Acetylcholine is an autocrine or paracrine hormone synthesized and secreted by airway bronchial epithelial cells.

Proskocil BJ, Sekhon HS, Jia Y, Savchenko V, Blakely RD, Lindstrom J, Spindel ER
Endocrinology. 2004 145 (5): 2498-506

PMID: 14764638 · DOI:10.1210/en.2003-1728

The role of acetylcholine (ACh) as a key neurotransmitter in the central and peripheral nervous system is well established. However, the role of ACh may be broader because ACh may also function as an autocrine or paracrine signaling molecule in a variety of nonneuronal tissues. To begin to establish ACh of nonneuronal origin as a paracrine hormone in lung, we have examined neonatal and adult monkey bronchial epithelium for the components involved in nicotinic cholinergic signaling. Using immunohistochemistry and RT-PCR, we have demonstrated in lung bronchial epithelial cells (BECs) expression of choline acetyltransferase, the vesicular ACh transporter, the choline high-affinity transporter, alpha7, alpha4, and beta2 nicotinic ACh receptor (nAChR) subunits, and the nAChR accessory protein lynx1. Confocal microscopy demonstrates that these factors are expressed in epithelial cells and are clearly distinct from neighboring nerve fibers. Confirmation of RNA identity has been confirmed by partial sequence analysis of PCR products and by cDNA cloning. Primary culture of BECs confirms the synthesis and secretion of ACh and the activity of cholinesterases. Thus, ACh meets all the criteria for an autocrine/paracrine hormone in lung bronchial epithelium. The nonneuronal cholinergic signaling pathway in lung provides a potentially important target for cholinergic drugs. This pathway may also explain some of the effects of nicotine on fetal development and also provides additional mechanisms by which smoking affects lung cancer growth and development.

MeSH Terms (17)

Acetylcholine Aging Animals Animals, Newborn Bronchi Cells, Cultured Choline O-Acetyltransferase Chromatography, High Pressure Liquid Epithelial Cells Immunohistochemistry Macaca mulatta Membrane Transport Proteins Microscopy, Confocal Neuropeptides Receptors, Nicotinic Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger

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