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Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model.
Bichell TJV, Wegrzynowicz M, Tipps KG, Bradley EM, Uhouse MA, Bryan M, Horning K, Fisher N, Dudek K, Halbesma T, Umashanker P, Stubbs AD, Holt HK, Kwakye GF, Tidball AM, Colbran RJ, Aschner M, Neely MD, Di Pardo A, Maglione V, Osmand A, Bowman AB
(2017) Biochim Biophys Acta Mol Basis Dis 1863: 1596-1604
MeSH Terms: Animals, Arginase, Corpus Striatum, Disease Models, Animal, Huntington Disease, Male, Manganese, Mice, Neurons, Urea
Show Abstract · Added April 26, 2017
Huntington's disease (HD) is caused by a mutation in the huntingtin gene (HTT), resulting in profound striatal neurodegeneration through an unknown mechanism. Perturbations in the urea cycle have been reported in HD models and in HD patient blood and brain. In neurons, arginase is a central urea cycle enzyme, and the metal manganese (Mn) is an essential cofactor. Deficient biological responses to Mn, and reduced Mn accumulation have been observed in HD striatal mouse and cell models. Here we report in vivo and ex vivo evidence of a urea cycle metabolic phenotype in a prodromal HD mouse model. Further, either in vivo or in vitro Mn supplementation reverses the urea-cycle pathology by restoring arginase activity. We show that Arginase 2 (ARG2) is the arginase enzyme present in these mouse brain models, with ARG2 protein levels directly increased by Mn exposure. ARG2 protein is not reduced in the prodromal stage, though enzyme activity is reduced, indicating that altered Mn bioavailability as a cofactor leads to the deficient enzymatic activity. These data support a hypothesis that mutant HTT leads to a selective deficiency of neuronal Mn at an early disease stage, contributing to HD striatal urea-cycle pathophysiology through an effect on arginase activity.
Copyright © 2017 The Author(s). Published by Elsevier B.V. All rights reserved.
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10 MeSH Terms
The myeloid immune signature of enterotoxigenic Bacteroides fragilis-induced murine colon tumorigenesis.
Thiele Orberg E, Fan H, Tam AJ, Dejea CM, Destefano Shields CE, Wu S, Chung L, Finard BB, Wu X, Fathi P, Ganguly S, Fu J, Pardoll DM, Sears CL, Housseau F
(2017) Mucosal Immunol 10: 421-433
MeSH Terms: Animals, Arginase, Bacterial Toxins, Bacteroides Infections, Bacteroides fragilis, Carcinogenesis, Cell Differentiation, Cell Proliferation, Cells, Cultured, Colon, Colorectal Neoplasms, Disease Models, Animal, Epithelial Cells, Genes, APC, Humans, Immune Tolerance, Interleukin-17, Metalloendopeptidases, Mice, Mice, Inbred C57BL, Mice, Mutant Strains, Myeloid-Derived Suppressor Cells, Nitric Oxide Synthase Type II, T-Lymphocytes, Transcriptome
Show Abstract · Added March 20, 2018
Enterotoxigenic Bacteroides fragilis (ETBF), a human commensal and candidate pathogen in colorectal cancer (CRC), is a potent initiator of interleukin-17 (IL-17)-dependent colon tumorigenesis in Min mice. We examined the role of IL-17 and ETBF on the differentiation of myeloid cells into myeloid-derived suppressor cells (MDSCs) and tumor-associated macrophages, which are known to promote tumorigenesis. The myeloid compartment associated with ETBF-induced colon tumorigenesis in Min mice was defined using flow cytometry and gene expression profiling. Cell-sorted immature myeloid cells were functionally assayed for inhibition of T-cell proliferation and inducible nitric oxide synthase expression to delineate MDSC populations. A comparison of ETBF infection with that of other oncogenic bacteria (Fusobacterium nucleatum or pksEscherichia coli) revealed a specific, ETBF-associated colonic immune infiltrate. ETBF-triggered colon tumorigenesis is associated with an IL-17-driven myeloid signature characterized by subversion of steady-state myelopoiesis in favor of the generation of protumoral monocytic-MDSCs (MO-MDSCs). Combined action of the B. fragilis enterotoxin BFT and IL-17 on colonic epithelial cells promoted the differentiation of MO-MDSCs, which selectively upregulated Arg1 and Nos2, produced NO, and suppressed T-cell proliferation. Evidence of a pathogenic inflammatory signature in humans colonized with ETBF may allow for the identification of populations at risk for developing colon cancer.
