Peptide-conjugated quantum dots: imaging the angiotensin type 1 receptor in living cells.

Tomlinson ID, Mason JN, Blakely RD, Rosenthal SJ
Methods Mol Biol. 2005 303: 51-60

PMID: 15923674 · DOI:10.1385/1-59259-901-X:051

Peptide-quantum dot conjugates have been prepared by attaching angiotensin II (Ang II) to cadmium selenide/zinc sulfide core-shell nanocrystals using an 1-[3-(Dimethyamino)propyl]-3-ethylcarbo diimide hydrochloride (EDC) coupling. These conjugates have been used to image angiotensin I-expressing Chinese hamster ovary (CHO) cells in vitro. When CHO cells were incubated with Ang II before incubating with Ang II-conjugated quantum dots, we were able to block the binding of the dots. The Ang II-quantum dot conjugates did not bind to parental cells and showed similar staining patterns when compared with a commercially available Ang II Alexa 488 conjugate.

MeSH Terms (9)

Angiotensin II Animals CHO Cells Cricetinae Diagnostic Imaging Nanotechnology Peptides Quantum Dots Receptors, Angiotensin

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