate Human Monoclonal Antibodies.
Smith SA, Crowe JE
(2015) Microbiol Spectr 3: AID-0027-2014
MeSH Terms: Antibodies, Monoclonal, Antigens, B-Lymphocytes, Biotechnology, Cell Fusion, Humans, Hybridomas
Show Abstract · Added January 26, 2016
The human hybridoma technique offers an important approach for isolation of human monoclonal antibodies. A diversity of approaches can be used with varying success. Recent technical advances in expanding the starting number of human antigen-specific B cells, improving fusion efficiency, and isolating new myeloma partners and new cell cloning methods have enabled the development of protocols that make the isolation of human monoclonal antibodies from blood samples feasible. Undoubtedly, additional innovations that could improve efficiency are possible.
0 Communities
1 Members
0 Resources
7 MeSH Terms
Editorial overview: pharmaceutical biotechnology: engineering cells for high quality biopharmaceuticals production.
Junker BH, Young JD
(2014) Curr Opin Biotechnol 30: viii-x
MeSH Terms: Animals, Biopharmaceutics, Biotechnology, CHO Cells, Cricetulus, Glycosylation
Added January 23, 2015
0 Communities
1 Members
0 Resources
6 MeSH Terms
Breaking the code of DNA binding specificity of TAL-type III effectors.
Boch J, Scholze H, Schornack S, Landgraf A, Hahn S, Kay S, Lahaye T, Nickstadt A, Bonas U
(2009) Science 326: 1509-12
MeSH Terms: Amino Acid Motifs, Amino Acid Sequence, Arabidopsis, Bacterial Proteins, Base Pairing, Base Sequence, Biotechnology, Capsicum, DNA, Plant, DNA-Binding Proteins, Genes, Plant, Models, Biological, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Repetitive Sequences, Amino Acid, Tobacco, Transcription Factors, Transcriptional Activation, Xanthomonas
Show Abstract · Added June 6, 2012
The pathogenicity of many bacteria depends on the injection of effector proteins via type III secretion into eukaryotic cells in order to manipulate cellular processes. TAL (transcription activator-like) effectors from plant pathogenic Xanthomonas are important virulence factors that act as transcriptional activators in the plant cell nucleus, where they directly bind to DNA via a central domain of tandem repeats. Here, we show how target DNA specificity of TAL effectors is encoded. Two hypervariable amino acid residues in each repeat recognize one base pair in the target DNA. Recognition sequences of TAL effectors were predicted and experimentally confirmed. The modular protein architecture enabled the construction of artificial effectors with new specificities. Our study describes the functionality of a distinct type of DNA binding domain and allows the design of DNA binding domains for biotechnology.
1 Communities
0 Members
0 Resources
20 MeSH Terms
Frontiers of Biomedical Imaging Science 2009: workshop report and research opportunities.
Yankeelov TE, Avison MJ, Damon BM, Manning HC, Peterson TE, Gore JC
(2009) Cancer Res 69: 7902-4
MeSH Terms: Animals, Biotechnology, Diabetes Mellitus, Diagnostic Imaging, Disease Models, Animal, Humans, Mental Disorders, Neoplasms
Added December 10, 2013
0 Communities
6 Members
0 Resources
8 MeSH Terms
Development of a tissue engineered heart valve for pediatrics: a case study in bioengineering ethics.
Merryman WD
(2008) Sci Eng Ethics 14: 93-101
MeSH Terms: Biotechnology, Child, Preschool, Decision Making, Equipment Failure, Heart Valve Prosthesis, Humans, Pediatrics
Show Abstract · Added February 12, 2015
The following hypothetical case study was developed for bioengineering students and is concerned with choosing between two devices used for development of a pediatric tissue engineered heart valve (TEHV). This case is intended to elicit assessment of the devices, possible future outcomes, and ramifications of the decision making. It is framed in light of two predominant ethical theories: utilitarianism and rights of persons. After the case was presented to bioengineering graduate students, they voted on which device should be released. The results revealed that these bioengineering students preferred the more reliable (and substantially more expensive) design, though this choice precludes the majority of the world from having access to this technology. This case is intended to examine and explore where the balance lies between design, cost, and adequate distribution of biomedical devices.
0 Communities
1 Members
0 Resources
7 MeSH Terms
[RNA interference: biology and perspectives of application in biomedicine and biotechnology].