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25 MeSH Terms
L-Arginine Availability and Metabolism Is Altered in Ulcerative Colitis.
Coburn LA, Horst SN, Allaman MM, Brown CT, Williams CS, Hodges ME, Druce JP, Beaulieu DB, Schwartz DA, Wilson KT
(2016) Inflamm Bowel Dis 22: 1847-58
MeSH Terms: Amino Acid Transport Systems, Basic, Amino Acids, Arginase, Arginine, Biological Availability, Case-Control Studies, Citrulline, Clinical Trials as Topic, Colitis, Ulcerative, Colon, Crohn Disease, Diet Records, Humans, Nitric Oxide Synthase Type II, Prospective Studies, RNA, Messenger, Severity of Illness Index
Show Abstract · Added April 24, 2016
BACKGROUND - L-arginine (L-Arg) is the substrate for both inducible nitric oxide (NO) synthase (NOS2) and arginase (ARG) enzymes. L-Arg is actively transported into cells by means of cationic amino acid transporter (SLC7) proteins. We have linked L-Arg and arginase 1 activity to epithelial restitution. Our aim was to determine if L-Arg, related amino acids, and metabolic enzymes are altered in ulcerative colitis (UC).
METHODS - Serum and colonic tissues were prospectively collected from 38 control subjects and 137 UC patients. Dietary intake, histologic injury, and clinical disease activity were assessed. Amino acid levels were measured by high-performance liquid chromatography. Messenger RNA (mRNA) levels were measured by real-time PCR. Colon tissue samples from 12 Crohn's disease patients were obtained for comparison.
RESULTS - Dietary intake of arginine and serum L-Arg levels were not different in UC patients versus control subjects. In active UC, tissue L-Arg was decreased, whereas L-citrulline (L-Cit) and the L-Cit/L-Arg ratio were increased. This pattern was also seen when paired involved (left) versus uninvolved (right) colon tissues in UC were assessed. In active UC, SLC7A2 and ARG1 mRNA levels were decreased, whereas ARG2 and NOS2 were increased. Similar alterations in mRNA expression occurred in tissues from Crohn's disease patients. In involved UC, SLC7A2 and ARG1 mRNA levels were decreased, and NOS2 and ARG2 increased, when compared with uninvolved tissues.
CONCLUSIONS - Patients with UC exhibit diminished tissue L-Arg, likely attributable to decreased cellular uptake and increased consumption by NOS2. These findings combined with decreased ARG1 expression indicate a pattern of dysregulated L-Arg availability and metabolism in UC.
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17 MeSH Terms
Arginase 2 deletion leads to enhanced M1 macrophage activation and upregulated polyamine metabolism in response to Helicobacter pylori infection.
Hardbower DM, Asim M, Murray-Stewart T, Casero RA, Verriere T, Lewis ND, Chaturvedi R, Piazuelo MB, Wilson KT
(2016) Amino Acids 48: 2375-88
MeSH Terms: Animals, Arginase, Biogenic Polyamines, Helicobacter Infections, Helicobacter pylori, Immune Evasion, Macrophage Activation, Macrophages, Mice, Mice, Knockout, Nitric Oxide Synthase Type II, Stomach, Th1 Cells, Th17 Cells
Show Abstract · Added April 22, 2016
We reported that arginase 2 (ARG2) deletion results in increased gastritis and decreased bacterial burden during Helicobacter pylori infection in mice. Our studies implicated a potential role for inducible nitric oxide (NO) synthase (NOS2), as Arg2 (-/-) mice exhibited increased NOS2 levels in gastric macrophages, and NO can kill H. pylori. We now bred Arg2 (-/-) to Nos2 (-/-) mice, and infected them with H. pylori. Compared to wild-type mice, both Arg2 (-/-) and Arg2 (-/-) ;Nos2 (-/-) mice exhibited increased gastritis and decreased colonization, the latter indicating that the effect of ARG2 deletion on bacterial burden was not mediated by NO. While Arg2 (-/-) mice demonstrated enhanced M1 macrophage activation, Nos2 (-/-) and Arg2 (-/-) ;Nos2 (-/-) mice did not demonstrate these changes, but exhibited increased CXCL1 and CXCL2 responses. There was an increased expression of the Th1/Th17 cytokines, interferon gamma and interleukin 17, in gastric tissues and splenic T-cells from Arg2 (-/-), but not Nos2 (-/-) or Arg2 (-/-) ;Nos2 (-/-) mice. Gastric tissues from infected Arg2 (-/-) mice demonstrated increased expression of arginase 1, ornithine decarboxylase, adenosylmethionine decarboxylase 1, spermidine/spermine N (1)-acetyltransferase 1, and spermine oxidase, along with increased spermine levels. These data indicate that ARG2 deletion results in compensatory upregulation of gastric polyamine synthesis and catabolism during H. pylori infection, which may contribute to increased gastric inflammation and associated decreased bacterial load. Overall, the finding of this study is that ARG2 contributes to the immune evasion of H. pylori by restricting M1 macrophage activation and polyamine metabolism.
