Expression of the rat brain creatine transporter in situ and in transfected HeLa cells.

Saltarelli MD, Bauman AL, Moore KR, Bradley CC, Blakely RD
Dev Neurosci. 1996 18 (5-6): 524-34

PMID: 8940628 · DOI:10.1159/000111450

Using degenerate oligonucleotide probes encoding conserved regions of the gamma-aminobutyric acid/norepinephrine transporter gene family, we have cloned a rat brain cDNA encoding a creatine transporter (rCREAT). rCREAT encodes a highly hydrophobic, 635-amino-acid protein possessing 12 potential transmembrane domains and canonical sites for N-linked glycosylation and protein phosphorylation. Transfection of rCREAT cDNA into mammalian cells results in the expression of [14C]creatine uptake, which is blocked by low micromolar concentrations of recognized creatine uptake inhibitors. Two rCREAT mRNAs are expressed in the rat brain, retina, kidney and heart. Whole-brain rCREAT mRNAs demonstrate a marked postnatal rise to steady-state adult levels. In situ hybridization studies indicate a widespread, differential rCREAT mRNA expression in adult rat brain, with high expression noted over myelinated fiber tracts, cerebellar granule cells, hippocampal pyramidal cells, brainstem nuclei and endothelial cells of the choroid plexus. These studies will allow the development of new molecular probes useful for defining the creatine transporter's cellular expression pattern, function in ATP homeostasis and association with disorders of cellular energy metabolism.

MeSH Terms (16)

Amino Acid Sequence Animals Animals, Newborn Base Sequence Brain Carrier Proteins Gene Expression HeLa Cells Humans Membrane Transport Proteins Molecular Sequence Data Rats RNA Substrate Specificity Tissue Distribution Transfection

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