Philip Kingsley
Last active: 3/12/2020

Assay of Endocannabinoid Oxidation by Cyclooxygenase-2.

Kudalkar SN, Kingsley PJ, Marnett LJ
Methods Mol Biol. 2016 1412: 205-15

PMID: 27245906 · PMCID: PMC5289390 · DOI:10.1007/978-1-4939-3539-0_21

The endocannabinoids, 2-arachidonoylglycerol (2-AG) and arachidonylethanolamide (AEA), are endogenous ligands for the cannabinoid receptors (CB1 and CB2) and are implicated in a wide array of physiological processes. These neutral arachidonic acid (AA) derivatives have been identified as efficient substrates for the second isoform of the cyclooxygenase enzyme (COX-2). A diverse family of prostaglandin glycerol esters (PG-Gs) and prostaglandin ethanolamides (PG-EAs) is generated by the action of COX-2 (and downstream prostaglandin synthases) on 2-AG and AEA. As the biological importance of the endocannabinoid system becomes more apparent, there is a tremendous need for robust, sensitive, and efficient analytical methodology for the endocannabinoids and their metabolites. In this chapter, we describe methodology suitable for carrying out oxygenation of endocannabinoids by COX-2, and analysis of products of endocannabinoid oxygenation by COX-2 and of endocannabinoids themselves from in vitro and cell assays.

MeSH Terms (16)

Animals Arachidonic Acid Arachidonic Acids Biological Assay Cell Line Chromatography, Liquid Cyclooxygenase 2 Endocannabinoids Glycerides In Vitro Techniques Macrophages Mice Oxidation-Reduction Polyunsaturated Alkamides Substrate Specificity Tandem Mass Spectrometry

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