Efficient DNA cassette exchange in mouse embryonic stem cells by staggered positive-negative selection.

Long Q, Shelton KD, Lindner J, Jones JR, Magnuson MA
Genesis. 2004 39 (4): 256-62

PMID: 15286998 · DOI:10.1002/gene.20053

Recombinase-mediated cassette exchange (RMCE), when applied to mouse embryonic stem (ES) cells, promises to increase the ease with which genetic alterations can be introduced into targeted genomic loci in the mouse. However, existing selection strategies for identifying ES cells in which replacement DNA cassettes from a carrier plasmid have been exchanged correctly into a defined locus are suboptimal. Here, we report the generation in mouse ES cells of a loxed cassette acceptor (LCA) allele within the glucokinase (gk) gene locus. Using the gkLCA as a test allele, we developed a staggered positive-negative selection strategy that facilitates efficient identification of ES cell clones in which a DNA replacement cassette from a carrier plasmid has been exchanged correctly into the gkLCA allele. This selection strategy, by facilitating more efficient production of ES cell clones with various replacement DNA cassettes, should accelerate targeted repetitive introduction of gene modifications into the mouse.

2004 Wiley-Liss, Inc.

MeSH Terms (12)

Alleles Animals DNA Primers Embryo, Mammalian Gene Components Gene Targeting Glucokinase Mice Mutagenesis, Insertional Plasmids Recombinases Stem Cells

Connections (10)

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