Philip Kingsley
Last active: 3/12/2020

Publication

N-acylphosphatidylethanolamine-hydrolyzing phospholipase D is an important determinant of uterine anandamide levels during implantation.

Guo Y, Wang H, Okamoto Y, Ueda N, Kingsley PJ, Marnett LJ, Schmid HH, Das SK, Dey SK
J Biol Chem. 2005 280 (25): 23429-32

PMID: 15890658 · DOI:10.1074/jbc.C500168200

Implantation requires reciprocal interaction between blastocysts and a receptive uterus. In mice, one important player in this dialogue involves endocannabinoid signaling via cannabinoid receptor CB1. Anandamide is an endogenous cannabinoid ligand, and its levels are spatiotemporally regulated in the uterus during early pregnancy, showing lower levels in the receptive uterus and at the implantation site. However, the mechanism by which differential uterine anandamide gradients are established under different pregnancy status is not clearly understood. Using multiple approaches, we show here that uterine anandamide levels conducive to implantation are primarily regulated by spatiotemporal expression of Nape-Pld, the gene encoding N-acylphosphatidylethanolamine-hydrolyzing phospholipase D that generates anandamide. The expression is well correlated with its activity and anandamide levels. This study is clinically relevant, since elevated anandamide levels in peripheral circulation are associated with spontaneous pregnancy failure in women.

MeSH Terms (19)

Animals Arachidonic Acids Base Sequence DNA Primers Down-Regulation Embryo Implantation Endocannabinoids Estrogens Female Hydrolysis In Situ Hybridization Male Mice Phosphoric Diester Hydrolases Polyunsaturated Alkamides Pregnancy Progesterone Reverse Transcriptase Polymerase Chain Reaction Uterus

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