SC35-mediated reconstitution of splicing in U2AF-depleted nuclear extract. MacMillan AM, McCaw PS, Crispino JD, Sharp PA (1997) Proc Natl Acad Sci U S A 94: 133-6 Evidence that Myb-related CDC5 proteins are required for pre-mRNA splicing. Burns CG, Ohi R, Krainer AR, Gould KL (1999) Proc Natl Acad Sci U S A 96: 13789-94 Structural characterization of the fission yeast U5.U2/U6 spliceosome complex. Ohi MD, Ren L, Wall JS, Gould KL, Walz T (2007) Proc Natl Acad Sci U S A 104: 3195-200 The Prp19 WD40 domain contains a conserved protein interaction region essential for its function. Vander Kooi CW, Ren L, Xu P, Ohi MD, Gould KL, Chazin WJ (2010) Structure 18: 584-93 Structural and functional analysis of essential pre-mRNA splicing factor Prp19p. Ohi MD, Vander Kooi CW, Rosenberg JA, Ren L, Hirsch JP, Chazin WJ, Walz T, Gould KL (2005) Mol Cell Biol 25: 451-60 A minimal spliceosomal complex A recognizes the branch site and polypyrimidine tract. Query CC, McCaw PS, Sharp PA (1997) Mol Cell Biol 17: 2944-53 Myb-related fission yeast cdc5p is a component of a 40S snRNP-containing complex and is essential for pre-mRNA splicing. McDonald WH, Ohi R, Smelkova N, Frendewey D, Gould KL (1999) Mol Cell Biol 19: 5352-62 Structure of the U6 RNA intramolecular stem-loop harboring an S(P)-phosphorothioate modification. Reiter NJ, Nikstad LJ, Allmann AM, Johnson RJ, Butcher SE (2003) RNA 9: 533-42 Characterization of interactions among the Cef1p-Prp19p-associated splicing complex. Ohi MD, Gould KL (2002) RNA 8: 798-815 The splicing factor PSF is part of a large complex that assembles in the absence of pre-mRNA and contains all five snRNPs. Peng R, Hawkins I, Link AJ, Patton JG (2006) RNA Biol 3: 69-76 Systematic two-hybrid and comparative proteomic analyses reveal novel yeast pre-mRNA splicing factors connected to Prp19. Ren L, McLean JR, Hazbun TR, Fields S, Vander Kooi C, Ohi MD, Gould KL (2011) PLoS One 6: e16719 Structural and functional analyses of the spliceosome requires a multi-disciplinary approach. Ohi MD (2017) Methods 125: 1-2 Structural and functional insights into the N-terminus of Schizosaccharomyces pombe Cdc5. Collier SE, Voehler M, Peng D, Ohi R, Gould KL, Reiter NJ, Ohi MD (2014) Biochemistry 53: 6439-51 Protein phosphatase 2A family members (PP2A and PP6) associate with U1 snRNP and the spliceosome during pre-mRNA splicing. Kamoun M, Filali M, Murray MV, Awasthi S, Wadzinski BE (2013) Biochem Biophys Res Commun 440: 306-11
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