Vil'gel'm AE, Chumakov SP, Prasolov VS
(2006) Mol Biol (Mosk) 40: 387-403
MeSH Terms: Animals, Biotechnology, Humans, Neoplasms, RNA Interference, RNA, Small Interfering, Retroelements, Virus Diseases
Show Abstract · Added June 14, 2013
RNA interference (RNAi) is among the most particular mechanisms of gene expression regulation. Besides, small interfering RNAs are significant players in cell defence either from viral infection or retrotransposons. Medical utilization of RNAi gives a handful of ways to cure viral and oncological illnesses. RNA interference, also, represents a useful tool for research, because it allows quick production of monogene functional knockouts. In this review we describe the most recent conceptions about RNAi mechanisms and actual approaches for it's usage.
0 Communities
1 Members
0 Resources
8 MeSH Terms
Exploiting the versatility of human cytochrome P450 enzymes: the promise of blue roses from biotechnology.
Gillam EM, Guengerich FP
(2001) IUBMB Life 52: 271-7
MeSH Terms: Animals, Aryl Hydrocarbon Hydroxylases, Biotechnology, Cytochrome P-450 CYP2A6, Cytochrome P-450 Enzyme System, Escherichia coli, Humans, Indigo Carmine, Indoles, Mixed Function Oxygenases, Models, Chemical, Plants, Genetically Modified, Recombinant Proteins
Show Abstract · Added May 26, 2014
The cytochrome P450 (P450) enzymes involved in drug metabolism are among the most versatile biological catalysts known. A small number of discrete forms of human P450 are capable of catalyzing the monooxygenation of a practically unlimited variety of xenobiotic substrates, with each enzyme showing a more or less wide and overlapping substrate range. This versatility makes P450s ideally suited as starting materials for engineering designer catalysts for industrial applications. In the course of heterologous expression of P450s in bacteria, we observed the unexpected formation of blue pigments. Although this was initially assumed to be an artifact, subsequent work led to the discovery of a new function of P450s in intermediary metabolism and toxicology, new screens for protein engineering, and potential applications in the dye and horticulture industries.
0 Communities
1 Members
0 Resources
13 MeSH Terms
Bar-coding biomolecules with fluorescent nanocrystals.
Rosenthal SJ
(2001) Nat Biotechnol 19: 621-2
MeSH Terms: Biotechnology, Cadmium Compounds, Crystallization, Fluorescent Dyes, Microspheres, Nucleic Acid Hybridization, Oligonucleotide Array Sequence Analysis, Selenium Compounds, Semiconductors, Spectrometry, Fluorescence
Added May 27, 2014
0 Communities
1 Members
0 Resources
10 MeSH Terms
Two-color GFP expression system for C. elegans.
Miller DM, Desai NS, Hardin DC, Piston DW, Patterson GH, Fleenor J, Xu S, Fire A
(1999) Biotechniques 26: 914-8, 920-1
MeSH Terms: Amino Acid Substitution, Animals, Animals, Genetically Modified, Biotechnology, Caenorhabditis elegans, Evaluation Studies as Topic, Gene Expression, Green Fluorescent Proteins, Luminescent Proteins, Muscles, Plasmids, Spectrometry, Fluorescence
Show Abstract · Added February 3, 2014
We describe the use of modified versions of the Aequora victoria green fluorescent protein (GFP) to simultaneously follow the expression and distribution of two different proteins in the nematode, Caenorhabditis elegans. A cyan-colored GFP derivative, designated CFP, contains amino acid (aa) substitutions Y66W, N146I, M153T and V163A relative to the original GFP sequence and is similar to the previously reported "W7" form. A yellow-shifted GFP derivative, designated YFP, contains aa substitutions S65G, V68A, S72A and T203Y and is similar to the previously described "I0C" variant. Coding regions for CFP and YFP were constructed in the context of a high-activity C. elegans expression system. Previously characterized promoters and localization signals have been used to express CFP and YFP in C. elegans. Filter sets designed to distinguish YFP and CFP fluorescence spectra allowed visualization of the two distinct forms of GFP in neurons and in muscle cells. A series of expression vectors carrying CFP and YFP have been constructed and are being made available to the scientific community.
0 Communities
1 Members
0 Resources
12 MeSH Terms