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14 MeSH Terms
Dual role of arginine metabolism in establishing pathogenesis.
Gogoi M, Datey A, Wilson KT, Chakravortty D
(2016) Curr Opin Microbiol 29: 43-8
MeSH Terms: Animals, Arginase, Arginine, Bacteria, Bacterial Infections, Energy Metabolism, Host-Pathogen Interactions, Humans, Immune Evasion, Mice, Nitric Oxide, Nitric Oxide Synthase Type II
Show Abstract · Added February 15, 2016
Arginine is an integral part of host defense when invading pathogens are encountered. The arginine metabolite nitric oxide (NO) confers antimicrobial properties, whereas the metabolite ornithine is utilized for polyamine synthesis. Polyamines are crucial to tissue repair and anti-inflammatory responses. iNOS/arginase balance can determine Th1/Th2 response. Furthermore, the host arginine pool and its metabolites are utilized as energy sources by various pathogens. Apart from its role as an immune modulator, recent studies have also highlighted the therapeutic effects of arginine. This article sheds light upon the roles of arginine metabolism during pathological conditions and its therapeutic potential.
Copyright © 2015 Elsevier Ltd. All rights reserved.
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12 MeSH Terms
Manganese Is Essential for Neuronal Health.
Horning KJ, Caito SW, Tipps KG, Bowman AB, Aschner M
(2015) Annu Rev Nutr 35: 71-108
MeSH Terms: Arginase, Bile, Blood-Brain Barrier, Brain, Enzyme Activation, Glutamate-Ammonia Ligase, Health Status, Homeostasis, Humans, Huntington Disease, Intestinal Absorption, Manganese, Nervous System Diseases, Neurons, Parkinson Disease
Show Abstract · Added February 15, 2016
The understanding of manganese (Mn) biology, in particular its cellular regulation and role in neurological disease, is an area of expanding interest. Mn is an essential micronutrient that is required for the activity of a diverse set of enzymatic proteins (e.g., arginase and glutamine synthase). Although necessary for life, Mn is toxic in excess. Thus, maintaining appropriate levels of intracellular Mn is critical. Unlike other essential metals, cell-level homeostatic mechanisms of Mn have not been identified. In this review, we discuss common forms of Mn exposure, absorption, and transport via regulated uptake/exchange at the gut and blood-brain barrier and via biliary excretion. We present the current understanding of cellular uptake and efflux as well as subcellular storage and transport of Mn. In addition, we highlight the Mn-dependent and Mn-responsive pathways implicated in the growing evidence of its role in Parkinson's disease and Huntington's disease. We conclude with suggestions for future focuses of Mn health-related research.
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15 MeSH Terms
Editorial: Orchestration of macrophage polarization by polyamines.
Gobert AP, Wilson KT
(2012) J Leukoc Biol 91: 677-9
MeSH Terms: Animals, Arginase, Cytokines, Inflammation Mediators, Interleukin-4, Lipopolysaccharides, Macrophages, Polyamines
Added March 5, 2014
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8 MeSH Terms
Arginase II inhibition prevents nitrate tolerance.
Khong SM, Andrews KL, Huynh NN, Venardos K, Aprico A, Michell DL, Zarei M, Moe KT, Dusting GJ, Kaye DM, Chin-Dusting JP
(2012) Br J Pharmacol 166: 2015-23
MeSH Terms: Animals, Aorta, Thoracic, Arginase, Arginine, Boronic Acids, Drug Tolerance, Human Umbilical Vein Endothelial Cells, Humans, In Vitro Techniques, Mice, Mice, Inbred C57BL, Mice, Knockout, Nitric Oxide Synthase Type III, Nitroglycerin, Reactive Oxygen Species, Vasodilator Agents
Show Abstract · Added July 21, 2014
BACKGROUND AND PURPOSE - Nitrate tolerance, the loss of vascular responsiveness with continued use of nitrates, remains incompletely understood and is a limitation of these therapeutic agents. Vascular superoxide, generated by uncoupled endothelial NOS (eNOS), may play a role. As arginase competes with eNOS for L-arginine and may exacerbate the production of reactive oxygen species (ROS), we hypothesized that arginase inhibition might reduce nitrate tolerance.
