FUCCI tracking shows cell-cycle-dependent Neurog3 variation in pancreatic progenitors.

Bechard ME, Bankaitis ED, Ustione A, Piston DW, Magnuson MA, Wright CVE
Genesis. 2017 55 (9)

PMID: 28772022 · PMCID: PMC5750046 · DOI:10.1002/dvg.23050

During pancreas organogenesis, Neurog3 endocrine-committing cells are generated from a population of Sox9 mitotic progenitors with only a low level of Neurog3 transcriptional activity (Neurog3 ). Low-level Neurog3 protein, in Neurog3 cells, is required to maintain their mitotic endocrine-lineage-primed status. Herein, we describe a Neurog3-driven FUCCI cell-cycle reporter (Neurog3 ) derived from a Neurog3 BAC transgenic reporter that functions as a loxed cassette acceptor (LCA). In cycling Sox9 Neurog3 progenitors, the majority of cells in S-G -M phases have undetectable levels of Neurog3 with increased expression of endocrine progenitor markers, while those in G have low Neurog3 levels with increased expression of endocrine differentiation markers. These findings support a model in which variations in Neurog3 protein levels are coordinated with cell-cycle phase progression in Neurog3 progenitors with entrance into G triggering a concerted effort, beyond increasing Neurog3 levels, to maintain an endocrine-lineage-primed state by initiating expression of the downstream endocrine differentiation program prior to endocrine-commitment.

© 2017 Wiley Periodicals, Inc.

MeSH Terms (9)

Animals Basic Helix-Loop-Helix Transcription Factors Cell Cycle Cells, Cultured Embryonic Stem Cells Green Fluorescent Proteins Islets of Langerhans Mice Nerve Tissue Proteins

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