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Results: 1 to 4 of 4

Publication Record


A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo.
Yang YP, Ma H, Starchenko A, Huh WJ, Li W, Hickman FE, Zhang Q, Franklin JL, Mortlock DP, Fuhrmann S, Carter BD, Ihrie RA, Coffey RJ
(2017) Cell Rep 19: 1257-1267
MeSH Terms: Adult Stem Cells, Amphiregulin, Animals, Embryo, Mammalian, ErbB Receptors, Genes, Reporter, Green Fluorescent Proteins, Hepatocytes, Intestinal Mucosa, Mice, Microscopy, Fluorescence, Protein Transport, Recombinant Proteins, Transgenes
Show Abstract · Added June 21, 2017
EGF receptor (EGFR) is a critical signaling node throughout life. However, it has not been possible to directly visualize endogenous Egfr in mice. Using CRISPR/Cas9 genome editing, we appended a fluorescent reporter to the C terminus of the Egfr. Homozygous reporter mice appear normal and EGFR signaling is intact in vitro and in vivo. We detect distinct patterns of Egfr expression in progenitor and differentiated compartments in embryonic and adult mice. Systemic delivery of EGF or amphiregulin results in markedly different patterns of Egfr internalization and trafficking in hepatocytes. In the normal intestine, Egfr localizes to the crypt rather than villus compartment, expression is higher in adjacent epithelium than in intestinal tumors, and following colonic injury expression appears in distinct cell populations in the stroma. This reporter, under control of its endogenous regulatory elements, enables in vivo monitoring of the dynamics of Egfr localization and trafficking in normal and disease states.
Copyright © 2017 The Authors. Published by Elsevier Inc. All rights reserved.
1 Communities
5 Members
2 Resources
14 MeSH Terms
Spatial regionalization and heterochrony in the formation of adult pallial neural stem cells.
Dirian L, Galant S, Coolen M, Chen W, Bedu S, Houart C, Bally-Cuif L, Foucher I
(2014) Dev Cell 30: 123-36
MeSH Terms: Adult Stem Cells, Animals, Cell Lineage, Embryonic Stem Cells, Gene Expression Regulation, Developmental, Globus Pallidus, Neural Stem Cells, Neurogenesis, Neuroglia, Neurons, Organ Specificity, Promoter Regions, Genetic, Transcription, Genetic, Zebrafish, Zebrafish Proteins
Show Abstract · Added February 12, 2015
Little is known on the embryonic origin and related heterogeneity of adult neural stem cells (aNSCs). We use conditional genetic tracing, activated in a global or mosaic fashion by cell type-specific promoters or focal laser uncaging, coupled with gene expression analyses and Notch invalidations, to address this issue in the zebrafish adult telencephalon. We report that the germinal zone of the adult pallium originates from two distinct subtypes of embryonic progenitors and integrates two modes of aNSC formation. Dorsomedial aNSCs derive from the amplification of actively neurogenic radial glia of the embryonic telencephalon. On the contrary, the lateral aNSC population is formed by stepwise addition at the pallial edge from a discrete neuroepithelial progenitor pool of the posterior telencephalic roof, activated at postembryonic stages and persisting lifelong. This dual origin of the pallial germinal zone allows the temporally organized building of pallial territories as a patchwork of juxtaposed compartments.
Copyright © 2014 Elsevier Inc. All rights reserved.
0 Communities
1 Members
0 Resources
15 MeSH Terms
Lake-front property: a unique germinal niche by the lateral ventricles of the adult brain.
Ihrie RA, Alvarez-Buylla A
(2011) Neuron 70: 674-86
MeSH Terms: Adult, Adult Stem Cells, Age Factors, Animals, Brain, Cell Differentiation, Germ Layers, Humans, Lateral Ventricles, Stem Cells
Show Abstract · Added August 21, 2012
New neurons and glial cells are generated in an extensive germinal niche adjacent to the walls of the lateral ventricles in the adult brain. The primary progenitors (B1 cells) have astroglial characteristics but retain important neuroepithelial properties. Recent work shows how B1 cells contact all major compartments of this niche. They share the "shoreline" on the ventricles with ependymal cells, forming a unique adult ventricular zone (VZ). In the subventricular zone (SVZ), B1 cells contact transit amplifying (type C) cells, chains of young neurons (A cells), and blood vessels. How signals from these compartments influence the behavior of B1 or C cells remains largely unknown, but recent work highlights growth factors, neurotransmitters, morphogens, and the extracellular matrix as key regulators of this niche. The integration of emerging molecular and anatomical clues forecasts an exciting new understanding of how the germ of youth is actively maintained in the adult brain.
Copyright © 2011 Elsevier Inc. All rights reserved.
1 Communities
1 Members
0 Resources
10 MeSH Terms
beta-Catenin initiates tooth neogenesis in adult rodent incisors.
Liu F, Dangaria S, Andl T, Zhang Y, Wright AC, Damek-Poprawa M, Piccolo S, Nagy A, Taketo MM, Diekwisch TG, Akintoye SO, Millar SE
(2010) J Dent Res 89: 909-14
MeSH Terms: Adult Stem Cells, Animals, Dental Papilla, Enamel Organ, Epithelial Cells, Female, Fibroblast Growth Factor 8, Incisor, Mesenchymal Stem Cells, Mice, Mice, Nude, Odontogenesis, Protein Isoforms, Signal Transduction, Tooth Calcification, Up-Regulation, beta Catenin
Show Abstract · Added January 30, 2013
beta-Catenin signaling is required for embryonic tooth morphogenesis and promotes continuous tooth development when activated in embryos. To determine whether activation of this pathway in the adult oral cavity could promote tooth development, we induced mutation of epithelial beta-catenin to a stabilized form in adult mice. This caused increased proliferation of the incisor tooth cervical loop, outpouching of incisor epithelium, abnormal morphology of the epithelial-mesenchymal junction, and enhanced expression of genes associated with embryonic tooth development. Ectopic dental-like structures were formed from the incisor region following implantation into immunodeficient mice. Thus, forced activation of beta-catenin signaling can initiate an embryonic-like program of tooth development in adult rodent incisor teeth.
0 Communities
1 Members
0 Resources
17 MeSH Terms