EXPERIMENTAL APPROACH - Vasodilator responses were measured in aorta from C57Bl/6 and arginase II knockout (argII -/-) mice using myography. Uncoupling of eNOS, determined as eNOS monomer : dimer ratio, was assessed using low-temperature SDS-PAGE and ROS levels were measured using L-012 and lucigenin-enhanced chemiluminescence.
KEY RESULTS - Repeated application of glyceryl trinitrate (GTN) on aorta isolated from C57Bl/6 mice produced a 32-fold rightward shift of the concentration-response curve. However this rightward shift (or resultant tolerance) was not observed in the presence of the arginase inhibitor (s)-(2-boronethyl)-L-cysteine HCl (BEC; 100 µM) nor in aorta isolated from argII -/- mice. Similar findings were obtained after inducing nitrate tolerance in vivo. Repeated administration of GTN in human umbilical vein endothelial cells induced uncoupling of eNOS from its dimeric state and increased ROS levels, which were reduced with arginase inhibition and exogenous L-arginine. Aortae from GTN tolerant C57Bl/6 mice exhibited increased arginase activity and ROS production, whereas vessels from argII -/- mice did not.
CONCLUSION AND IMPLICATIONS - Arginase II removal prevents nitrate tolerance. This may be due to decreased uncoupling of eNOS and consequent ROS production.
© 2012 The Authors. British Journal of Pharmacology © 2012 The British Pharmacological Society.
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16 MeSH Terms
Endothelial dysfunction in hypertension: the role of arginase.
Michell DL, Andrews KL, Chin-Dusting JP
(2011) Front Biosci (Schol Ed) 3: 946-60
MeSH Terms: Arginase, Endothelium, Vascular, Humans, Hypertension, Nitric Oxide, Reactive Oxygen Species, Signal Transduction
Show Abstract · Added July 21, 2014
Essential hypertension is the leading risk factor for mortality worldwide, accountable for 13% of deaths globally. Despite numerous therapies available uncontrolled hypertension is still very prevalent today and a large subset are shown to have treatment resistant hypertension. Several cardiovascular diseases including hypertension result in endothelial dysfunction and inflammation. Once thought of as a passive barrier between blood flow and tissue the endothelium is now considered a main hub for maintaining vascular tone, structure and haemostasis. Several pathways occur in the endothelium that can result in dysfunction and altered vascular stasis. Such pathways include the impairment of the vasodilator nitric oxide (NO), increases in pro-inflammatory pathways such as ROS (reactive oxygen species) production and also recent reports suggest that the enzyme arginase, associated with the L-arginine-urea cycle, may be an important factor that is increased in hypertension. These pathways may offer alternative mechanisms to treat the complications associated with hypertension rather than the conventional therapies that aim to lower blood pressure.
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7 MeSH Terms
Methods to evaluate alterations in polyamine metabolism caused by Helicobacter pylori infection.
Gobert AP, Chaturvedi R, Wilson KT
(2011) Methods Mol Biol 720: 409-25
MeSH Terms: Acetyltransferases, Animals, Apoptosis, Arginase, Arsenicals, Biochemistry, Cells, Cultured, Enzyme Assays, Helicobacter Infections, Helicobacter pylori, Humans, Immunoblotting, Luciferases, Macrophages, Mice, Nitrogen Dioxide, Ornithine Decarboxylase, Oxidoreductases Acting on CH-NH Group Donors, Polyamines, Promoter Regions, Genetic, RNA, Messenger, Transfection
Show Abstract · Added March 5, 2014
Helicobacter pylori is a Gram-negative bacteria that infects the human stomach of half of the world's -population. Colonization is followed by infiltration of the gastric mucosa by lymphocytes and myeloid cells. These cells are activated by various bacterial factors, causing them to produce immune/inflammatory mediators, including reactive nitrogen species and polyamines that contribute to cellular damage and the pathogenesis of H. pylori-associated gastric cancer. In vitro experiments have revealed that H. pylori induces macrophage polyamine production by upregulation of the arginase 2/ornithine decarboxylase (ODC) metabolic pathway and enhances hydrogen peroxide synthesis through the activity of spermidine oxidase (SMO). In this chapter, we present a survey of the methods used to analyze the induction and the role of the enzymes related to polyamine metabolism, i.e., arginase, ODC, and SMO in H. pylori-infected macrophages.
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22 MeSH Terms